Abstract
Exogenously applied jasmonic acid (JA) was used to study changes in protein patterns in rice (Oryza sativa L.) seedling tissues, to classify these changes, and to assign a role for these changes, in order to define the role of JA in the rice self-defense mechanism. High resolution two-dimensional polyacrylamide gel electrophoretic analysis revealed induction of new proteins in both leaf and stem tissues after JA treatment, with the major protein spots further analyzed through N-terminal and internal amino acid sequencing, purification, antibody production, and immunoblot analysis. JA treatment results in necrosis in these tissues, which is accompanied by drastic reductions in ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) subunits, and was confirmed using immunoblotting. Induction of novel proteins was found particularly in the stem tissues, including a new basic 28 kDa Bowman-Birk proteinase inhibitor protein (BBPIN; jasmonate-induced stem protein, JISP 6), and acidic 17 kDa pathogenesis-related class 1 protein (PR-1, JISP 9). This induction of proteins was blocked by a protein synthesis inhibitor cycloheximide (CHX) indicating de novo protein synthesis. Kinetin (KIN), a cytokinin and free radical scavenger reversed RuBisCO decreases, but not induction of proteins. Immunoblot analysis using antibodies generated against these purified proteins revealed a tissue-specific expression pattern and time-dependent induction after JA treatment. Our results indicate that jasmonate affects defense-related gene expression in rice seedlings, as evidenced by de novo synthesis of novel proteins with potential roles in plant defense.
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Claims 10
Claim · model-written summary
JA treatment induced new leaf proteins.
- Taxon
- rice (Oryza sativa L.)
Oryza sativat0 - Organ / stage
- leafe0
- Treatment
- JA treatmente0
- Outcome
- new proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of new proteins in both leaf and stem tissues after JA treatment”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JA treatment induced new stem proteins.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Organ / stage
- steme0
- Treatment
- JA treatmente0
- Outcome
- new proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of new proteins in both leaf and stem tissues after JA treatment”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JA treatment produced necrosis in rice seedling tissues.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- JA treatmente0
- Outcome
- necrosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA treatment results in necrosis in these tissues”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JA treatment reduced RuBisCO subunits.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- JA treatmente0
- Gene
- ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)e0
- Outcome
- ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) subunitse0
- Direction
- reductionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA treatment results in necrosis in these tissues, which is accompanied by drastic reductions in ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) subunits”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Stem tissues showed induction of BBPIN.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Organ / stage
- stem tissuese0
- Gene
- Bowman-Birk proteinase inhibitor protein (BBPIN; jasmonate-induced stem protein, JISP 6)e0
- Outcome
- Induction of novel proteinse0
- Value
- 28 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induction of novel proteins was found particularly in the stem tissues, including a new basic 28 kDa Bowman-Birk proteinase inhibitor protein (BBPIN; jasmonate-induced stem protein, JISP 6)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Stem tissues showed induction of PR-1.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Gene
- pathogenesis-related class 1 protein (PR-1, JISP 9)e0
- Outcome
- pathogenesis-related class 1 proteine0
- Value
- 17 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“acidic 17 kDa pathogenesis-related class 1 protein (PR-1, JISP 9)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Cycloheximide blocked JA-induced protein induction.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- cycloheximide (CHX)e0
- Outcome
- induction of proteinse0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This induction of proteins was blocked by a protein synthesis inhibitor cycloheximide (CHX)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Kinetin reversed RuBisCO decreases.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- Kinetin (KIN)e0
- Gene
- RuBisCOe0
- Outcome
- RuBisCO decreasese0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin (KIN), a cytokinin and free radical scavenger reversed RuBisCO decreases”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Kinetin did not reverse induction of proteins.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- Kinetin (KIN)e0
- Outcome
- induction of proteinse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin (KIN), a cytokinin and free radical scavenger reversed RuBisCO decreases, but not induction of proteins”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JA-induced proteins had tissue-specific and time-dependent expression.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- JA treatmente0
- Outcome
- tissue-specific expression pattern and time-dependent inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tissue-specific expression pattern and time-dependent induction after JA treatment”
t0“rice (Oryza sativa L.)”
The same questions in other papers
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3 papers in the same direction as this paper
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.