Abstract
BACKGROUND AND AIMS: The oomycete Aphanomyces euteiches causes up to 80 % crop loss in pea (Pisum sativum). Aphanomyces euteiches invades the root system leading to a complete arrest of root growth and ultimately to plant death. To date, disease control measures are limited to crop rotation and no resistant pea lines are available. The present study aims to get a deeper understanding of the early oomycete-plant interaction at the tissue and cellular levels. METHODS: Here, the process of root infection by A. euteiches on pea is investigated using flow cytometry and microscopic techniques. Dynamic changes in secondary metabolism are analysed with high-performance liquid chromatography with diode-array detection. KEY RESULTS: Root infection is initiated in the elongation zone but not in the root cap and border cells. Border-cell production is significantly enhanced in response to root inoculation with changes in their size and morphology. The stimulatory effect of A. euteiches on border-cell production is dependent on the number of oospores inoculated. Interestingly, border cells respond to pathogen challenge by increasing the synthesis of the phytoalexin pisatin. CONCLUSIONS: Distinctive responses to A. euteiches inoculation occur at the root tissue level. The findings suggest that root border cells in pea are involved in local defence of the root tip against A. euteiches. Root border cells constitute a convenient quantitative model to measure the molecular and cellular basis of plant-microbe interactions.
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Claims 7
Claim · model-written summary
Pea root infection begins in the elongation zone rather than the root cap or border cells.
- Taxon
- pea
Lathyrus oleraceuse1 - Organ / stage
- elongation zonee0
- Interactor
- A. euteichesAphanomyces euteichese1pathogen
- Outcome
- Root infection is initiated in the elongation zonee0
- Qualifier
- but not in the root cap and border cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root infection is initiated in the elongation zone but not in the root cap and border cells.”
e1“the process of root infection by A. euteiches on pea is investigated”
Claim · model-written summary
Root inoculation significantly enhances pea border-cell production.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- root inoculatione0
- Interactor
- A. euteichesAphanomyces euteichese1pathogen
- Outcome
- Border-cell productione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Border-cell production is significantly enhanced in response to root inoculation with changes in their size and morphology.”
e1“the process of root infection by A. euteiches on pea is investigated”
Claim · model-written summary
Pea root inoculation changes border-cell size and morphology.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- Border-celle0
- Treatment
- root inoculatione0
- Interactor
- A. euteichesAphanomyces euteichese1pathogen
- Outcome
- size and morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Border-cell production is significantly enhanced in response to root inoculation with changes in their size and morphology.”
e1“the process of root infection by A. euteiches on pea is investigated”
Claim · model-written summary
The stimulation of pea border-cell production depends on the number of inoculated oospores.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- number of oospores inoculatede0
- Interactor
- A. euteichesAphanomyces euteichese0pathogen
- Outcome
- border-cell productione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stimulatory effect of A. euteiches on border-cell production is dependent on the number of oospores inoculated.”
e1“the process of root infection by A. euteiches on pea is investigated”
Claim · model-written summary
Pea border cells increase pisatin synthesis after pathogen challenge.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- border cellse0
- Treatment
- pathogen challengee0
- Interactor
- A. euteichesAphanomyces euteichese1pathogen
- Outcome
- synthesis of the phytoalexin pisatine0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, border cells respond to pathogen challenge by increasing the synthesis of the phytoalexin pisatin.”
e1“the process of root infection by A. euteiches on pea is investigated”
Claim · model-written summary
Pea root border cells appear involved in local root-tip defence against A. euteiches.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- root border cellse0
- Interactor
- A. euteichesAphanomyces euteichese0pathogen
- Outcome
- local defence of the root tipe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The findings suggest that root border cells in pea are involved in local defence of the root tip against A. euteiches.”
Claim · model-written summary
Root border cells provide a convenient quantitative model for plant-microbe interactions.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- Root border cellse0
- Outcome
- a convenient quantitative modele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root border cells constitute a convenient quantitative model to measure the molecular and cellular basis of plant-microbe interactions.”
e1“root border cells in pea”
The same questions in other papers
inoculation · Aphanomyces euteiches · Lathyrus oleraceus (pea)Positive and negative results
4 in the opposite direction
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.