Abstract
The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes.
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Claims 8
Claim · model-written summary
TcZNT5-LC and TcZNT6-LC expression was constitutively high in T. caerulescens relative to Arabidopsis orthologues.
- Taxon
- T. caerulescens
Noccaea caerulescense0 - Gene
- AtZIP5 and AtZIP6e0
- Outcome
- expression of these two genese0
- Direction
- highe0
- Comparator
- AtZIP5 and AtZIP6 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes”
t0“Thlaspi caerulescens”
Claim · model-written summary
The two genes differed in expression between two T. caerulescens accessions.
- Taxon
- T. caerulescensNoccaea caerulescense0
- Material
- two accessions of T. caerulescense0
- Outcome
- differential expression of both genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties”
Claim · model-written summary
TcZNT5-LC expression did not modify Cd or Zn tolerance in A. thaliana.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Treatment
- Expression of TcZNT5-LCe0
- Gene
- TcZNT5-LCe0
- Outcome
- Cd or Zn tolerancee0
- Direction
- did not modifye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance”
Claim · model-written summary
TcZNT5-LC expression mildly shifted root and shoot Zn concentrations toward a hyperaccumulator ratio.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- Expression of TcZNT5-LCe0
- Gene
- TcZNT5-LCe0
- Outcome
- root and shoot Zn concentrationse0
- Qualifier
- towards a hyperaccumulator shoot to root concentration ratioe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio”
Claim · model-written summary
A. thaliana zip5 knock-out mutants had increased Cd tolerance.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- zip5 knock-out mutantse0
- Outcome
- tolerance to Cde0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. thaliana zip5 knock-out mutants showed increased tolerance to Cd”
Claim · model-written summary
A. thaliana zip5 knock-out mutants had decreased seed mineral concentrations.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- zip5 knock-out mutantse0
- Outcome
- seed mineral concentrationse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations”
Claim · model-written summary
TcZNT6-LC expression enhanced Cd sensitivity in A. thaliana.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- Expression of TcZNT6-LCe0
- Gene
- TcZNT6-LCe0
- Outcome
- Cd sensitivitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana”
Claim · model-written summary
The zip6 mutant showed no phenotype.
- Taxon
- Thlaspi caerulescensNoccaea caerulescenst0
- Material
- zip6 mutante0
- Outcome
- no phenotypee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no phenotype was observed for the zip6 mutant”
t0“Thlaspi caerulescens”
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