Abstract
At least 12 avirulence genes have been genetically identified and mapped in Phytophthora sojae, an oomycete pathogen causing root and stem rot of soybean. Previously, the Avr4 and Avr6 genes of P. sojae were genetically mapped within a 24 kb interval of the genome. Here, we identify Avr4 and Avr6 and show that they are actually a single gene, Avr4/6, located near the 24-kb region. Avr4/6 encodes a secreted protein of 123 amino acids with an RXLR-dEER protein translocation motif. Transient expression of Avr4/6 in soybean leaves revealed that its gene product could trigger a hypersensitive response (HR) in the presence of either Rps4 or Rps6. Silencing Avr4/6 in P. sojae stable transformants abolished the avirulence phenotype exhibited on both Rps4 and Rps6 soybean cultivars. The N terminus of Avr4/6, including the dEER motif, is sufficient to trigger Rps4-dependent HR while its C terminus is sufficient to trigger Rps6-mediated HR. Compared with alleles from avirulent races, alleles of Avr4/6 from virulent races possess nucleotide substitutions in the 5' untranslated region of the gene but not in the protein-coding region.
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Claims 9
Claim · model-written summary
Avr4 and Avr6 are one gene, Avr4/6, near the mapped 24-kb region.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- Phytophthora sojaePhytophthora sojaet1pathogen
- Gene
- Avr4/6e0
- Outcome
- a single gene, Avr4/6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we identify Avr4 and Avr6 and show that they are actually a single gene, Avr4/6, located near the 24-kb region”
t0“Soybean”
t1“Phytophthora sojae”
Claim · model-written summary
Avr4/6 encodes a 123-amino-acid secreted protein with an RXLR-dEER motif.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Phytophthora sojaePhytophthora sojaet1pathogen
- Gene
- Avr4/6e0
- Outcome
- secreted proteine0
- Value
- 123 amino acidse0
- Qualifier
- RXLR-dEER protein translocation motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avr4/6 encodes a secreted protein of 123 amino acids with an RXLR-dEER protein translocation motif”
t0“Soybean”
t1“Phytophthora sojae”
Claim · model-written summary
Transient Avr4/6 expression triggered hypersensitive response with Rps4 in soybean leaves.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Transient expression of Avr4/6e0
- Interactor
- Phytophthora sojaePhytophthora sojaet0pathogen
- Gene
- Rps4e0
- Outcome
- hypersensitive response (HR)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of Avr4/6 in soybean leaves revealed that its gene product could trigger a hypersensitive response (HR) in the presence of either Rps4 or Rps6”
t0“Phytophthora sojae”
Claim · model-written summary
Transient Avr4/6 expression triggered hypersensitive response with Rps6 in soybean leaves.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Transient expression of Avr4/6e0
- Interactor
- Phytophthora sojaePhytophthora sojaet0pathogen
- Gene
- Rps6e0
- Outcome
- hypersensitive response (HR)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of Avr4/6 in soybean leaves revealed that its gene product could trigger a hypersensitive response (HR) in the presence of either Rps4 or Rps6”
t0“Phytophthora sojae”
Claim · model-written summary
Silencing Avr4/6 abolished avirulence on Rps4 soybean cultivars.
- Taxon
- soybeanGlycine maxe0
- Material
- Rps4e0
- Treatment
- Silencing Avr4/6e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- Avr4/6e0
- Outcome
- avirulence phenotypee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing Avr4/6 in P. sojae stable transformants abolished the avirulence phenotype exhibited on both Rps4 and Rps6 soybean cultivars”
Claim · model-written summary
Silencing Avr4/6 abolished avirulence on Rps6 soybean cultivars.
- Taxon
- soybeanGlycine maxe0
- Material
- Rps6e0
- Treatment
- Silencing Avr4/6e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- Avr4/6e0
- Outcome
- avirulence phenotypee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing Avr4/6 in P. sojae stable transformants abolished the avirulence phenotype exhibited on both Rps4 and Rps6 soybean cultivars”
Claim · model-written summary
The Avr4/6 N terminus including dEER is sufficient for Rps4-dependent hypersensitive response.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Phytophthora sojaePhytophthora sojaet1pathogen
- Gene
- Avr4/6e0
- Outcome
- Rps4-dependent HRe0
- Qualifier
- N terminus of Avr4/6, including the dEER motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The N terminus of Avr4/6, including the dEER motif, is sufficient to trigger Rps4-dependent HR”
t0“Soybean”
t1“Phytophthora sojae”
Claim · model-written summary
The Avr4/6 C terminus is sufficient for Rps6-mediated hypersensitive response.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Phytophthora sojaePhytophthora sojaet1pathogen
- Gene
- Avr4/6e1
- Outcome
- Rps6-mediated HRe0
- Qualifier
- C terminuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its C terminus is sufficient to trigger Rps6-mediated HR”
e1“The N terminus of Avr4/6”
t0“Soybean”
t1“Phytophthora sojae”
Claim · model-written summary
Virulent Avr4/6 alleles have 5-prime untranslated-region substitutions relative to avirulent alleles, but none in the coding region.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Phytophthora sojaePhytophthora sojaet1pathogen
- Gene
- Avr4/6e0
- Outcome
- nucleotide substitutions in the 5' untranslated region of the gene but not in the protein-coding regione0
- Comparator
- alleles from avirulent racese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with alleles from avirulent races, alleles of Avr4/6 from virulent races possess nucleotide substitutions in the 5' untranslated region of the gene but not in the protein-coding region”
t0“Soybean”
t1“Phytophthora sojae”
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.