Abstract
To better understand the role of active oxygen species (AOS) in acquired resistance to increased levels of ultraviolet (UV)-B irradiation in plants, we isolated an Arabidopsis mutant that is resistant to methyl viologen, and its sensitivity to UV-B was investigated. A complementation test revealed that the obtained mutant was allelic to the ozone-sensitive radical-induced cell death1-1 (rcd1-1). Therefore, this mutant was named rcd1-2. rcd1-2 was recessive and nearly 4-fold more resistant to methyl viologen than wild type. It exhibited a higher tolerance to short-term UV-B supplementation treatments than the wild type: UV-B-induced formation of cyclobutane pyrimidine dimers was reduced by one-half after 24 h of exposure; the decrease in quantum yield of photosystem II was also diminished by 40% after 12 h of treatment. Furthermore, rcd1-2 was tolerant to freezing. Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type, and the mRNA level of the latter enzyme was enhanced by UV-B exposure more effectively in rcd1-2. UV-B-absorbing compounds were more accumulated in rcd1-2 than in wild type after UV-B exposure for 24 h. These findings suggest that rcd1-2 methyl viologen resistance is due to the enhanced activities of the AOS-scavenging enzymes in chloroplasts and that the acquired tolerance to the short-term UV-B exposure results from a higher accumulation of sunscreen pigments. rcd1 appears to be a mutant that constitutively shows stress responses, leading to accumulation of more pigments and AOS-scavenging enzymes without any stresses.
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Claims 12
Claim · model-written summary
The rcd1-2 mutant was allelic to the ozone-sensitive rcd1-1.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- rcd1-1e1
- Outcome
- allelic to the ozone-sensitive radical-induced cell death1-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e1“A complementation test revealed that the obtained mutant was allelic to the ozone-sensitive radical-induced cell death1-1 (rcd1-1)”
Claim · model-written summary
The rcd1-2 mutation is recessive.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e2
- Outcome
- recessivee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e2“rcd1-2 was recessive and nearly 4-fold more resistant to methyl viologen than wild type”
Claim · model-written summary
The rcd1-2 mutant is nearly 4-fold more resistant to methyl viologen than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e2
- Treatment
- methyl viologene2
- Outcome
- resistant to methyl viologene2
- Direction
- moree2
- Value
- nearly 4-folde2
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e2“rcd1-2 was recessive and nearly 4-fold more resistant to methyl viologen than wild type”
Claim · model-written summary
Short-term UV-B supplementation reduced the formation of cyclobutane pyrimidine dimers by one-half in rcd1-2 relative to wild type after 24 h.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- short-term UV-B supplementatione3
- Outcome
- formation of cyclobutane pyrimidine dimerse3
- Direction
- reducede3
- Value
- one-halfe3
- Comparator
- wild typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e3“It exhibited a higher tolerance to short-term UV-B supplementation treatments than the wild type: UV-B-induced formation of cyclobutane pyrimidine dimers was reduced by one-half after 24 h of exposure”
Claim · model-written summary
Short-term UV-B supplementation diminished the decrease in quantum yield of photosystem II by 40% after 12 h in rcd1-2 relative to wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- short-term UV-B supplementatione3
- Outcome
- decrease in quantum yield of photosystem IIe4
- Direction
- diminishede4
- Value
- 40%e4
- Comparator
- wild typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e3“It exhibited a higher tolerance to short-term UV-B supplementation treatments than the wild type: UV-B-induced formation of cyclobutane pyrimidine dimers was reduced by one-half after 24 h of exposure”
e4“the decrease in quantum yield of photosystem II was also diminished by 40% after 12 h of treatment”
Claim · model-written summary
The rcd1-2 mutant is tolerant to freezing.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e5
- Outcome
- tolerant to freezinge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e5“rcd1-2 was tolerant to freezing”
Claim · model-written summary
Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e6
- Gene
- plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidasee6
- Outcome
- Steady-state mRNA levelse6
- Direction
- highere6
- Comparator
- wild typee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e6“Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type”
Claim · model-written summary
UV-B exposure enhanced the stromal ascorbate peroxidase mRNA level more effectively in rcd1-2.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- UV-B exposuree7
- Gene
- stromal ascorbate peroxidasee6
- Outcome
- mRNA levele7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e6“Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type”
e7“the mRNA level of the latter enzyme was enhanced by UV-B exposure more effectively in rcd1-2”
Claim · model-written summary
UV-B-absorbing compounds accumulated more in rcd1-2 than in wild type after 24 h of UV-B exposure.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e1
- Treatment
- UV-B exposuree1
- Outcome
- UV-B-absorbing compoundse1
- Direction
- more accumulatede1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e1“UV-B-absorbing compounds were more accumulated in rcd1-2 than in wild type after UV-B exposure for 24 h”
Claim · model-written summary
Methyl viologen resistance of rcd1-2 is due to enhanced activities of AOS-scavenging enzymes in chloroplasts.
- Taxon
- ArabidopsisArabidopsise0
- Material
- rcd1-2e1
- Outcome
- methyl viologen resistancee1
- Qualifier
- enhanced activities of the AOS-scavenging enzymes in chloroplastse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e1“rcd1-2 methyl viologen resistance is due to the enhanced activities of the AOS-scavenging enzymes in chloroplasts”
Claim · model-written summary
The acquired tolerance to short-term UV-B exposure results from a higher accumulation of sunscreen pigments.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- tolerance to the short-term UV-B exposuree1
- Qualifier
- a higher accumulation of sunscreen pigmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e1“the acquired tolerance to the short-term UV-B exposure results from a higher accumulation of sunscreen pigments”
Claim · model-written summary
rcd1 appears to be a mutant that constitutively shows stress responses, with more pigments and AOS-scavenging enzymes accumulating without stress.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- stress responsese1
- Qualifier
- accumulation of more pigments and AOS-scavenging enzymes without any stressese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an Arabidopsis mutant that is resistant to methyl viologen”
e1“rcd1 appears to be a mutant that constitutively shows stress responses, leading to accumulation of more pigments and AOS-scavenging enzymes without any stresses”
The same questions in other papers
uv · molecular: expression · ArabidopsisPositive and negative results
11 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- no effect
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.