Abstract
The objective of the current work was to establish the degree to which the effects of carbon and nitrogen availability on Arabidopsis seedling growth and development are due to these nutrients acting independently or together. Growth of seedlings on low (0.1 mM) nitrogen results in a significant reduction of seedling and cotyledon size, fresh weight, chlorophyll, and anthocyanin content but a slight increase in endogenous sugars. The addition of 100 mM sucrose (Suc) to the nitrogen-depleted growth media results in a further reduction in cotyledon size and chlorophyll content and an overall increase in anthocyanins and endogenous sugars. Storage lipid breakdown is almost completely blocked in seedlings grown on low nitrogen and 100 mM Suc and is significantly inhibited when seedlings are grown on either low nitrogen or high Suc. Carbohydrate repression of photosynthetic gene expression can only be observed under low nitrogen conditions. Low (0.1 mM) nitrogen in the absence of exogenous carbohydrate results in a significant decrease in chlorophyll a/b-binding protein and ribulose bisphosphate carboxylase small subunit gene transcript levels. Thus, carbon to nitrogen ratio rather than carbohydrate status alone appears to play the predominant role in regulating various aspects of seedling growth including storage reserve mobilization and photosynthetic gene expression.
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Claims 10
Claim · model-written summary
Low nitrogen significantly reduced seedling and cotyledon size and fresh weight.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- low (0.1 mM) nitrogene0
- Outcome
- seedling and cotyledon size, fresh weighte0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth of seedlings on low (0.1 mM) nitrogen results in a significant reduction of seedling and cotyledon size, fresh weight”
t0“Arabidopsis”
Claim · model-written summary
Low nitrogen significantly reduced chlorophyll and anthocyanin content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low (0.1 mM) nitrogene0
- Outcome
- chlorophyll, and anthocyanin contente0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low (0.1 mM) nitrogen results in a significant reduction of seedling and cotyledon size, fresh weight, chlorophyll, and anthocyanin content”
t0“Arabidopsis”
Claim · model-written summary
Low nitrogen slightly increased endogenous sugars.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low (0.1 mM) nitrogene1
- Outcome
- endogenous sugarse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a slight increase in endogenous sugars”
e1“low (0.1 mM) nitrogen”
t0“Arabidopsis”
Claim · model-written summary
Adding sucrose under nitrogen depletion further reduced cotyledon size and chlorophyll content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 100 mM sucrose (Suc)e0
- Outcome
- cotyledon size and chlorophyll contente0
- Direction
- further reductione0
- Comparator
- nitrogen-depleted growth mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 100 mM sucrose (Suc) to the nitrogen-depleted growth media results in a further reduction in cotyledon size and chlorophyll content”
t0“Arabidopsis”
Claim · model-written summary
Adding sucrose under nitrogen depletion increased anthocyanins and endogenous sugars.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 100 mM sucrose (Suc)e1
- Outcome
- anthocyanins and endogenous sugarse0
- Direction
- increasee0
- Comparator
- nitrogen-depleted growth mediae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an overall increase in anthocyanins and endogenous sugars”
e1“The addition of 100 mM sucrose (Suc) to the nitrogen-depleted growth media”
t0“Arabidopsis”
Claim · model-written summary
Combined low nitrogen and sucrose almost completely blocked storage lipid breakdown.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low nitrogen and 100 mM Suce0
- Outcome
- Storage lipid breakdowne0
- Direction
- almost completely blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Storage lipid breakdown is almost completely blocked in seedlings grown on low nitrogen and 100 mM Suc”
t0“Arabidopsis”
Claim · model-written summary
Low nitrogen alone significantly inhibited storage lipid breakdown.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low nitrogene0
- Outcome
- Storage lipid breakdowne1
- Direction
- significantly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is significantly inhibited when seedlings are grown on either low nitrogen or high Suc”
e1“Storage lipid breakdown”
t0“Arabidopsis”
Claim · model-written summary
High sucrose alone significantly inhibited storage lipid breakdown.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- high Suce0
- Outcome
- Storage lipid breakdowne1
- Direction
- significantly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is significantly inhibited when seedlings are grown on either low nitrogen or high Suc”
e1“Storage lipid breakdown”
t0“Arabidopsis”
Claim · model-written summary
Carbohydrate repression of photosynthetic gene expression occurred only under low nitrogen.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low nitrogen conditionse0
- Outcome
- photosynthetic gene expressione0
- Direction
- repressione0
- Qualifier
- onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carbohydrate repression of photosynthetic gene expression can only be observed under low nitrogen conditions”
t0“Arabidopsis”
Claim · model-written summary
Low nitrogen without exogenous carbohydrate significantly reduced two photosynthetic gene transcripts.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Low (0.1 mM) nitrogene0
- Gene
- chlorophyll a/b-binding protein and ribulose bisphosphate carboxylase small subunite0
- Outcome
- gene transcript levelse0
- Direction
- decreasee0
- Qualifier
- in the absence of exogenous carbohydratee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low (0.1 mM) nitrogen in the absence of exogenous carbohydrate results in a significant decrease in chlorophyll a/b-binding protein and ribulose bisphosphate carboxylase small subunit gene transcript levels”
t0“Arabidopsis”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.