Abstract
Adventitious rooting is an essential but sometimes rate-limiting step in the clonal multiplication of elite tree germplasm, because the ability to form roots declines rapidly with age in mature adult plant tissues. In spite of the importance of adventitious rooting, the mechanism behind this developmental process remains poorly understood. We have described the transcriptional profiles that are associated with the developmental stages of adventitious root formation in the model tree poplar (Populus trichocarpa). Transcriptome analyses indicate a highly specific temporal induction of the AINTEGUMENTA LIKE1 (PtAIL1) transcription factor of the AP2 family during adventitious root formation. Transgenic poplar samples that overexpressed PtAIL1 were able to grow an increased number of adventitious roots, whereas RNA interference mediated the down-expression of PtAIL1 expression, which led to a delay in adventitious root formation. Microarray analysis showed that the expression of 15 genes, including the transcription factors AGAMOUS-Like6 and MYB36, was overexpressed in the stem tissues that generated root primordia in PtAIL1-overexpressing plants, whereas their expression was reduced in the RNA interference lines. These results demonstrate that PtAIL1 is a positive regulator of poplar rooting that acts early in the development of adventitious roots.
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Claims 6
Claim · model-written summary
PtAIL1 was temporally induced during adventitious root formation.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“poplar (Populus trichocarpa). Transcriptome analyses indicate a highly specific temporal induction of the AINTEGUMENTA LIKE1 (PtAIL1) transcription factor of the AP2 family during adventitious root formation”
Claim · model-written summary
PtAIL1 overexpression increased adventitious root number.
- Treatment
- overexpressed PtAIL1e0
- Gene
- PtAIL1e0
- Outcome
- number of adventitious rootse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic poplar samples that overexpressed PtAIL1 were able to grow an increased number of adventitious roots”
Claim · model-written summary
PtAIL1 RNA interference delayed adventitious root formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“poplar samples that overexpressed PtAIL1 were able to grow an increased number of adventitious roots, whereas RNA interference mediated the down-expression of PtAIL1 expression, which led to a delay in adventitious root formation”
Claim · model-written summary
Fifteen genes were overexpressed in root-primordium-forming stem tissue of PtAIL1-overexpressing plants.
- Material
- PtAIL1-overexpressing plantse0
- Organ / stage
- stem tissuese0
- Gene
- AGAMOUS-Like6 and MYB36e0
- Outcome
- expression of 15 genese0
- Direction
- overexpressede0
- Value
- 15 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“poplar samples that overexpressed PtAIL1 were able to grow an increased number of adventitious roots, whereas RNA interference mediated the down-expression of PtAIL1 expression, which led to a delay in adventitious root formation. Microarray analysis showed that the expression of 15 genes, including the transcription factors AGAMOUS-Like6 and MYB36, was overexpressed in the stem tissues that generated root primordia in PtAIL1-overexpressing plants”
Claim · model-written summary
Expression of these genes was reduced in RNA interference lines.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“poplar samples that overexpressed PtAIL1 were able to grow an increased number of adventitious roots, whereas RNA interference mediated the down-expression of PtAIL1 expression, which led to a delay in adventitious root formation. Microarray analysis showed that the expression of 15 genes, including the transcription factors AGAMOUS-Like6 and MYB36, was overexpressed in the stem tissues that generated root primordia in PtAIL1-overexpressing plants, whereas their expression was reduced in the RNA interference lines”
Claim · model-written summary
PtAIL1 acts early as a positive regulator of poplar rooting.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtAIL1 is a positive regulator of poplar rooting that acts early in the development of adventitious roots”
The same questions in other papers
loss of function · growth · Populus (poplar)Positive and negative results
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.