Abstract
The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.
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Claims 6
Claim · model-written summary
StNOA1 integration was verified in four transgenic Arabidopsis lines.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- transgenic Atnoa1e0
- Outcome
- Transgene integratione0
- Value
- 4 different linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1.”
Claim · model-written summary
StNOA1 expression was detected by RT-PCR in transgenic Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- StNOA1e0
- Outcome
- Expression of StNOA1 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR.”
Claim · model-written summary
Under salt stress, TL9 had higher proline, soluble protein and chlorophyll contents than the Atnoa1 mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- transgenic line TL9e0
- Treatment
- salt stress conditione0
- Outcome
- proline, soluble protein and chlorophyll contentse0
- Direction
- highere0
- Comparator
- Atnoa1 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition.”
Claim · model-written summary
Under salt stress, TL9 had lower malondialdehyde content than the Atnoa1 mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- salt stress conditione0
- Outcome
- content of malondialdehyde (MDA)e0
- Direction
- lowere0
- Comparator
- Atnoa1 mutante0
Not reportedMaterial
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition.”
Claim · model-written summary
TL9 root elongation was significantly higher than Atnoa1 under salt stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- TL9e0
- Treatment
- salt stresse0
- Outcome
- Root elongatione0
- Direction
- highere0
- Comparator
- Atnoa1 seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress.”
Claim · model-written summary
TL9 survival rate was significantly higher than Atnoa1 under salt stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- TL9e0
- Treatment
- salt stresse0
- Outcome
- survival ratee0
- Direction
- highere0
- Comparator
- Atnoa1 seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress.”
The same questions in other papers
salinity · growth · Arabidopsis thaliana (thale cress)Positive and negative results
3 papers in the same direction as this paper · 11 in the opposite direction
- negative
- no effect
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.