Abstract
A full_length cDNA has been cloned encoding nicotinamide adenine dinucleotide phosphate_specific glutamate dehydrogenase (NADP_GDH) from Chlorella sorokiniana with the RT_PCR method. The complete nucleotide sequence of NADP_GDH gene had 94% homology to the previously reported one . The NADP_GDH gene was constructed into a vector highly expressed in plants. The specific activity of NADP_GDH in transformants was detected, but not in the control plants. All transformed shoots on MS medium containing lower concentration of nitrogen and the transformed seedlings grown in lower concentration of nitrogen vermiculite had higher growth rate and more leaves than the control plants. Transformed leaf discs cultured on MS medium containing different nitrogen concentrations had more chlorophyll contents compared to the controls. These results suggested that exogenous NADP_GDH may enhance the absorption and utilization to ammonium in plants. The increased weight of transformed leaf discs cultured on medium supplemented with different concentrations of phosphinothricin (PPT) was more than that of control discs. 0.5 μg/mL PPT could be used as a selecting drug instead of kanamycin to develop the transformants. These results suggested that the NADP_GDH gene might be used as a new selecting gene in the future research of plant gene engineering.
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Claims 8
Claim · model-written summary
The cloned NADP_GDH sequence had 94% homology with a previously reported sequence.
- Taxon
- Tobacco Plants
Nicotiana tabacumt0 - Gene
- NADP_GDHe0
- Outcome
- homologye0
- Value
- 94%e0
- Comparator
- the previously reported onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The complete nucleotide sequence of NADP_GDH gene had 94% homology to the previously reported one”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
NADP_GDH activity was detected in transformants but not controls.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- transformantse0
- Gene
- NADP_GDHe0
- Outcome
- specific activity of NADP_GDHe0
- Comparator
- control plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specific activity of NADP_GDH in transformants was detected, but not in the control plants”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Under low nitrogen, transformed shoots grew faster and had more leaves than controls.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- transformed shootse0
- Outcome
- growth rate and more leavese0
- Direction
- highere0
- Qualifier
- MS medium containing lower concentration of nitrogene0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All transformed shoots on MS medium containing lower concentration of nitrogen and the transformed seedlings grown in lower concentration of nitrogen vermiculite had higher growth rate and more leaves than the control plants”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Under low nitrogen, transformed seedlings grew faster and had more leaves than controls.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- transformed seedlingse0
- Outcome
- growth rate and more leavese0
- Direction
- highere0
- Qualifier
- lower concentration of nitrogen vermiculitee0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transformed seedlings grown in lower concentration of nitrogen vermiculite had higher growth rate and more leaves than the control plants”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Transformed leaf discs had more chlorophyll than controls across nitrogen concentrations.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- Transformed leaf discse0
- Outcome
- chlorophyll contentse0
- Direction
- moree0
- Qualifier
- different nitrogen concentrationse0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformed leaf discs cultured on MS medium containing different nitrogen concentrations had more chlorophyll contents compared to the controls”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Transformed leaf discs gained more weight than controls with phosphinothricin.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- transformed leaf discse0
- Outcome
- weighte0
- Direction
- moree0
- Qualifier
- different concentrations of phosphinothricin (PPT)e0
- Comparator
- control discse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increased weight of transformed leaf discs cultured on medium supplemented with different concentrations of phosphinothricin (PPT) was more than that of control discs”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
The authors proposed 0.5 μg/mL PPT for transformant selection instead of kanamycin.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Treatment
- 0.5 μg/mL PPTe0
- Outcome
- selecting druge0
- Comparator
- kanamycine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.5 μg/mL PPT could be used as a selecting drug instead of kanamycin to develop the transformants”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
The authors proposed NADP_GDH as a future selectable gene.
- Taxon
- Tobacco PlantsNicotiana tabacumt0
- Gene
- NADP_GDHe0
- Outcome
- new selecting genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NADP_GDH gene might be used as a new selecting gene in the future research of plant gene engineering”
t0“Transgenic Tobacco Plants”
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.