Abstract
Real-time quantitative PCR was used to study lipoxygenase (LOX) gene expression patterns in kiwifruit (Actinidia deliciosa [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward) during fruit ripening, and in response to ethylene and low temperature during post-harvest storage. Six LOX genes were identified and cloned from a kiwifruit EST database. All were expressed in vegetative tissues and in the fruit. Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage and were up-regulated by ethylene treatment, following a similar pattern to LOX enzyme activity. By contrast, AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation, and ethylene application enhanced the decline in transcript levels. Transcripts of AdLox6 declined with fruit ripening. The fruit showed no ripening changes at low temperature, where transcripts of AdLox1 and AdLox6 were slightly induced about 72 h after harvest, suggesting an adaptive response to low temperature. Transient expression of the ethylene-responsive AdLox1 gene in tobacco leaves led to significant degradation of chlorophyll and promoted tissue senescence, whereas AdLox2 had no such effect. The results showed that the six LOX genes were differentially regulated during kiwifruit ripening and senescence, forming two groups, one active in ripening and responsive to ethylene and the other more constitutively expressed. The possible roles of individual LOX isoforms in kiwifruit are discussed.
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Claims 19
Claim · model-written summary
Six LOX genes were identified and cloned from a kiwifruit EST database.
- Taxon
- kiwifruit
Actinidia deliciosae0 - Outcome
- LOX genese0
- Value
- Sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six LOX genes were identified and cloned from a kiwifruit EST database.”
Claim · model-written summary
All six LOX genes were expressed in vegetative tissues and fruit.
- Taxon
- kiwifruitActinidia deliciosat0
- Organ / stage
- vegetative tissues and in the fruite0
- Outcome
- expressed in vegetative tissues and in the fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All were expressed in vegetative tissues and in the fruit.”
t0“kiwifruit”
Claim · model-written summary
AdLox1 expression rose toward the fruit climacteric stage.
- Taxon
- kiwifruitActinidia deliciosat0
- Organ / stage
- fruite0
- Gene
- AdLox1e0
- Outcome
- Expression of AdLox1e0
- Direction
- increased markedlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage”
t0“kiwifruit”
Claim · model-written summary
AdLox5 expression rose toward the fruit climacteric stage.
- Taxon
- kiwifruitActinidia deliciosat0
- Organ / stage
- fruite0
- Gene
- AdLox5e0
- Outcome
- AdLox5e0
- Direction
- increased markedlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage”
t0“kiwifruit”
Claim · model-written summary
Ethylene treatment up-regulated AdLox1.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- ethylene treatmente0
- Gene
- AdLox1e1
- Outcome
- AdLox1e1
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were up-regulated by ethylene treatment, following a similar pattern to LOX enzyme activity.”
e1“Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage”
t0“kiwifruit”
Claim · model-written summary
Ethylene treatment up-regulated AdLox5.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- ethylene treatmente0
- Gene
- AdLox5e1
- Outcome
- AdLox5e1
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were up-regulated by ethylene treatment, following a similar pattern to LOX enzyme activity.”
e1“Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage”
t0“kiwifruit”
Claim · model-written summary
AdLox2 transcripts were negatively associated with ethylene accumulation.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AdLox2e0
- Compound
- ethylenee0
- Outcome
- AdLox2e0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
AdLox3 transcripts were negatively associated with ethylene accumulation.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AdLox3e0
- Compound
- ethylenee0
- Outcome
- AdLox3e0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
AdLox4 transcripts were negatively associated with ethylene accumulation.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AdLox4e0
- Compound
- ethylenee0
- Outcome
- AdLox4e0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
Ethylene application accelerated decline of AdLox2 transcripts.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- ethylene applicatione0
- Gene
- AdLox2e1
- Outcome
- transcript levelse0
- Direction
- enhanced the declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene application enhanced the decline in transcript levels.”
e1“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
Ethylene application accelerated decline of AdLox3 transcripts.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- ethylene applicatione0
- Gene
- AdLox3e1
- Outcome
- transcript levelse0
- Direction
- enhanced the declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene application enhanced the decline in transcript levels.”
e1“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
Ethylene application accelerated decline of AdLox4 transcripts.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- ethylene applicatione0
- Gene
- AdLox4e1
- Outcome
- transcript levelse0
- Direction
- enhanced the declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene application enhanced the decline in transcript levels.”
e1“AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation”
t0“kiwifruit”
Claim · model-written summary
AdLox6 transcripts declined during fruit ripening.
- Taxon
- kiwifruitActinidia deliciosat0
- Organ / stage
- fruit ripeninge0
- Gene
- AdLox6e0
- Outcome
- Transcripts of AdLox6e0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcripts of AdLox6 declined with fruit ripening.”
t0“kiwifruit”
Claim · model-written summary
Kiwifruit showed no ripening changes at low temperature.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- low temperaturee0
- Outcome
- ripening changese0
- Direction
- no ripening changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fruit showed no ripening changes at low temperature”
t0“kiwifruit”
Claim · model-written summary
AdLox1 transcripts were slightly induced at low temperature about 72 h after harvest.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- low temperaturee1
- Outcome
- transcripts of AdLox1e0
- Direction
- slightly inducede0
- Value
- 72 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts of AdLox1 and AdLox6 were slightly induced about 72 h after harvest”
e1“The fruit showed no ripening changes at low temperature”
t0“kiwifruit”
Claim · model-written summary
AdLox6 transcripts were slightly induced at low temperature about 72 h after harvest.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- low temperaturee1
- Outcome
- AdLox6e0
- Direction
- slightly inducede0
- Value
- 72 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts of AdLox1 and AdLox6 were slightly induced about 72 h after harvest”
e1“The fruit showed no ripening changes at low temperature”
t0“kiwifruit”
Claim · model-written summary
Transient AdLox1 expression caused chlorophyll degradation in tobacco leaves.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Transient expression of the ethylene-responsive AdLox1 genee0
- Gene
- AdLox1e0
- Outcome
- degradation of chlorophylle0
- Direction
- degradatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of the ethylene-responsive AdLox1 gene in tobacco leaves led to significant degradation of chlorophyll and promoted tissue senescence”
Claim · model-written summary
Transient AdLox1 expression promoted senescence in tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Transient expression of the ethylene-responsive AdLox1 genee0
- Gene
- AdLox1e0
- Outcome
- tissue senescencee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of the ethylene-responsive AdLox1 gene in tobacco leaves led to significant degradation of chlorophyll and promoted tissue senescence”
Claim · model-written summary
AdLox2 expression did not produce the chlorophyll or senescence effects.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- AdLox2e0
- Outcome
- no such effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas AdLox2 had no such effect.”
t0“tobacco leaves”
The same questions in other papers
transgenic · quality: postharvest · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
cold · quality: postharvest · Actinidia deliciosa (kiwifruit)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →transgenic · physiology: pigments · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.