Abstract
The molecular mechanisms underlying the initiation and maintenance of the embryonic pathway in plants are largely unknown. To obtain more insight into these processes, we used subtractive hybridization to identify genes that are upregulated during the in vitro induction of embryo development from immature pollen grains of Brassica napus (microspore embryogenesis). One of the genes identified, BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds. Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings. Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology. The expression pattern of BBM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.
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Claims 7
Claim · model-written summary
BABY BOOM resembles AP2/ERF transcription factors.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Gene
- BABY BOOM (BBM)e0
- Outcome
- similarity to the AP2/ERF family of transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors”
e1“immature pollen grains of Brassica napus”
Claim · model-written summary
BBM was preferentially expressed in developing embryos and seeds.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- developing embryos and seedse0
- Gene
- BBMe0
- Outcome
- expressed preferentiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds”
e1“immature pollen grains of Brassica napus”
Claim · model-written summary
Ectopic BBM expression produced somatic embryos and cotyledon-like structures on seedlings.
- Taxon
- Arabidopsis and Brassica
Arabidopsis
Brassicae0 - Treatment
- Ectopic expression of BBMe0
- Gene
- BBMe0
- Outcome
- spontaneous formation of somatic embryos and cotyledon-like structurese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings”
Claim · model-written summary
Ectopic BBM expression induced neoplastic growth.
- Taxon
- Arabidopsis and BrassicaArabidopsisBrassicae1
- Treatment
- Ectopic BBM expressione0
- Gene
- BBMe0
- Outcome
- neoplastic growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology”
e1“Ectopic expression of BBM in Arabidopsis and Brassica”
Claim · model-written summary
Ectopic BBM expression induced hormone-free explant regeneration.
- Taxon
- Arabidopsis and BrassicaArabidopsisBrassicae1
- Treatment
- Ectopic BBM expressione0
- Gene
- BBMe0
- Outcome
- hormone-free regeneration of explantse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology”
e1“Ectopic expression of BBM in Arabidopsis and Brassica”
Claim · model-written summary
Ectopic BBM expression altered leaf and flower morphology.
- Taxon
- Arabidopsis and BrassicaArabidopsisBrassicae1
- Treatment
- Ectopic BBM expressione0
- Gene
- BBMe0
- Outcome
- alterations in leaf and flower morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology”
e1“Ectopic expression of BBM in Arabidopsis and Brassica”
Claim · model-written summary
BBM may promote cell proliferation and morphogenesis during embryogenesis.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BABY BOOMt0
- Outcome
- cell proliferation and morphogenesis during embryogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis”
t0“Ectopic Expression of BABY BOOM”
e1“immature pollen grains of Brassica napus”
The same questions in other papers
overexpression · growth · ArabidopsisPositive and negative results
70 papers in the same direction as this paper · 38 in the opposite direction
- positive
- positive
- positive
105 papers more in this group
See all 109 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.