Abstract
In the germinated cereal aleurone layer, gibberellic acids (GA) induce expression of a number of genes encoding hydrolytic enzymes that participate in the mobilization of stored molecules. Previous analyses suggest that the key events controlling the GA-regulated gene expression in the aleurone are formation of active transcription machinery referred to as the GA responsive complex, followed by recruiting GAMYB. In general, bipartite promoter contexts composed of the GA-responsive element and the pyrimidine box are observed within the regulatory regions of cereal GA-responsive genes. Protein factors that recognize each promoter sequence were identified and distinct effects on the GA-mediated activation of gene expression have been also investigated; however, the connection and intercalation between two promoter motifs remain obscure. In this study, I have evaluated cooperative function of GAMYB and a pyrimidine box-binding protein OsDOF3 that influenced the promoter activity of the most predominant GA-responsive gene (RAmy1A) of rice (Oryza sativa). Transient expression of OsDOF3 in the germinated aleurone prolonged GAMYB function on the reporter expression in the absence of GA. The synergistic effect required a set of DNA bindings of two proteins on the RAmy1A promoter region. The yeast two-hybrid assay showed the physical interaction of GAMYB and OsDOF3 in yeast cells, indicating that the association of GAMYB and OsDOF3 may be a functional unit in transcription regulation. The results showed the accessory function of OsDOF3 responsible for a dosage-dependent mediation of GA signaling that leads to high-level expression of physiological target genes.
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Claims 4
Claim · model-written summary
OsDOF3 expression prolonged GAMYB driven reporter expression without GA.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- germinated aleuronee0
- Treatment
- Transient expression of OsDOF3e0
- Gene
- OsDOF3e0
- Outcome
- reporter expressione0
- Direction
- prolongede0
- Qualifier
- absence of GAe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Aleurone”
e0“Transient expression of OsDOF3 in the germinated aleurone prolonged GAMYB function on the reporter expression in the absence of GA.”
Claim · model-written summary
The synergistic effect required binding of both proteins at the RAmy1A promoter.
- Taxon
- RiceOryza sativat0
- Gene
- RAmy1Ae0
- Outcome
- synergistic effecte0
- Qualifier
- DNA bindings of two proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Aleurone”
e0“The synergistic effect required a set of DNA bindings of two proteins on the RAmy1A promoter region.”
Claim · model-written summary
GAMYB and OsDOF3 physically interacted in yeast cells.
- Taxon
- RiceOryza sativat0
- Gene
- GAMYB and OsDOF3e0
- Outcome
- physical interaction of GAMYB and OsDOF3e0
- Setting
- yeast cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Aleurone”
e0“The yeast two-hybrid assay showed the physical interaction of GAMYB and OsDOF3 in yeast cells”
Claim · model-written summary
OsDOF3 mediated GA signalling in a dosage dependent manner, leading to high target gene expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsDOF3e0
- Outcome
- high-level expression of physiological target genese0
- Qualifier
- dosage-dependent mediation of GA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Aleurone”
e0“accessory function of OsDOF3 responsible for a dosage-dependent mediation of GA signaling that leads to high-level expression of physiological target genes.”
The same questions in other papers
transgenic · molecular: expression · Oryza sativa (rice)Positive and negative results
14 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
24 papers more in this group
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