Abstract
The vsp1 gene of Arabidopsis thaliana encodes a storage protein that accumulates in vegetative organs. Transgenic plants expressing a vsp1 promoter-gus (beta-glucuronidase) gene fusion were found to contain high GUS activity when challenged with jasmonate, a volatile plant hormone. The induction of vsp1-gus expression by jasmonate could be measured in tobacco mesophyll protoplasts, after transient expression. A number of deletions were operated in the vsp1 promoter in order to locate its jasmonate-responsive element. A 41 bp sequence taken approximately 150 bp upstream of the vsp1 TATA box could confer jasmonate responsiveness upon a short CaMV 35S promoter. Whereas the deletion of a CAAT box-like element within the 41 bp sequence did not affect promoter activity, mutation of a short palindromic motif completely abolished jasmonate responsiveness. This motif shows no homology with the jasmonate-responsive elements of other promoters.
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Claims 6
Claim · model-written summary
Jasmonate challenge induced high GUS activity in vsp1 promoter-gus plants.
- Taxon
- A. thaliana
Arabidopsis thalianat0 - Treatment
- jasmonatee0
- Gene
- vsp1 promoter-gus (beta-glucuronidase)e0
- Outcome
- GUS activitye0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants expressing a vsp1 promoter-gus (beta-glucuronidase) gene fusion were found to contain high GUS activity when challenged with jasmonate”
t0“A. thaliana”
Claim · model-written summary
Jasmonate induced vsp1-gus expression in tobacco protoplasts.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- mesophyll protoplastse0
- Treatment
- jasmonatee0
- Gene
- vsp1-guse0
- Outcome
- vsp1-gus expressione0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of vsp1-gus expression by jasmonate could be measured in tobacco mesophyll protoplasts, after transient expression.”
Claim · model-written summary
A 41 bp vsp1 promoter sequence conferred jasmonate responsiveness on a short CaMV 35S promoter.
- Taxon
- A. thalianaArabidopsis thalianat0
- Treatment
- A 41 bp sequencee0
- Gene
- vsp1 TATA boxe0
- Outcome
- jasmonate responsivenesse0
- Qualifier
- approximately 150 bp upstreame0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 41 bp sequence taken approximately 150 bp upstream of the vsp1 TATA box could confer jasmonate responsiveness upon a short CaMV 35S promoter.”
t0“A. thaliana”
Claim · model-written summary
Deleting the CAAT box-like element did not affect promoter activity.
- Taxon
- A. thalianaArabidopsis thalianat0
- Treatment
- deletion of a CAAT box-like elemente0
- Outcome
- promoter activitye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion of a CAAT box-like element within the 41 bp sequence did not affect promoter activity”
t0“A. thaliana”
Claim · model-written summary
Mutating the palindromic motif abolished jasmonate responsiveness.
- Taxon
- A. thalianaArabidopsis thalianat0
- Treatment
- mutation of a short palindromic motife0
- Outcome
- jasmonate responsivenesse0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of a short palindromic motif completely abolished jasmonate responsiveness”
t0“A. thaliana”
Claim · model-written summary
The motif had no homology with other jasmonate-responsive elements.
- Taxon
- A. thalianaArabidopsis thalianat0
- Outcome
- no homologye0
- Comparator
- jasmonate-responsive elements of other promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This motif shows no homology with the jasmonate-responsive elements of other promoters.”
t0“A. thaliana”
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