Abstract
A gene encoding putative β-carbonic anhydrase gene (CA) from cowpea (Vigna unguiculata L. Walp.) was characterized and designated as VuCA1 (GenBank ID: JQ429799). While the genomic sequence of CA was found to be 1470 bp long with 4 introns, the open reading frame was 990 bp in length. The deduced amino acid sequence of CA contained two characteristic conserved domains, i.e. CSDSRV and EYAVLHLKVSNIVVIGHSACG, which showed high degree of homology with other β-CA genes of angiosperms. We have reported three-dimensional model of VuCA1 and quality of the predicted model was analysed with PROCHECK. Molecular docking of modeled VuCA1 revealed similar binding pockets for different substrates and products. The expression of VuCA1 transcripts was detected in different parts of the cowpea plant, with highest level observed in leaves, followed by flower buds, weak expression in stems, and the weakest in roots. The expression of VuCA1 transcripts of leaves was downregulated by zinc deficiency but upregulated by salt and dehydration stress.
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Claims 10
Claim · model-written summary
VuCA1 was characterized as a putative carbonic anhydrase gene.
- Taxon
- cowpea
Vigna unguiculatae0 - Gene
- VuCA1e0
- Outcome
- was characterized and designated as VuCA1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene encoding putative β-carbonic anhydrase gene (CA) from cowpea (Vigna unguiculata L. Walp.) was characterized and designated as VuCA1 (GenBank ID: JQ429799).”
Claim · model-written summary
The CA genomic sequence was 1470 bp with four introns.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- CAe0
- Outcome
- genomic sequence of CAe0
- Value
- 1470 bpe0
- Qualifier
- 4 intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genomic sequence of CA was found to be 1470 bp long with 4 introns”
e1“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
The CA open reading frame was 990 bp.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- CAe2
- Outcome
- open reading framee0
- Value
- 990 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the open reading frame was 990 bp in length”
e1“from cowpea (Vigna unguiculata L. Walp.)”
e2“the genomic sequence of CA”
Claim · model-written summary
The deduced CA sequence contained two conserved domains homologous to angiosperm β-CA genes.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- CAe0
- Outcome
- two characteristic conserved domains, i.e. CSDSRV and EYAVLHLKVSNIVVIGHSACGe0
- Qualifier
- high degree of homology with other β-CA genes of angiospermse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequence of CA contained two characteristic conserved domains, i.e. CSDSRV and EYAVLHLKVSNIVVIGHSACG, which showed high degree of homology with other β-CA genes of angiosperms.”
e1“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
A three-dimensional model of VuCA1 was reported.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- VuCA1e0
- Outcome
- three-dimensional model of VuCA1e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have reported three-dimensional model of VuCA1 and quality of the predicted model was analysed with PROCHECK.”
e1“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
Docking found similar binding pockets for different substrates and products.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- VuCA1e0
- Outcome
- similar binding pockets for different substrates and productse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular docking of modeled VuCA1 revealed similar binding pockets for different substrates and products.”
e1“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
VuCA1 expression was highest in leaves, then flower buds, stems and roots.
- Taxon
- cowpeaVigna unguiculatae0
- Organ / stage
- leavese0
- Gene
- VuCA1e0
- Outcome
- expression of VuCA1 transcriptse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of VuCA1 transcripts was detected in different parts of the cowpea plant, with highest level observed in leaves, followed by flower buds, weak expression in stems, and the weakest in roots.”
Claim · model-written summary
Zinc deficiency downregulated leaf VuCA1 transcripts.
- Taxon
- cowpeaVigna unguiculatae1
- Organ / stage
- leavese0
- Treatment
- zinc deficiencye0
- Gene
- VuCA1e0
- Outcome
- expression of VuCA1 transcriptse0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of VuCA1 transcripts of leaves was downregulated by zinc deficiency”
e1“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
Salt stress upregulated leaf VuCA1 transcripts.
- Taxon
- cowpeaVigna unguiculatae2
- Treatment
- salte0
- Gene
- VuCA1e1
- Outcome
- expression of VuCA1 transcriptse1
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upregulated by salt and dehydration stress”
e1“The expression of VuCA1 transcripts of leaves”
e2“from cowpea (Vigna unguiculata L. Walp.)”
Claim · model-written summary
Dehydration stress upregulated leaf VuCA1 transcripts.
- Taxon
- cowpeaVigna unguiculatae2
- Treatment
- dehydration stresse0
- Gene
- VuCA1e1
- Outcome
- expression of VuCA1 transcriptse1
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upregulated by salt and dehydration stress”
e1“The expression of VuCA1 transcripts of leaves”
e2“from cowpea (Vigna unguiculata L. Walp.)”
The same questions in other papers
drought · molecular: expression · Vigna unguiculata (cowpea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
salinity · molecular: expression · Vigna unguiculata (cowpea)Positive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.