Abstract
Abstract A new method to measure enzyme activity of the fungal root pathogen Aphanomyces euteiches in pea roots is described. The specific enzymes of the fungus and the host were separated by polyacrylamide gel electrophoresis (PAGE) and the activity of fungal Glucose‐6‐phosphate dehydrogenase and Phosphoglucomutase were quantified by densitometry. Fungal activity could be correlated to the percentage infected root length and to the disease symptoms of the plants. The activity of A. euteiches was studied in a time course experiment with increasing levels of zoospore inoculum. The results indicated that an increase in inoculum level resulted in a faster disease development in the plants. The relation between fungal enzyme activity and infection level is discussed.
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Claims 4
Claim · model-written summary
PAGE separated fungal and host enzymes, permitting densitometric quantification of fungal enzyme activity.
- Taxon
- Pea Roots
Lathyrus oleraceust0 - Interactor
- Aphanomyces euteichesAphanomyces euteichest0pathogen
- Outcome
- activity of fungal Glucose‐6‐phosphate dehydrogenase and Phosphoglucomutasee0
- Value
- activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specific enzymes of the fungus and the host were separated by polyacrylamide gel electrophoresis (PAGE) and the activity of fungal Glucose‐6‐phosphate dehydrogenase and Phosphoglucomutase were quantified by densitometry”
t0“Aphanomyces euteiches in Pea Roots”
Claim · model-written summary
Fungal activity correlated with percentage infected root length.
- Taxon
- Pea RootsLathyrus oleraceust0
- Organ / stage
- roote0
- Interactor
- Aphanomyces euteichesAphanomyces euteichest0pathogen
- Outcome
- percentage infected root lengthe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fungal activity could be correlated to the percentage infected root length and to the disease symptoms of the plants”
t0“Aphanomyces euteiches in Pea Roots”
Claim · model-written summary
Fungal activity correlated with plant disease symptoms.
- Taxon
- Pea RootsLathyrus oleraceust0
- Organ / stage
- roote0
- Interactor
- Aphanomyces euteichesAphanomyces euteichest0pathogen
- Outcome
- disease symptoms of the plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fungal activity could be correlated to the percentage infected root length and to the disease symptoms of the plants”
t0“Aphanomyces euteiches in Pea Roots”
Claim · model-written summary
Increasing zoospore inoculum caused faster disease development in pea.
- Taxon
- Pea RootsLathyrus oleraceust0
- Treatment
- increase in inoculum levele0
- Interactor
- Aphanomyces euteichesAphanomyces euteichest0pathogen
- Outcome
- disease development in the plantse0
- Direction
- fastere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in inoculum level resulted in a faster disease development in the plants”
t0“Aphanomyces euteiches in Pea Roots”
The same questions in other papers
inoculation · Aphanomyces euteiches · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.