Abstract
The molecular mechanism by which GA regulates plant growth and development has been a subject of active research. Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes, among which OsWRKY71 is highly expressed in aleurone cells. Transient expression of OsWRKY71 by particle bombardment specifically represses GA-induced Amy32b alpha-amylase promoter but not abscisic acid-induced HVA22 or HVA1 promoter activity in aleurone cells. Moreover, OsWRKY71 blocks the activation of the Amy32b promoter by the GA-inducible transcriptional activator OsGAMYB. Consistent with its role as a transcriptional repressor, OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally defined TGAC-containing W boxes of the Amy32b promoter in vitro. Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71, suggesting that OsWRKY71 blocks GA signaling by functionally interfering with OsGAMYB. Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 and destabilizes the GFP:OsWRKY71 fusion protein. These findings suggest that OsWRKY71 encodes a transcriptional repressor of GA signaling in aleurone cells.
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Claims 13
Claim · model-written summary
Analysis of rice genomic sequences identified 77 WRKY genes.
- Taxon
- rice (Oryza sativa)
Oryza sativae0 - Outcome
- WRKY genese0
- Value
- 77e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes, among which OsWRKY71 is highly expressed in aleurone cells”
Claim · model-written summary
OsWRKY71 is highly expressed in rice aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Organ / stage
- aleurone cellse0
- Gene
- OsWRKY71e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes, among which OsWRKY71 is highly expressed in aleurone cells”
Claim · model-written summary
Transient expression of OsWRKY71 represses GA-induced Amy32b alpha-amylase promoter activity in aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Organ / stage
- aleurone cellse1
- Treatment
- Transient expression of OsWRKY71 by particle bombardmente1
- Gene
- Amy32be1
- Outcome
- GA-induced Amy32b alpha-amylase promotere1
- Direction
- repressese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e1“Transient expression of OsWRKY71 by particle bombardment specifically represses GA-induced Amy32b alpha-amylase promoter but not abscisic acid-induced HVA22 or HVA1 promoter activity in aleurone cells”
Claim · model-written summary
Transient expression of OsWRKY71 does not repress abscisic acid-induced HVA22 or HVA1 promoter activity in aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- Transient expression of OsWRKY71 by particle bombardmente1
- Gene
- OsWRKY71e1
- Outcome
- abscisic acid-induced HVA22 or HVA1 promoter activitye1
- Direction
- but note1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e1“Transient expression of OsWRKY71 by particle bombardment specifically represses GA-induced Amy32b alpha-amylase promoter but not abscisic acid-induced HVA22 or HVA1 promoter activity in aleurone cells”
Claim · model-written summary
OsWRKY71 blocks the activation of the Amy32b promoter by the GA-inducible activator OsGAMYB.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- OsWRKY71e2
- Gene
- OsGAMYBe2
- Outcome
- activation of the Amy32b promotere2
- Direction
- blockse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e2“OsWRKY71 blocks the activation of the Amy32b promoter by the GA-inducible transcriptional activator OsGAMYB”
Claim · model-written summary
OsWRKY71 is localized to nuclei of rice aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Organ / stage
- aleurone cellse3
- Gene
- OsWRKY71e3
- Outcome
- localized to nuclei of aleurone cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e3“OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally defined TGAC-containing W boxes of the Amy32b promoter in vitro”
Claim · model-written summary
OsWRKY71 binds specifically to TGAC-containing W boxes of the Amy32b promoter in vitro.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Gene
- OsWRKY71e3
- Outcome
- binds specifically to functionally defined TGAC-containing W boxes of the Amy32b promotere3
- Setting
- in vitroe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e3“OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally defined TGAC-containing W boxes of the Amy32b promoter in vitro”
Claim · model-written summary
Mutation of the two W boxes prevents binding of OsWRKY71 to the Amy32b promoter.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- Mutation of the two W boxese4
- Gene
- OsWRKY71e4
- Outcome
- binding of OsWRKY71e4
- Direction
- preventse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e4“Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71”
Claim · model-written summary
Mutation of the two W boxes releases the suppression of OsGAMYB-activated Amy32b expression by OsWRKY71.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- Mutation of the two W boxese4
- Gene
- OsGAMYBe4
- Outcome
- suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71e4
- Direction
- releasese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e4“Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71”
Claim · model-written summary
OsWRKY71 is suggested to block GA signalling by functionally interfering with OsGAMYB.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Gene
- OsGAMYBe5
- Outcome
- GA signalinge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e5“suggesting that OsWRKY71 blocks GA signaling by functionally interfering with OsGAMYB”
Claim · model-written summary
Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 in rice aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- Exogenous GA treatmente6
- Gene
- OsWRKY71e6
- Outcome
- steady-state mRNA level of OsWRKY71e6
- Direction
- decreasese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e6“Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 and destabilizes the GFP:OsWRKY71 fusion protein”
Claim · model-written summary
Exogenous GA treatment destabilizes the GFP:OsWRKY71 fusion protein.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Treatment
- Exogenous GA treatmente6
- Gene
- GFP:OsWRKY71e6
- Outcome
- GFP:OsWRKY71 fusion proteine6
- Direction
- destabilizese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e6“Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 and destabilizes the GFP:OsWRKY71 fusion protein”
Claim · model-written summary
OsWRKY71 is suggested to encode a transcriptional repressor of GA signalling in aleurone cells.
- Taxon
- rice (Oryza sativa)Oryza sativae0
- Gene
- OsWRKY71e7
- Outcome
- GA signaling in aleurone cellse7
- Qualifier
- transcriptional repressore7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes”
e7“These findings suggest that OsWRKY71 encodes a transcriptional repressor of GA signaling in aleurone cells”
The same questions in other papers
transgenic · molecular: expression · Oryza sativa (rice)Positive and negative results
12 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.