Abstract
We have defined a minimal Arabidopsis CATALASE 3 (CAT3) promoter sufficient to drive evening-specific circadian transcription of a LUCIFERASE reporter gene. Deletion analysis and site-directed mutagenesis reveal a circadian response element, the evening element (EE: AAAATATCT), that is necessary for evening-specific transcription. The EE differs only by a single base pair from the CIRCADIAN CLOCK ASSOCIATED 1-binding site (CBS: AAAAAATCT), which is important for morning-specific transcription. We tested the hypothesis that the EE and the CBS specify circadian phase by site-directed mutagenesis to convert the CAT3 EE into a CBS. Changing the CAT3 EE to a CBS changes the phase of peak transcription from the evening to the morning in continuous dark and in light-dark cycles, consistent with the specification of phase by the single base pair that distinguishes these elements. However, rhythmicity of the CBS-containing CAT3 promoter is dramatically compromised in continuous light. Thus, we conclude that additional information normally provided in the context of a morning-specific promoter is necessary for full circadian activity of the CBS.
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Claims 4
Claim · model-written summary
The evening element (EE) is necessary for evening-specific transcription of the Arabidopsis CAT3 promoter.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- Deletion analysis and site-directed mutagenesise1
- Outcome
- evening-specific transcriptione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion analysis and site-directed mutagenesis reveal a circadian response element, the evening element (EE: AAAATATCT), that is necessary for evening-specific transcription”
t0“Circadian Clock Regulatory Elements in Arabidopsis”
Claim · model-written summary
Changing the CAT3 EE to a CBS shifted peak transcription from the evening to the morning in continuous dark and in light-dark cycles.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Changing the CAT3 EE to a CBSe2
- Outcome
- phase of peak transcriptione2
- Direction
- from the evening to the morninge2
- Comparator
- CAT3 EEe2
- Setting
- continuous dark and in light-dark cyclese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Changing the CAT3 EE to a CBS changes the phase of peak transcription from the evening to the morning in continuous dark and in light-dark cycles”
t0“Circadian Clock Regulatory Elements in Arabidopsis”
Claim · model-written summary
Rhythmicity of the CBS-containing CAT3 promoter is dramatically compromised in continuous light.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- CBS-containing CAT3 promotere3
- Outcome
- rhythmicitye3
- Direction
- dramatically compromisede3
- Setting
- continuous lighte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“rhythmicity of the CBS-containing CAT3 promoter is dramatically compromised in continuous light”
t0“Circadian Clock Regulatory Elements in Arabidopsis”
Claim · model-written summary
Full circadian activity of the CBS requires additional information normally provided by a morning-specific promoter context.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- full circadian activity of the CBSe4
- Qualifier
- additional information normally provided in the context of a morning-specific promotere4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“additional information normally provided in the context of a morning-specific promoter is necessary for full circadian activity of the CBS”
t0“Circadian Clock Regulatory Elements in Arabidopsis”
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