Abstract
Two potato varieties namely, Almera and Diamant were induced to form micro tubers under two in vitro culture conditions (darkness and light). Murashige and Skoog (MS) medium verified with varied concentrations of thiadizuron (TDZ), benzylaminopurine BAP and Sucrose, were evaluated for micro tuber induction using node segment explant from the in vitro culture micro plant. Highest micro tubers number (6.0±0.5 micro tuber/jar) obtained by Almera on MS medium verified with sucrose 8% only under dark, whereas higher micro tuber number obtained by Diamant cultivar is (3.0±0.0 micro tuber/jar) on MS medium verified with sucrose 8% only at dark too. In case of micro tuber weight, maximum tuber weight (1250.3±13.0 mg/tuber) obtained by Almera on MS medium supported with 60 g/l sucrose without hormone in dark. Higher micro tuber weight obtained by Diamant cultivar was (420.9±1.3) mg/tuber obtained on MS medium supported with 60 g/l sucrose in dark, followed by (248.6±25.5) mg/tuber produced on MS medium verified with 60.0 g/l sucrose plus 5.0 mg/l TDZ under light, at 30g/l sucrose no micro tuber observed. Highest micro tuber number is related to high sucrose concentration than the level of growth hormones in the medium. It was also observed that twenty four hour dark was best for tuber initiation.
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Claims 8
Claim · model-written summary
On MS medium with 8% sucrose in the dark, Almera gave the highest micro tuber number, 6.0±0.5 per jar.
- Taxon
- Solanum tuberosum L.
Solanum tuberosumt0 - Material
- Almerae0
- Treatment
- MS medium verified with sucrose 8% onlye0
- Outcome
- micro tubers numbere0
- Direction
- Higheste0
- Value
- 6.0±0.5 micro tuber/jare0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e0“Highest micro tubers number (6.0±0.5 micro tuber/jar) obtained by Almera on MS medium verified with sucrose 8% only under dark”
Claim · model-written summary
On the same medium in the dark, Diamant gave 3.0±0.0 micro tubers per jar, a higher micro tuber number than Almera was reported for it.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Material
- Diamante1
- Treatment
- MS medium verified with sucrose 8% onlye1
- Outcome
- micro tuber numbere1
- Direction
- highere1
- Value
- 3.0±0.0 micro tuber/jare1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e1“whereas higher micro tuber number obtained by Diamant cultivar is (3.0±0.0 micro tuber/jar) on MS medium verified with sucrose 8% only at dark too”
Claim · model-written summary
Almera gave the maximum tuber weight, 1250.3±13.0 mg per tuber, on MS medium with 60 g/l sucrose and no hormone in the dark.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Material
- Almerae4
- Treatment
- MS medium supported with 60 g/l sucrose without hormonee4
- Outcome
- tuber weighte4
- Direction
- maximume4
- Value
- 1250.3±13.0 mg/tubere4
- Qualifier
- in darke4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e4“maximum tuber weight (1250.3±13.0 mg/tuber) obtained by Almera on MS medium supported with 60 g/l sucrose without hormone in dark”
Claim · model-written summary
Diamant gave a higher micro tuber weight, (420.9±1.3) mg per tuber, on MS medium with 60 g/l sucrose in the dark.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Material
- Diamant cultivare3
- Treatment
- MS medium supported with 60 g/l sucrose in darke3
- Outcome
- micro tuber weighte3
- Direction
- Highere3
- Value
- (420.9±1.3) mg/tubere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e3“Higher micro tuber weight obtained by Diamant cultivar was (420.9±1.3) mg/tuber obtained on MS medium supported with 60 g/l sucrose in dark, followed by (248.6±25.5) mg/tuber produced on MS medium verified with 60.0 g/l sucrose plus 5.0 mg/l TDZ under light”
Claim · model-written summary
Diamant micro tuber weight on MS medium with 60 g/l sucrose plus 5.0 mg/l TDZ under light was lower than in the dark, at (248.6±25.5) mg per tuber.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Material
- Diamant cultivare3
- Treatment
- MS medium verified with 60.0 g/l sucrose plus 5.0 mg/l TDZ under lighte3
- Outcome
- micro tuber weighte3
- Direction
- lowerinferrede3
- Value
- (248.6±25.5) mg/tubere3
- Comparator
- MS medium supported with 60 g/l sucrose in darke3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e3“Higher micro tuber weight obtained by Diamant cultivar was (420.9±1.3) mg/tuber obtained on MS medium supported with 60 g/l sucrose in dark, followed by (248.6±25.5) mg/tuber produced on MS medium verified with 60.0 g/l sucrose plus 5.0 mg/l TDZ under light”
Claim · model-written summary
At 30 g/l sucrose no micro tubers were observed.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Treatment
- 30g/l sucrosee5
- Outcome
- micro tuber observede5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e5“at 30g/l sucrose no micro tuber observed”
Claim · model-written summary
The highest micro tuber number is related to a high sucrose concentration rather than to the level of growth hormones in the medium.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Treatment
- high sucrose concentratione2
- Outcome
- micro tuber numbere2
- Comparator
- the level of growth hormonese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e2“Highest micro tuber number is related to high sucrose concentration than the level of growth hormones in the medium”
Claim · model-written summary
Twenty-four hour darkness was best for potato tuber initiation in vitro.
- Taxon
- Solanum tuberosum L.Solanum tuberosumt0
- Treatment
- twenty four hour darke6
- Outcome
- tuber initiatione6
- Direction
- beste6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum L.)”
e6“It was also observed that twenty four hour dark was best for tuber initiation”
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