Abstract
ABSTRACT Transgenic tobacco plants (Tetr) expressing the mutant etr1-1 gene from Arabidopsis thaliana are insensitive to ethylene and develop symptoms of wilting and stem rot when grown in nonautoclaved soil. Several isolates of Fusarium, Thielaviopsis, and Pythium were recovered from stems of diseased Tetr plants. Inoculation with each of these isolates of 6-week-old plants growing in autoclaved soil caused disease in Tetr plants but not in nontransformed plants. Also, when 2-week-old seedlings were used, nontransformed tobacco appeared nonsusceptible to the Fusarium isolates, whereas Tetr seedlings did develop disease. Tetr seedlings were not susceptible to several nonhost Fusarium isolates. In contrast to results with Fusarium isolates, inoculation of 2-week-old seedlings with a Thielaviopsis isolate resulted in equal symptom development of nontransformed and Tetr tobacco. In order to explore the potential range of pathogens to which Tetr tobacco plants display enhanced susceptibility, the pathogenicity of several root and leaf pathogens was tested. Tetr plants were more susceptible to the necrotrophic fungi Botrytis cinerea and Cercospora nicotianae and the bacterium Erwinia carotovora, but only marginally more to the bacterium Ralstonia solanacearum. In contrast, the biotrophic fungus Oidium neolycopersici, the oomycete Peronospora tabacina, and Tobacco mosaic virus caused similar or less severe symptoms on Tetr plants than on nontransformed plants. Total peroxidase activity of Tetr plants was lower than that of nontransformed plants, suggesting a role for peroxidases in resistance against necrotrophic microorganisms. A comparable range of pathogens was examined on Arabidopsis and its ethylene-insensitive mutants etr1-1 and ein2-1. With the exception of one Fusarium isolate, ethylene insensitivity increased susceptibility of Arabidopsis plants to a similar spectrum of necrotizing pathogens as in tobacco. Thus, both ethylene-insensitive tobacco and Arabidopsis plants appear to be impaired in their resistance to necrotrophic pathogens.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Inoculated 6-week-old Tetr tobacco plants in autoclaved soil developed disease, while nontransformed plants did not.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- Tetr plantse1
- Organ / stage
- 6-week-olde1
- Treatment
- Inoculation with each of these isolatese1
- Second interactor
- Thielaviopsis
Thielaviopsise0pathogen - Outcome
- diseasee1
- Qualifier
- Fusarium, Thielaviopsis, and Pythiume0
- Comparator
- nontransformed plantse1
- Setting
- autoclaved soile1
FusariumNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e1“Inoculation with each of these isolates of 6-week-old plants growing in autoclaved soil caused disease in Tetr plants but not in nontransformed plants”
e0“Several isolates of Fusarium, Thielaviopsis, and Pythium were recovered from stems of diseased Tetr plants”
Claim · model-written summary
Tetr tobacco seedlings developed disease from Fusarium isolates, while nontransformed tobacco seedlings were nonsusceptible.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr seedlingse2
- Organ / stage
- seedlingse2
- Treatment
- Fusarium isolatese2
- Outcome
- diseasee2
- Comparator
- nontransformed tobaccoe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e2“nontransformed tobacco appeared nonsusceptible to the Fusarium isolates, whereas Tetr seedlings did develop disease”
Claim · model-written summary
Thielaviopsis isolate caused equal symptom development on nontransformed and Tetr tobacco seedlings.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- 2-week-old seedlingse3
- Treatment
- Thielaviopsis isolatee3
- Interactor
- ThielaviopsisThielaviopsise3pathogen
- Outcome
- symptom developmente3
- Direction
- equale3
- Comparator
- nontransformede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e3“inoculation of 2-week-old seedlings with a Thielaviopsis isolate resulted in equal symptom development of nontransformed and Tetr tobacco”
Claim · model-written summary
Tetr tobacco seedlings were not susceptible to several nonhost Fusarium isolates.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr seedlingse4
- Treatment
- nonhost Fusarium isolatese4
- Outcome
- susceptiblee4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e4“Tetr seedlings were not susceptible to several nonhost Fusarium isolates”
Claim · model-written summary
Tetr tobacco plants were more susceptible to Botrytis cinerea.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse5
