Abstract
XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis. To determine whether XCP1 could be involved in tracheary element autolysis, promoter activity and localization of XCP1 were investigated using XCP1 promoter-beta-glucuronidase fusions and immunofluorescence confocal microscopy. A tracheary element expression pattern was detected for XCP1. Results from confocal microscopy and biochemical subcellular fractionation indicated that XCP1 was localized in the vacuole. Ectopic expression of XCP1 resulted in a reduction in plant size in some lines and early leaf senescence, as indicated by early loss of leaf chlorophyll. Reduced plant size was correlated with higher levels of XCP1, as shown by immunoblot and peptidase activity gel analyses. The XCP1 prodomain exhibits exceptionally high similarity (greater than 80%) to the prodomains of papain and other papain-like enzymes isolated from papaya (Carica papaya) laticifers when compared with all other reported papain-like enzymes. The potential for XCP1 and papain to perform common functions as catalysts of autolytic processing following cell death due to programmed suicide or to wounding is discussed.
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Claims 6
Claim · model-written summary
XCP1 expression was detected in tracheary elements.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- XCP1e0
- Outcome
- tracheary element expression patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A tracheary element expression pattern was detected for XCP1.”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
Claim · model-written summary
XCP1 was localized in the vacuole.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- XCP1e0
- Outcome
- localized in the vacuolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results from confocal microscopy and biochemical subcellular fractionation indicated that XCP1 was localized in the vacuole.”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
Claim · model-written summary
Ectopic XCP1 expression reduced plant size in some lines.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Ectopic expression of XCP1e0
- Gene
- XCP1e0
- Outcome
- plant sizee0
- Direction
- reductione0
- Qualifier
- some linese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of XCP1 resulted in a reduction in plant size in some lines and early leaf senescence”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
Claim · model-written summary
Ectopic XCP1 expression caused early leaf senescence, indicated by early chlorophyll loss.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- leafe0
- Treatment
- Ectopic expression of XCP1e0
- Gene
- XCP1e0
- Outcome
- early leaf senescencee0
- Qualifier
- early loss of leaf chlorophylle0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of XCP1 resulted in a reduction in plant size in some lines and early leaf senescence, as indicated by early loss of leaf chlorophyll.”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
Claim · model-written summary
Smaller plant size correlated with higher XCP1 levels.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- XCP1e0
- Outcome
- plant sizee0
- Direction
- Reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced plant size was correlated with higher levels of XCP1, as shown by immunoblot and peptidase activity gel analyses.”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
Claim · model-written summary
The XCP1 prodomain showed over 80% similarity to papain and other papaya peptidase prodomains relative to other reported enzymes.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- XCP1e0
- Outcome
- similaritye0
- Direction
- exceptionally highe0
- Value
- greater than 80%e0
- Comparator
- papain and other papain-like enzymes isolated from papaya (Carica papaya) laticiferse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The XCP1 prodomain exhibits exceptionally high similarity (greater than 80%) to the prodomains of papain and other papain-like enzymes isolated from papaya (Carica papaya) laticifers when compared with all other reported papain-like enzymes.”
e1“XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis.”
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