Abstract
Previous studies established that excised leaf tissues of alfalfa can be inoculated with Sclerotinia trifoliorum to select for heritable resistance, but the original procedures were not practical for use in large-scale screening programs. In this study, simplified leaf inoculation procedures for more rapid screening for resistance, based on direct application of leaf tissues to colonies of the pathogen on agar media, were evaluated. Cotyledons, unifoliate leaves, and leaflets of trifoliolate leaves of plants 7 to 21 days old from three relatively susceptible cultivars and one resistant germ plasm were applied, with and without wounding, directly to colony margins of S. trifoliorum on cornmeal agar, V8 juice agar, and water agar. Leaves were scored according to the rate and extent of development of necrosis. Significant differences between alfalfa populations were expressed in unifoliate leaves and trifoliolate leaflets but not in cotyledons. Disease severity in the resistant germ plasm (Mississippi Sclerotinia-Resistant [MSR]) was less than in the three cultivars on all agar media. Wounding of leaf tissues increased disease severity and greatly reduced the incidence of symptomless leaves, which are indicated to be escapes, but wounding generally did not prevent expression of resistance in MSR. Results indicate that initial screening for resistance to S. trifoliorum in alfalfa seedlings may be accomplished by applying wounded unifoliate leaves and leaflets of trifoliolate leaves directly to colonies on cornmeal or V8 juice agars.
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Claims 7
Claim · model-written summary
Significant differences between alfalfa populations were expressed in unifoliate leaves and trifoliolate leaflets
- Taxon
- Alfalfa
Medicago sativat0 - Organ / stage
- unifoliate leaves and trifoliolate leafletse1
- Outcome
- differences between alfalfa populationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e1“Significant differences between alfalfa populations were expressed in unifoliate leaves and trifoliolate leaflets but not in cotyledons”
Claim · model-written summary
No significant differences between alfalfa populations were expressed in cotyledons
- Taxon
- AlfalfaMedicago sativat0
- Organ / stage
- cotyledonse1
- Outcome
- differences between alfalfa populationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e1“Significant differences between alfalfa populations were expressed in unifoliate leaves and trifoliolate leaflets but not in cotyledons”
Claim · model-written summary
Disease severity in the Mississippi Sclerotinia-Resistant germplasm was less than in the three cultivars on all agar media
- Taxon
- AlfalfaMedicago sativat0
- Material
- Mississippi Sclerotinia-Resistante3
- Outcome
- Disease severitye3
- Direction
- lesse3
- Comparator
- three cultivarse3
- Setting
- all agar mediae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e3“Disease severity in the resistant germ plasm (Mississippi Sclerotinia-Resistant [MSR]) was less than in the three cultivars on all agar media”
Claim · model-written summary
Wounding of leaf tissues increased disease severity in alfalfa leaves
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Wounding of leaf tissuese5
- Outcome
- disease severitye5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e5“Wounding of leaf tissues increased disease severity and greatly reduced the incidence of symptomless leaves”
Claim · model-written summary
Wounding of leaf tissues greatly reduced the incidence of symptomless leaves
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Wounding of leaf tissuese5
- Outcome
- incidence of symptomless leavese5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e5“Wounding of leaf tissues increased disease severity and greatly reduced the incidence of symptomless leaves”
Claim · model-written summary
Wounding did not prevent expression of resistance in the MSR germplasm
- Taxon
- AlfalfaMedicago sativat0
- Material
- MSRe6
- Treatment
- woundinge6
- Outcome
- expression of resistancee6
- Direction
- no_changeinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e6“but wounding generally did not prevent expression of resistance in MSR”
Claim · model-written summary
Initial screening for resistance may be done by applying wounded unifoliate leaves and leaflets directly to pathogen colonies on cornmeal or V8 juice agars
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- initial screening for resistancee7
- Setting
- cornmeal or V8 juice agarse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Alfalfa Seedlings”
e7“initial screening for resistance to S. trifoliorum in alfalfa seedlings may be accomplished by applying wounded unifoliate leaves and leaflets of trifoliolate leaves directly to colonies on cornmeal or V8 juice agars”
The same questions in other papers
mechanical · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.