- Interactor
- Botrytis cinerea
Botrytis cinereae5pathogen - Outcome
- susceptiblee5
- Direction
- moree5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e5“Tetr plants were more susceptible to the necrotrophic fungi Botrytis cinerea and Cercospora nicotianae and the bacterium Erwinia carotovora”
Claim · model-written summary
Tetr tobacco plants were more susceptible to Cercospora nicotianae.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse5
- Interactor
- Cercospora nicotianaeCercospora nicotianaee5pathogen
- Outcome
- susceptiblee5
- Direction
- moree5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e5“Tetr plants were more susceptible to the necrotrophic fungi Botrytis cinerea and Cercospora nicotianae and the bacterium Erwinia carotovora”
Claim · model-written summary
Tetr tobacco plants were more susceptible to Erwinia carotovora.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse5
- Interactor
- Erwinia carotovora
Pectobacterium carotovorume5pathogen - Outcome
- susceptiblee5
- Direction
- moree5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e5“Tetr plants were more susceptible to the necrotrophic fungi Botrytis cinerea and Cercospora nicotianae and the bacterium Erwinia carotovora”
Claim · model-written summary
Tetr tobacco plants showed similar or less severe symptoms from Oidium neolycopersici than nontransformed plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse7
- Interactor
- Oidium neolycopersiciErysiphe neolycopersicie7pathogen
- Outcome
- symptomse7
- Direction
- similar or less severee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e7“the biotrophic fungus Oidium neolycopersici, the oomycete Peronospora tabacina, and Tobacco mosaic virus caused similar or less severe symptoms on Tetr plants than on nontransformed plants”
Claim · model-written summary
Tetr tobacco plants showed similar or less severe symptoms from Peronospora tabacina than nontransformed plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse7
- Interactor
- Peronospora tabacina
Peronospora tabacinae7pathogen - Outcome
- symptomse7
- Direction
- similar or less severee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e7“the biotrophic fungus Oidium neolycopersici, the oomycete Peronospora tabacina, and Tobacco mosaic virus caused similar or less severe symptoms on Tetr plants than on nontransformed plants”
Claim · model-written summary
Tetr tobacco plants showed similar or less severe symptoms from Tobacco mosaic virus than nontransformed plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse7
- Interactor
- Tobacco mosaic virus
Tobacco mosaic viruse7pathogen - Outcome
- symptomse7
- Direction
- similar or less severee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e7“the biotrophic fungus Oidium neolycopersici, the oomycete Peronospora tabacina, and Tobacco mosaic virus caused similar or less severe symptoms on Tetr plants than on nontransformed plants”
Claim · model-written summary
Total peroxidase activity of Tetr tobacco plants was lower than that of nontransformed plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Tetr plantse0
- Outcome
- Total peroxidase activitye0
- Direction
- lowere0
- Comparator
- nontransformed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e0“Total peroxidase activity of Tetr plants was lower than that of nontransformed plants”
Claim · model-written summary
Peroxidases may play a role in resistance of tobacco against necrotrophic microorganisms.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- peroxidasese0
- Outcome
- resistance against necrotrophic microorganismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ethylene-Insensitive Tobacco Shows Differentially Altered Susceptibility”
e0“suggesting a role for peroxidases in resistance against necrotrophic microorganisms”
Claim · model-written summary
Ethylene insensitivity increased susceptibility of Arabidopsis to a similar spectrum of necrotizing pathogens.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- ethylene insensitivitye0
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene insensitivity increased susceptibility of Arabidopsis plants to a similar spectrum of necrotizing pathogens”
The same questions in other papers
ethene · disease · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- positive
- positive
7 papers more in this group
See all 11 papers →Fusarium · disease · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.