Abstract
In transgenic tobacco, pea Ferredoxin-1 (Fed-1) mRNA accumulates rapidly in response to photosynthesis even when the transgene is driven by a constitutive promoter. To investigate the role of photosynthesis on Fed-1 mRNA stability, we used the tetracycline repressible Top10 promoter system to specifically shut off transcription of the Fed-1 transgene. The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light and a half-life of only 1.2 hr in the dark or in the presence of the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). These data indicate that cessation of photosynthesis, either by darkness or DCMU results in a destabilization of the Fed-1 mRNA. Furthermore, the Fed-1 mRNA half-life is reduced immediately upon transfer to darkness, suggesting that Fed-1 mRNA destabilization is a primary response to photosynthesis rather than a secondary response to long-term dark adaptation. Finally, the two different methods for efficient tetracycline delivery reported here generally should be useful for half-life measurements of other mRNAs in whole plants.
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Claims 7
Claim · model-written summary
Pea Fed-1 mRNA rapidly accumulates with photosynthesis in transgenic tobacco despite a constitutive promoter.
- Taxon
- transgenic tobacco
Nicotiana tabacume0 - Gene
- pea Ferredoxin-1 (Fed-1)e0
- Outcome
- mRNA accumulatese0
- Direction
- accumulates rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco, pea Ferredoxin-1 (Fed-1) mRNA accumulates rapidly in response to photosynthesis even when the transgene is driven by a constitutive promoter”
Claim · model-written summary
Fed-1 mRNA half-life was approximately 2.4 hr in light.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Gene
- Fed-1e0
- Outcome
- half-lifee0
- Value
- approximately 2.4 hre0
- Setting
- in the lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light”
e1“In transgenic tobacco, pea Ferredoxin-1 (Fed-1) mRNA accumulates rapidly in response to photosynthesis even when the transgene is driven by a constitutive promoter”
Claim · model-written summary
Fed-1 mRNA half-life was 1.2 hr in darkness.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Treatment
- in the darke0
- Gene
- Fed-1e0
- Outcome
- half-lifee0
- Direction
- onlye0
- Value
- 1.2 hre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light and a half-life of only 1.2 hr in the dark”
e1“In transgenic tobacco”
Claim · model-written summary
Fed-1 mRNA half-life was 1.2 hr with DCMU inhibition of photosynthetic electron transport.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Treatment
- photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)e0
- Gene
- Fed-1e0
- Outcome
- half-lifee0
- Direction
- onlye0
- Value
- 1.2 hre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light and a half-life of only 1.2 hr in the dark or in the presence of the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)”
e1“In transgenic tobacco”
Claim · model-written summary
Fed-1 mRNA half-life dropped immediately after transfer to darkness.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Treatment
- transfer to darknesse0
- Gene
- Fed-1e0
- Outcome
- Fed-1 mRNA half-lifee0
- Direction
- reducede0
- Qualifier
- immediatelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Fed-1 mRNA half-life is reduced immediately upon transfer to darkness”
e1“In transgenic tobacco”
Claim · model-written summary
The immediate decrease suggests Fed-1 mRNA destabilization is a primary response to photosynthesis.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Gene
- Fed-1e0
- Outcome
- Fed-1 mRNA destabilizatione0
- Qualifier
- primary response to photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fed-1 mRNA destabilization is a primary response to photosynthesis rather than a secondary response to long-term dark adaptation”
e1“In transgenic tobacco”
Claim · model-written summary
The reported tetracycline-delivery methods should help measure other mRNA half-lives in whole plants.
- Taxon
- transgenic tobaccoNicotiana tabacume1
- Outcome
- half-life measurements of other mRNAs in whole plantse0
- Value
- two different methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two different methods for efficient tetracycline delivery reported here generally should be useful for half-life measurements of other mRNAs in whole plants”
e1“In transgenic tobacco”
The same questions in other papers
light · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.