Abstract
cis-natural antisense transcripts (cis-NATs) are widespread in plants and are often associated with downregulation of their associated sense genes. We found that a cis-NAT positively regulates the level of a protein critical for phosphate homeostasis in rice (Oryza sativa). PHOSPHATE1;2 (PHO1;2), a gene involved in phosphate loading into the xylem in rice, and its associated cis-NATPHO1;2 are both controlled by promoters active in the vascular cylinder of roots and leaves. While the PHO1;2 promoter is unresponsive to the plant phosphate status, the cis-NATPHO1;2 promoter is strongly upregulated under phosphate deficiency. Expression of both cis-NATPHO1;2 and the PHO1;2 protein increased in phosphate-deficient plants, while the PHO1;2 mRNA level remained stable. Downregulation of cis-NATPHO1;2 expression by RNA interference resulted in a decrease in PHO1;2 protein, impaired the transfer of phosphate from root to shoot, and decreased seed yield. Constitutive overexpression of NATPHO1;2 in trans led to a strong increase of PHO1;2, even under phosphate-sufficient conditions. Under all conditions, no changes occurred in the level of expression, sequence, or nuclear export of PHO1;2 mRNA. However, expression of cis-NATPHO1;2 was associated with a shift of both PHO1;2 and cis-NATPHO1;2 toward the polysomes. These findings reveal an unexpected role for cis-NATPHO1;2 in promoting PHO1;2 translation and affecting phosphate homeostasis and plant fitness.
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Claims 10
Claim · model-written summary
PHO1;2 and cis-NATPHO1;2 promoters were active in the vascular cylinder of roots and leaves.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- vascular cylinder of roots and leavese0
- Gene
- PHO1;2e0
- Outcome
- promoters active in the vascular cylinder of roots and leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PHOSPHATE1;2 (PHO1;2), a gene involved in phosphate loading into the xylem in rice, and its associated cis-NATPHO1;2 are both controlled by promoters active in the vascular cylinder of roots and leaves”
Claim · model-written summary
The PHO1;2 promoter was unresponsive to plant phosphate status.
- Taxon
- riceOryza sativae1
- Gene
- PHO1;2e0
- Outcome
- PHO1;2 promotere0
- Direction
- unresponsivee0
- Qualifier
- plant phosphate statuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PHO1;2 promoter is unresponsive to the plant phosphate status”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
The cis-NATPHO1;2 promoter was strongly upregulated during phosphate deficiency.
- Taxon
- riceOryza sativae1
- Treatment
- phosphate deficiencye0
- Gene
- cis-NATPHO1;2e0
- Outcome
- cis-NATPHO1;2 promotere0
- Direction
- strongly upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cis-NATPHO1;2 promoter is strongly upregulated under phosphate deficiency”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
Phosphate-deficient plants increased cis-NATPHO1;2 expression and PHO1;2 protein while PHO1;2 mRNA stayed stable.
- Taxon
- riceOryza sativae1
- Treatment
- phosphate-deficiente0
- Gene
- cis-NATPHO1;2e0
- Outcome
- Expression of both cis-NATPHO1;2 and the PHO1;2 proteine0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of both cis-NATPHO1;2 and the PHO1;2 protein increased in phosphate-deficient plants, while the PHO1;2 mRNA level remained stable”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
RNA interference of cis-NATPHO1;2 decreased PHO1;2 protein.
- Taxon
- riceOryza sativae1
- Treatment
- Downregulation of cis-NATPHO1;2 expression by RNA interferencee0
- Gene
- cis-NATPHO1;2e0
- Outcome
- PHO1;2 proteine0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of cis-NATPHO1;2 expression by RNA interference resulted in a decrease in PHO1;2 protein”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
RNA interference of cis-NATPHO1;2 impaired root-to-shoot phosphate transfer.
- Taxon
- riceOryza sativae1
- Treatment
- Downregulation of cis-NATPHO1;2 expression by RNA interferencee0
- Gene
- cis-NATPHO1;2e0
- Outcome
- transfer of phosphate from root to shoote0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of cis-NATPHO1;2 expression by RNA interference resulted in a decrease in PHO1;2 protein, impaired the transfer of phosphate from root to shoot”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
RNA interference of cis-NATPHO1;2 decreased seed yield.
- Taxon
- riceOryza sativae1
- Treatment
- Downregulation of cis-NATPHO1;2 expression by RNA interferencee0
- Gene
- cis-NATPHO1;2e0
- Outcome
- seed yielde0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of cis-NATPHO1;2 expression by RNA interference resulted in a decrease in PHO1;2 protein, impaired the transfer of phosphate from root to shoot, and decreased seed yield”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
Constitutive NATPHO1;2 overexpression strongly increased PHO1;2 even under phosphate sufficiency.
- Taxon
- riceOryza sativae1
- Treatment
- Constitutive overexpression of NATPHO1;2 in transe0
- Gene
- NATPHO1;2e0
- Outcome
- PHO1;2e0
- Direction
- strong increasee0
- Qualifier
- even under phosphate-sufficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive overexpression of NATPHO1;2 in trans led to a strong increase of PHO1;2, even under phosphate-sufficient conditions”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
PHO1;2 mRNA expression, sequence, and nuclear export did not change under any condition.
- Taxon
- riceOryza sativae1
- Gene
- PHO1;2e0
- Outcome
- level of expression, sequence, or nuclear export of PHO1;2 mRNAe0
- Direction
- no changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under all conditions, no changes occurred in the level of expression, sequence, or nuclear export of PHO1;2 mRNA”
e1“homeostasis in rice (Oryza sativa)”
Claim · model-written summary
cis-NATPHO1;2 expression was associated with movement of both RNAs toward polysomes.
- Taxon
- riceOryza sativae1
- Gene
- cis-NATPHO1;2e0
- Outcome
- shift of both PHO1;2 and cis-NATPHO1;2 toward the polysomese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of cis-NATPHO1;2 was associated with a shift of both PHO1;2 and cis-NATPHO1;2 toward the polysomes”
e1“homeostasis in rice (Oryza sativa)”
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