Abstract
In plants, the role of mitogen-activated protein kinase (MAPK) in reactive oxygen species (ROS)-based signal transduction processes is elusive. Despite the fact that ROS can induce MAPK activation, no direct genetic evidence has linked ROS-induced MAPK activation with the hypersensitive response, a form of programmed cell death. In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK). We found through gain-of-function and loss-of-function approaches that both overexpression and RNA interference-based suppression of SIPK render the plant sensitive to ROS stress. Transgenic lines overexpressing a nonphosphorylatable version of SIPK were not ROS sensitive. Analysis of the MAPK activation profiles in ROS-stressed transgenic and wild-type plants revealed a striking interplay between SIPK and another MAPK (wound-induced protein kinase [WIPK]) in the different kinotypes. During continuous ozone exposure, abnormally prolonged activation of SIPK was seen in the SIPK-overexpression genotype, without WIPK activation, whereas strong and stable activation of WIPK was observed in the SIPK-suppressed lines. Thus, one role of activated SIPK in tobacco cells upon ROS stimulation appears to be control of the inactivation of WIPK.
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Claims 8
Claim · model-written summary
SIPK is the major ROS-induced MAPK in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- salicylate-induced protein kinase (SIPK)e0
- Outcome
- major ROS-induced MAPKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
SIPK overexpression rendered tobacco plants ROS sensitive.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpressione0
- Gene
- SIPKe0
- Outcome
- sensitive to ROS stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both overexpression and RNA interference-based suppression of SIPK render the plant sensitive to ROS stress”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
RNA interference suppression of SIPK rendered tobacco plants ROS sensitive.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- RNA interference-based suppression of SIPKe0
- Gene
- SIPKe0
- Outcome
- sensitive to ROS stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both overexpression and RNA interference-based suppression of SIPK render the plant sensitive to ROS stress”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
Nonphosphorylatable SIPK overexpression did not confer ROS sensitivity.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpressing a nonphosphorylatable version of SIPKe0
- Gene
- SIPKe0
- Outcome
- not ROS sensitivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic lines overexpressing a nonphosphorylatable version of SIPK were not ROS sensitive.”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
Continuous ozone prolonged SIPK activation in SIPK-overexpressing plants.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- SIPK-overexpression genotypee0
- Treatment
- continuous ozone exposuree0
- Gene
- SIPKe0
- Outcome
- abnormally prolonged activation of SIPKe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During continuous ozone exposure, abnormally prolonged activation of SIPK was seen in the SIPK-overexpression genotype, without WIPK activation, whereas strong and stable activation of WIPK was observed in the SIPK-suppressed lines.”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
WIPK was not activated in the SIPK-overexpression genotype during ozone exposure.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- SIPK-overexpression genotypee0
- Treatment
- continuous ozone exposuree0
- Gene
- WIPKe0
- Outcome
- without WIPK activatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During continuous ozone exposure, abnormally prolonged activation of SIPK was seen in the SIPK-overexpression genotype, without WIPK activation, whereas strong and stable activation of WIPK was observed in the SIPK-suppressed lines.”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
WIPK activation was strong and stable in SIPK-suppressed lines during ozone exposure.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- SIPK-suppressed linese0
- Treatment
- continuous ozone exposuree0
- Gene
- WIPKe0
- Outcome
- strong and stable activation of WIPKe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During continuous ozone exposure, abnormally prolonged activation of SIPK was seen in the SIPK-overexpression genotype, without WIPK activation, whereas strong and stable activation of WIPK was observed in the SIPK-suppressed lines.”
e1“In tobacco, the major ROS-induced MAPK is salicylate-induced protein kinase (SIPK).”
Claim · model-written summary
Activated SIPK may control WIPK inactivation after ROS stimulation in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- SIPKe0
- Outcome
- inactivation of WIPKe0
- Qualifier
- upon ROS stimulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one role of activated SIPK in tobacco cells upon ROS stimulation appears to be control of the inactivation of WIPK”
The same questions in other papers
loss of function · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.