Abstract
Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L. in liquid N(2). Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in medium containing proline. Cells cryoprotected with proline have an increased recovery potential when compared with cells cryoprotected with dimethylsulfoxide and glycerol. They also show a reduced postthaw viability loss and greater tolerance of a range of postthaw culture conditions. It is suggested that the mechanism of action of proline may be similar to that in its putative role of conferring protection against natural stresses. It may be protecting the cell against solution effects caused by dehydration during freezing. These findings are discussed in relation to other freeze tolerance enhancing treatments.
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Claims 6
Claim · model-written summary
Proline was effective for cryoprotecting cultured Zea mays cells stored in liquid nitrogen.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L. in liquid N(2).”
Claim · model-written summary
Pregrowth for 3 to 4 days in proline medium increased freeze tolerance.
- Treatment
- pregrowth for 3 to 4 days in medium containing prolinee0
- Outcome
- freeze tolerancee0
- Direction
- Increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in medium containing proline.”
t0“Zea mays L”
Claim · model-written summary
Proline cryoprotection increased recovery relative to dimethylsulfoxide and glycerol.
- Treatment
- prolinee0
- Outcome
- recovery potentiale0
- Direction
- increasede0
- Comparator
- dimethylsulfoxide and glycerole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells cryoprotected with proline have an increased recovery potential when compared with cells cryoprotected with dimethylsulfoxide and glycerol.”
t0“Zea mays L”
Claim · model-written summary
Proline cryoprotection reduced postthaw viability loss.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They also show a reduced postthaw viability loss and greater tolerance of a range of postthaw culture conditions.”
t0“Zea mays L”
e1“Cells cryoprotected with proline”
Claim · model-written summary
Proline cryoprotection increased tolerance of postthaw culture conditions.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They also show a reduced postthaw viability loss and greater tolerance of a range of postthaw culture conditions.”
t0“Zea mays L”
e1“Cells cryoprotected with proline”
Claim · model-written summary
The authors suggest proline may protect cells from dehydration-related solution effects during freezing.
- Compound
- Prolinee1
- Outcome
- protecting the cell against solution effectse0
- Qualifier
- dehydration during freezinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It may be protecting the cell against solution effects caused by dehydration during freezing.”
e1“Proline is an effective cryoprotectant”
t0“Zea mays L”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.