Abstract
Four cDNA clones named CPRD (cowpea responsive to dehydration) corresponding to genes that are responsive to dehydration were isolated using differential screening of a cDNA library prepared from 10-h dehydrated drought-tolerant cowpea (Vigna unguiculata) plants. One of the cDNA clones has a homology to 9-cis-epoxycarotenoid dioxygenase (named VuNCED1), which is supposed to be involved in abscisic acid (ABA) biosynthesis. The GST (glutathione S-transferase)-fused protein indicates a 9-cis-epoxycarotenoid dioxygenase activity, which catalyzes the cleavage of 9-cis-epoxycarotenoid. The N-terminal region of the VuNCED1 protein directed the fused sGFP (synthetic green-fluorescent protein) into the plastids of the protoplasts, indicating that the N-terminal sequence acts as a transit peptide. Both the accumulation of ABA and expression of VuNCED1 were strongly induced by drought stress in the 8-d-old cowpea plant, whereas drought stress did not trigger the expression of VuABA1 (accession no. AB030295) gene that encodes zeaxanthin epoxidase. These results indicate that the VuNCED1 cDNA encodes a 9-cis-epoxycarotenoid dioxygenase and that its product has a key role in the synthesis of ABA under drought stress.
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Claims 8
Claim · model-written summary
Four dehydration responsive cowpea cDNA clones were isolated.
- Taxon
- cowpea
Vigna unguiculatae0 - Outcome
- cDNA clonese0
- Value
- Foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four cDNA clones named CPRD (cowpea responsive to dehydration) corresponding to genes that are responsive to dehydration were isolated”
Claim · model-written summary
VuNCED1 has homology to 9-cis-epoxycarotenoid dioxygenase.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- VuNCED1e0
- Outcome
- homology to 9-cis-epoxycarotenoid dioxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the cDNA clones has a homology to 9-cis-epoxycarotenoid dioxygenase (named VuNCED1)”
t0“Cowpea”
Claim · model-written summary
The fused protein showed 9-cis-epoxycarotenoid dioxygenase activity.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- GST (glutathione S-transferase)-fused proteine0
- Outcome
- 9-cis-epoxycarotenoid dioxygenase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GST (glutathione S-transferase)-fused protein indicates a 9-cis-epoxycarotenoid dioxygenase activity”
t0“Cowpea”
Claim · model-written summary
The VuNCED1 N terminal region directed fused sGFP to plastids.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- VuNCED1 proteine0
- Outcome
- into the plastids of the protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The N-terminal region of the VuNCED1 protein directed the fused sGFP (synthetic green-fluorescent protein) into the plastids of the protoplasts”
t0“Cowpea”
Claim · model-written summary
Drought strongly induced ABA accumulation in eight day old cowpea.
- Taxon
- cowpeaVigna unguiculatae0
- Treatment
- drought stresse0
- Outcome
- accumulation of ABAe0
- Direction
- strongly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the accumulation of ABA and expression of VuNCED1 were strongly induced by drought stress in the 8-d-old cowpea plant”
Claim · model-written summary
Drought strongly induced VuNCED1 expression.
- Taxon
- cowpeaVigna unguiculatae0
- Treatment
- drought stresse0
- Gene
- VuNCED1e0
- Outcome
- expression of VuNCED1e0
- Direction
- strongly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of VuNCED1 were strongly induced by drought stress in the 8-d-old cowpea plant”
Claim · model-written summary
Drought did not trigger VuABA1 expression.
- Taxon
- cowpeaVigna unguiculatat0
- Treatment
- drought stresse0
- Gene
- VuABA1e0
- Outcome
- expression of VuABA1e0
- Direction
- did not triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress did not trigger the expression of VuABA1 (accession no. AB030295) gene”
t0“Cowpea”
Claim · model-written summary
The authors infer that VuNCED1 product has a key role in ABA synthesis under drought.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- VuNCED1e2
- Outcome
- synthesis of ABAe0
- Qualifier
- under drought stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its product has a key role in the synthesis of ABA under drought stress.”
t0“Cowpea”
e2“One of the cDNA clones has a homology to 9-cis-epoxycarotenoid dioxygenase (named VuNCED1)”
The same questions in other papers
drought · molecular: expression · Vigna unguiculata (cowpea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
drought · molecular: metabolites · Vigna unguiculata (cowpea)Positive and negative results
5 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.