Abstract
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His(6) -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant immunity responses and a target for pathogen suppression.
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Claims 7
Claim · model-written summary
HopN1 suppressed defense-associated ROS and callose production in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- HopN1e0
- Interactor
- Pseudomonas fluorescens
.JPG?width=60)
Pseudomonas fluorescense0other - Gene
- HopN1e0
- Outcome
- defence-associated reactive oxygen species (ROS) and callosee0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS.”
Claim · model-written summary
Tomato PsbQ interacted with HopN1.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- PsbQe0
- Outcome
- interacting proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purified His(6) -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein.”
Claim · model-written summary
HopN1 localized to chloroplasts.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- chloroplastse0
- Gene
- HopN1e0
- Outcome
- localized to chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities.”
t0“tomato PsbQ, a member of the oxygen evolving complex”
Claim · model-written summary
HopN1 degraded PsbQ in chloroplast preparations.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- chloroplast preparationse0
- Treatment
- HopN1e0
- Gene
- PsbQe0
- Outcome
- PsbQe0
- Direction
- degradede0
- Comparator
- HopN1(D299A) non-catalytic mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities.”
t0“tomato PsbQ, a member of the oxygen evolving complex”
Claim · model-written summary
HopN1 inhibited PSII activity in chloroplast preparations.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- chloroplast preparationse0
- Treatment
- HopN1e0
- Outcome
- PSII activitye0
- Direction
- inhibitede0
- Comparator
- HopN1(D299A) non-catalytic mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities.”
t0“tomato PsbQ, a member of the oxygen evolving complex”
Claim · model-written summary
Silencing NtPsbQ compromised tobacco ROS production after DC3000 challenge.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Gene silencing of NtPsbQe0
- Interactor
- DC3000e0pathogen
- Gene
- NtPsbQe0
- Outcome
- ROS productione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000.”
Claim · model-written summary
Silencing NtPsbQ compromised tobacco programmed cell death after DC3000 challenge.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Gene silencing of NtPsbQe0
- Interactor
- DC3000e0pathogen
- Gene
- NtPsbQe0
- Outcome
- programmed cell deathe0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000.”
The same questions in other papers
loss of function · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
3 papers more in this group
See all 7 papers →loss of function · cytology · Nicotiana tabacum (tobacco)Results agree
8 papers in the same direction as this paper
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.