Abstract
The potato blight agent Phytophthora infestans secretes a range of RXLR effectors to promote disease. Recent evidence indicates that some effectors suppress early pattern-triggered immunity (PTI) following perception of microbe-associated molecular patterns (MAMPs). Phytophthora infestans effector PiSFI3/Pi06087/PexRD16 has been previously shown to suppress MAMP-triggered pFRK1-Luciferase reporter gene activity. How PiSFI3 suppresses immunity is unknown. We employed yeast-two-hybrid (Y2H) assays, co-immunoprecipitation, transcriptional silencing by RNA interference and virus-induced gene silencing (VIGS), and X-ray crystallography for structure-guided mutagenesis, to investigate the function of PiSFI3 in targeting a plant U-box-kinase protein (StUBK) to suppress immunity. We discovered that PiSFI3 is active in the host nucleus and interacts in yeast and in planta with StUBK. UBK is a positive regulator of specific PTI pathways in both potato and Nicotiana benthamiana. Importantly, it contributes to early transcriptional responses that are suppressed by PiSFI3. PiSFI3 forms an unusual trans-homodimer. Mutation to disrupt dimerization prevents nucleolar localisation of PiSFI3 and attenuates both its interaction with StUBK and its ability to enhance P. infestans leaf colonisation. PiSFI3 is a 'WY-domain' RXLR effector that forms a novel trans-homodimer which is required for its ability to suppress PTI via interaction with the U-box-kinase protein StUBK.
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Claims 9
Claim · model-written summary
PiSFI3 was active in the host nucleus and interacted with StUBK in yeast and plants.
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- PiSFI3e0pathogen
- Gene
- StUBKe0
- Outcome
- interacts in yeast and in planta with StUBKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PiSFI3 is active in the host nucleus and interacts in yeast and in planta with StUBK”
t0“potato”
Claim · model-written summary
UBK positively regulated specific PTI pathways in potato.
- Taxon
- potatoSolanum tuberosume0
- Gene
- UBKe0
- Outcome
- specific PTI pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBK is a positive regulator of specific PTI pathways in both potato and Nicotiana benthamiana”
Claim · model-written summary
UBK positively regulated specific PTI pathways in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- UBKe0
- Outcome
- specific PTI pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBK is a positive regulator of specific PTI pathways in both potato and Nicotiana benthamiana”
Claim · model-written summary
UBK contributed to early transcriptional responses suppressed by PiSFI3.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- PiSFI3e0pathogen
- Gene
- UBKe1
- Outcome
- early transcriptional responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it contributes to early transcriptional responses that are suppressed by PiSFI3”
e1“UBK is a positive regulator of specific PTI pathways in both potato and Nicotiana benthamiana”
Claim · model-written summary
PiSFI3 formed an unusual trans-homodimer.
- Taxon
- Phytophthora infestans
Phytophthora infestanst0 - Gene
- PiSFI3e0
- Outcome
- unusual trans-homodimere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PiSFI3 forms an unusual trans-homodimer”
t0“Phytophthora infestans”
Claim · model-written summary
Disrupting dimerization prevented PiSFI3 nucleolar localization.
- Taxon
- Phytophthora infestansPhytophthora infestanst0
- Treatment
- Mutation to disrupt dimerizatione0
- Outcome
- nucleolar localisation of PiSFI3e0
- Direction
- preventse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation to disrupt dimerization prevents nucleolar localisation of PiSFI3”
t0“Phytophthora infestans”
Claim · model-written summary
Disrupting dimerization attenuated PiSFI3 interaction with StUBK.
- Taxon
- Phytophthora infestansPhytophthora infestanst0
- Treatment
- Mutation to disrupt dimerizatione0
- Outcome
- interaction with StUBKe0
- Direction
- attenuatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation to disrupt dimerization prevents nucleolar localisation of PiSFI3 and attenuates both its interaction with StUBK”
t0“Phytophthora infestans”
Claim · model-written summary
Disrupting PiSFI3 dimerization attenuated its ability to enhance P. infestans leaf colonization.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Mutation to disrupt dimerizatione0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- P. infestans leaf colonisatione0
- Direction
- attenuatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation to disrupt dimerization prevents nucleolar localisation of PiSFI3 and attenuates both its interaction with StUBK and its ability to enhance P. infestans leaf colonisation”
t0“potato”
Claim · model-written summary
The authors infer that PiSFI3 dimerization is required to suppress PTI through StUBK interaction.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StUBKe0
- Outcome
- suppress PTIe0
- Qualifier
- novel trans-homodimere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PiSFI3 is a 'WY-domain' RXLR effector that forms a novel trans-homodimer which is required for its ability to suppress PTI via interaction with the U-box-kinase protein StUBK”
t0“potato”
The same questions in other papers
loss of function · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
15 papers in the same direction as this paper · 15 in the opposite direction
- no effect
- no effect
- no effect
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.