Abstract
Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to the mother plant during seed development (endogenous nitrate). Transcript profiling of imbibed seeds treated with or without nitrate revealed that exogenous nitrate led to a higher expression of nitrate-responsive genes, whereas endogenous nitrate led to a profile similar to that of stratified or after-ripened seeds. Profiling experiments indicated that the expression of the ABA catabolic gene CYP707A2 was regulated by exogenous nitrate. The cyp707a2-1 mutant failed to reduce seed ABA levels in response to both endogenous and exogenous nitrate. In contrast, both endogenous and exogenous nitrate reduced ABA levels of the wild-type and cyp707a1-1 mutant seeds. The CYP707A2 mRNA levels in developing siliques were positively correlated with different nitrate doses applied to the mother plants. This was consistent with a role of the CYP707A2 gene in controlling seed ABA levels in response to endogenous nitrate. The cyp707a2-1 mutant was less sensitive to exogenous nitrate for breaking seed dormancy. Altogether, our data underline the central role of the CYP707A2 gene in the nitrate-mediated control of ABA levels during seed development and germination.
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Claims 13
Claim · model-written summary
Exogenous nitrate lowered ABA in imbibed seeds.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- imbibed seedse0
- Treatment
- exogenous nitratee0
- Outcome
- levels of ABAe0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate).”
t0“Arabidopsis”
Claim · model-written summary
Endogenous nitrate lowered ABA in dry seeds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- dry seedse0
- Treatment
- endogenous nitratee0
- Outcome
- ABA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate also reduced ABA levels in dry seeds when provided to the mother plant during seed development (endogenous nitrate).”
t0“Arabidopsis”
Claim · model-written summary
Exogenous nitrate increased expression of nitrate-responsive genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- exogenous nitratee0
- Gene
- nitrate-responsive genese0
- Outcome
- expression of nitrate-responsive genese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous nitrate led to a higher expression of nitrate-responsive genes”
t0“Arabidopsis”
Claim · model-written summary
Endogenous nitrate gave a transcript profile similar to stratified or after-ripened seeds.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- endogenous nitratee0
- Outcome
- profilee0
- Comparator
- stratified or after-ripened seedse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous nitrate led to a profile similar to that of stratified or after-ripened seeds.”
t0“Arabidopsis”
Claim · model-written summary
Exogenous nitrate regulated CYP707A2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- exogenous nitratee0
- Gene
- CYP707A2e0
- Outcome
- expression of the ABA catabolic gene CYP707A2e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the ABA catabolic gene CYP707A2 was regulated by exogenous nitrate.”
t0“Arabidopsis”
Claim · model-written summary
The cyp707a2-1 mutant failed to reduce seed ABA under endogenous nitrate.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp707a2-1 mutante0
- Treatment
- endogenouse0
- Outcome
- seed ABA levelse0
- Direction
- failed to reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cyp707a2-1 mutant failed to reduce seed ABA levels in response to both endogenous and exogenous nitrate.”
t0“Arabidopsis”
Claim · model-written summary
The cyp707a2-1 mutant failed to reduce seed ABA under exogenous nitrate.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp707a2-1 mutante0
- Treatment
- exogenous nitratee0
- Outcome
- seed ABA levelse0
- Direction
- failed to reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cyp707a2-1 mutant failed to reduce seed ABA levels in response to both endogenous and exogenous nitrate.”
t0“Arabidopsis”
Claim · model-written summary
Endogenous nitrate reduced ABA in wild-type seeds.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-typee0
- Treatment
- endogenouse0
- Outcome
- ABA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both endogenous and exogenous nitrate reduced ABA levels of the wild-type and cyp707a1-1 mutant seeds.”
t0“Arabidopsis”
Claim · model-written summary
Exogenous nitrate reduced ABA in wild-type seeds.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-typee0
- Treatment
- exogenous nitratee0
- Outcome
- ABA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both endogenous and exogenous nitrate reduced ABA levels of the wild-type and cyp707a1-1 mutant seeds.”
t0“Arabidopsis”
Claim · model-written summary
Endogenous nitrate reduced ABA in cyp707a1-1 seeds.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp707a1-1 mutante0
- Treatment
- endogenouse0
- Outcome
- ABA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both endogenous and exogenous nitrate reduced ABA levels of the wild-type and cyp707a1-1 mutant seeds.”
t0“Arabidopsis”
Claim · model-written summary
Exogenous nitrate reduced ABA in cyp707a1-1 seeds.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp707a1-1 mutante0
- Treatment
- exogenous nitratee0
- Outcome
- ABA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both endogenous and exogenous nitrate reduced ABA levels of the wild-type and cyp707a1-1 mutant seeds.”
t0“Arabidopsis”
Claim · model-written summary
CYP707A2 mRNA in developing siliques correlated positively with nitrate dose to mother plants.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- developing siliquese0
- Treatment
- different nitrate dosese0
- Gene
- CYP707A2e0
- Outcome
- CYP707A2 mRNA levelse0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CYP707A2 mRNA levels in developing siliques were positively correlated with different nitrate doses applied to the mother plants.”
t0“Arabidopsis”
Claim · model-written summary
The cyp707a2-1 mutant was less responsive to exogenous nitrate for dormancy release.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp707a2-1 mutante0
- Treatment
- exogenous nitratee0
- Outcome
- breaking seed dormancye0
- Direction
- less sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cyp707a2-1 mutant was less sensitive to exogenous nitrate for breaking seed dormancy.”
t0“Arabidopsis”
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12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.