Abstract
Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.
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Claims 10
Claim · model-written summary
Silencing StPUB17 in potato by RNAi enhanced P. infestans leaf colonization
- Taxon
- potato
Solanum tuberosume1 - Treatment
- Silencing StPUB17 in potato by RNAie1
- Interactor
- P. infestans
Phytophthora infestanse1pathogen - Gene
- StPUB17e1
- Outcome
- leaf colonizatione1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e1“Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization”
Claim · model-written summary
Silencing NbPUB17 in Nicotiana benthamiana by VIGS enhanced P. infestans leaf colonization
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS)e1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- NbPUB17e1
- Outcome
- leaf colonizatione1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization”
Claim · model-written summary
Silencing PUB17 attenuated PTI transcriptional responses activated by flg22
- Taxon
- potatoSolanum tuberosume0
- Treatment
- silencing PUB17e2
- Outcome
- PAMP-triggered immunity (PTI) transcriptional responses activated by flg22e2
- Direction
- attenuatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e2“PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17”
Claim · model-written summary
Silencing PUB17 attenuated CF4/Avr4-mediated PCD
- Taxon
- potatoSolanum tuberosume0
- Treatment
- silencing PUB17e2
- Gene
- CF4/Avr4e2
- Outcome
- CF4/Avr4-mediated PCDe2
- Direction
- attenuatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e2“PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17”
Claim · model-written summary
Silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1
- Taxon
- potatoSolanum tuberosume0
- Treatment
- silencing PUB17e3
- Interactor
- P. infestansPhytophthora infestanse3pathogen
- Outcome
- PCD triggered by P. infestans PAMP INF1e3
- Direction
- did not compromisee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e3“However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17”
Claim · model-written summary
Silencing PUB17 did not compromise PCD triggered by co-expression of R3a/AVR3a
- Taxon
- potatoSolanum tuberosume0
- Treatment
- silencing PUB17e3
- Gene
- R3a/AVR3ae3
- Outcome
- PCDe3
- Direction
- did not compromisee3
- Qualifier
- co-expression of R3a/AVR3ae3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e3“However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17”
Claim · model-written summary
Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant suppressed CF4-mediated cell death
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutante4
- Gene
- StPUB17(V314I,V316I)e4
- Outcome
- CF4-mediated cell deathe4
- Direction
- suppressede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e4“Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization”
Claim · model-written summary
Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant enhanced P. infestans colonization
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutante4
- Interactor
- P. infestansPhytophthora infestanse4pathogen
- Gene
- StPUB17(V314I,V316I)e4
- Outcome
- P. infestans colonizatione4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e4“Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization”
Claim · model-written summary
PUB17 localizes to the plant nucleus and especially to the nucleolus
Verbatim
e5“PUB17 localizes to the plant nucleus and especially in the nucleolus”
Claim · model-written summary
Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Exclusion of the StPUB17(V314I,V316I) mutant from the nucleuse6
- Gene
- StPUB17(V314I,V316I)e6
- Outcome
- dominant-negative activitye6
- Direction
- abolishede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here”
e6“Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity”
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