Abstract
To clarify how the transcriptionally inactive chromatin of dormant wheat seed embryos becomes active during germination, we studied two kinds of chromatin-associated proteins: histones and nonhistone proteins found in wheat germ.Two major nonhistone proteins were solubilized from purified germ chromatin with 5 molar urea, and were separated from histones and chromosomal RNA by BioRex-70 resin and diethylaminoethyl-cellulose chromatography, respectively. We found that purified 5 molar urea-soluble nonhistone proteins, including the two major nonhistone proteins, had no effect on the transcription of native wheat DNA.Two kinds of chromatins were reconstituted by gradient dialysis from a mixture of DNA, germ histones, and nonhistone proteins derived from germs or germinated seedlings. Reconstituted chromatins had a 1.4- to 1.6-fold higher protein content than DNA content, protein components similar to native chromatin, and had only about 17 to 25% and 37% the transcriptional activities of native seedling and germ chromatins, respectively. Using the transcriptional activity of chromatin reconstituted from DNA and histones alone as a standard, one kind of reconstituted chromatin containing nonhistone proteins of germ was only about one-fourth as active as the standard. Another with nonhistone proteins from seedlings was about 1.3 to 1.5 times more active than the standard.The ratio of histones to DNA content is approximately 1.3 during germination, but the proportion of histone H1 to the total histones is reduced.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Two major nonhistone proteins were isolated from wheat germ chromatin.
- Taxon
- Wheat
Triticum aestivumt0 - Organ / stage
- germe0
- Outcome
- Two major nonhistone proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two major nonhistone proteins were solubilized from purified germ chromatin with 5 molar urea”
t0“Wheat Germination”
Claim · model-written summary
Purified urea-soluble nonhistone proteins did not affect transcription of native wheat DNA.
- Taxon
- wheatTriticum aestivume0
- Treatment
- purified 5 molar urea-soluble nonhistone proteinse0
- Outcome
- transcription of native wheat DNAe0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“purified 5 molar urea-soluble nonhistone proteins, including the two major nonhistone proteins, had no effect on the transcription of native wheat DNA.”
Claim · model-written summary
Reconstituted chromatins had 1.4- to 1.6-fold more protein than DNA.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- protein contente0
- Direction
- highere0
- Value
- 1.4- to 1.6-folde0
- Comparator
- DNA contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“Reconstituted chromatins had a 1.4- to 1.6-fold higher protein content than DNA content”
Claim · model-written summary
Reconstituted chromatin had protein components similar to native chromatin.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- protein components similar to native chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“protein components similar to native chromatin”
Claim · model-written summary
Reconstituted chromatins had 17 to 25% of native seedling chromatin transcriptional activity.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- transcriptional activitiese0
- Direction
- onlye0
- Value
- 17 to 25%e0
- Comparator
- native seedlinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“had only about 17 to 25% and 37% the transcriptional activities of native seedling and germ chromatins, respectively”
Claim · model-written summary
Reconstituted chromatins had 37% of native germ chromatin transcriptional activity.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- transcriptional activitiese0
- Direction
- onlye0
- Value
- 37%e0
- Comparator
- germ chromatinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“had only about 17 to 25% and 37% the transcriptional activities of native seedling and germ chromatins, respectively”
Claim · model-written summary
Chromatin with germ nonhistone proteins was one-fourth as active as chromatin with DNA and histones alone.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- nonhistone proteins of germe0
- Outcome
- transcriptional activitye0
- Direction
- onlye0
- Value
- one-fourthe0
- Comparator
- chromatin reconstituted from DNA and histones alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“Using the transcriptional activity of chromatin reconstituted from DNA and histones alone as a standard, one kind of reconstituted chromatin containing nonhistone proteins of germ was only about one-fourth as active as the standard.”
Claim · model-written summary
Chromatin with seedling nonhistone proteins was 1.3 to 1.5 times as active as the DNA and histone standard.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- nonhistone proteins from seedlingse0
- Outcome
- more activee0
- Direction
- more activee0
- Value
- 1.3 to 1.5 timese0
- Comparator
- chromatin reconstituted from DNA and histones alonee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“Another with nonhistone proteins from seedlings was about 1.3 to 1.5 times more active than the standard.”
e1“chromatin reconstituted from DNA and histones alone as a standard”
Claim · model-written summary
The histone to DNA ratio was approximately 1.3 during germination.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- germinatione0
- Outcome
- ratio of histones to DNA contente0
- Value
- approximately 1.3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“The ratio of histones to DNA content is approximately 1.3 during germination”
Claim · model-written summary
The proportion of histone H1 among total histones declined during germination.
- Taxon
- WheatTriticum aestivumt0
- Gene
- histone H1e0
- Outcome
- proportion of histone H1 to the total histonese0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germination”
e0“the proportion of histone H1 to the total histones is reduced.”
The same questions in other papers
nitrogen · molecular: expression · Triticum aestivum (wheat)Positive and negative results
10 papers in the same direction as this paper · 26 in the opposite direction
- positive
- positive
- positive
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.