Abstract
Glycogen synthase kinase 3 (GSK-3) is involved in the regulation of several physiological processes, including glycogen metabolism, protein synthesis, transcription factor activity, and developmental control. Although GSK-3-like genes have been isolated from plants, no function for any of these kinases has been defined. We report here that the alfalfa wound-induced gene (WIG, for wound-induced GSK-3), lencoding a functional plant GSK-3-like kinase, is activated when the alfalfa leaves are wounded. Although WIG transcripts are hardly detectable in mature leaves, WIG mRNA accumulates rapidly after wounding. Using a peptide antibody that specifically recognizes p53(WIG), we show that p53(WIG) kinase is activated immediately after wounding. Wound-induced activation of p53(WIG) kinase is a post-translational process, because the concentrations of p53(WIG) protein do not change in intact and wounded leaves, and inhibition of transcription or translation does not block activation by wounding. However, inactivation of p53(WIG) kinase, which usually occurs within 60 min after wounding, is dependent on transcription and translation of one or more protein factors. These data suggest that the WIG kinase is involved in wound signaling in plants.
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Claims 7
Claim · model-written summary
WIG encodes a functional plant GSK-3-like kinase.
- Taxon
- alfalfa
Medicago sativae0 - Gene
- WIGe0
- Outcome
- functional plant GSK-3-like kinasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the alfalfa wound-induced gene (WIG, for wound-induced GSK-3), lencoding a functional plant GSK-3-like kinase”
Claim · model-written summary
WIG mRNA accumulates rapidly after alfalfa leaf wounding.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- woundinge0
- Gene
- WIGe0
- Outcome
- WIG mRNAe0
- Direction
- accumulates rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WIG mRNA accumulates rapidly after wounding”
e1“alfalfa leaves are wounded”
Claim · model-written summary
p53(WIG) kinase activates immediately after wounding.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- woundinge0
- Gene
- p53(WIG) kinasee0
- Outcome
- p53(WIG) kinasee0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“p53(WIG) kinase is activated immediately after wounding”
e1“alfalfa leaves are wounded”
Claim · model-written summary
p53(WIG) protein concentrations do not change between intact and wounded leaves.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- wounded leavese0
- Gene
- p53(WIG) proteine0
- Outcome
- concentrations of p53(WIG) proteine0
- Direction
- do not changee0
- Comparator
- intacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“concentrations of p53(WIG) protein do not change in intact and wounded leaves”
e1“alfalfa leaves are wounded”
Claim · model-written summary
Inhibition of transcription or translation does not block wound activation of p53(WIG).
- Taxon
- alfalfaMedicago sativae1
- Treatment
- inhibition of transcription or translatione0
- Outcome
- activation by woundinge0
- Direction
- does not blocke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of transcription or translation does not block activation by wounding”
e1“alfalfa leaves are wounded”
Claim · model-written summary
p53(WIG) kinase inactivation usually occurs within 60 minutes of wounding and depends on transcription and translation.
- Taxon
- alfalfaMedicago sativae1
- Gene
- p53(WIG) kinasee0
- Outcome
- inactivation of p53(WIG) kinasee0
- Value
- 60 mine0
- Qualifier
- transcription and translation of one or more protein factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inactivation of p53(WIG) kinase, which usually occurs within 60 min after wounding, is dependent on transcription and translation of one or more protein factors”
e1“alfalfa leaves are wounded”
Claim · model-written summary
The authors infer that WIG kinase participates in plant wound signaling.
- Taxon
- alfalfaMedicago sativae1
- Gene
- WIG kinasee0
- Outcome
- wound signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the WIG kinase is involved in wound signaling in plants”
e1“alfalfa leaves are wounded”
The same questions in other papers
mechanical · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- no effect
- no effect
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.