Abstract
The perception of Phytophthora sojae avirulence (Avr) gene products by corresponding soybean resistance (Rps) gene products causes effector triggered immunity. Past studies have shown that the Avr3a and Avr5 genes of P. sojae are genetically linked, and the Avr3a gene encoding a secreted RXLR effector protein was recently identified. We now provide evidence that Avr3a and Avr5 are allelic. Genetic mapping data from F(2) progeny indicates that Avr3a and Avr5 co-segregate, and haplotype analysis of P. sojae strain collections reveal sequence and transcriptional polymorphisms that are consistent with a single genetic locus encoding Avr3a/5. Transformation of P. sojae and transient expression in soybean were performed to test how Avr3a/5 alleles interact with soybean Rps3a and Rps5. Over-expression of Avr3a/5 in a P. sojae strain that is normally virulent on Rps3a and Rps5 results in avirulence to Rps3a and Rps5; whereas silencing of Avr3a/5 causes gain of virulence in a P. sojae strain that is normally avirulent on Rps3a and Rps5 soybean lines. Transient expression and co-bombardment with a reporter gene confirms that Avr3a/5 triggers cell death in Rps5 soybean leaves in an appropriate allele-specific manner. Sequence analysis of the Avr3a/5 gene identifies crucial residues in the effector domain that distinguish recognition by Rps3a and Rps5.
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Claims 6
Claim · model-written summary
Avr3a and Avr5 alleles co-segregated.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- Phytophthora sojaePhytophthora sojaet0pathogen
- Gene
- Avr3a and Avr5e0
- Outcome
- co-segregatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic mapping data from F(2) progeny indicates that Avr3a and Avr5 co-segregate”
t0“Sequence Variants of the Phytophthora sojae RXLR Effector Avr3a/5 Are Differentially Recognized by Rps3a and Rps5 in Soybean”
Claim · model-written summary
Phytophthora sojae haplotypes and transcription patterns supported a single Avr3a/5 locus.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- Avr3a/5e0
- Outcome
- single genetic locus encoding Avr3a/5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haplotype analysis of P. sojae strain collections reveal sequence and transcriptional polymorphisms that are consistent with a single genetic locus encoding Avr3a/5”
t0“Soybean”
Claim · model-written summary
Avr3a/5 overexpression made a normally virulent strain avirulent on Rps3a and Rps5 soybean.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Over-expression of Avr3a/5e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- Avr3a/5e0
- Outcome
- avirulence to Rps3a and Rps5e0
- Comparator
- normally virulente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of Avr3a/5 in a P. sojae strain that is normally virulent on Rps3a and Rps5 results in avirulence to Rps3a and Rps5”
t0“Soybean”
Claim · model-written summary
Avr3a/5 silencing caused gain of virulence on Rps3a and Rps5 soybean lines.
- Taxon
- soybeanGlycine maxe0
- Treatment
- silencing of Avr3a/5e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- Avr3a/5e0
- Outcome
- virulencee0
- Direction
- gaine0
- Comparator
- normally avirulente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of Avr3a/5 causes gain of virulence in a P. sojae strain that is normally avirulent on Rps3a and Rps5 soybean lines”
Claim · model-written summary
Avr3a/5 triggered allele-specific cell death in Rps5 soybean leaves.
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Avr3a/5 triggers cell death in Rps5 soybean leaves in an appropriate allele-specific manner”
Claim · model-written summary
Effector-domain residues distinguished Rps3a and Rps5 recognition.
- Taxon
- SoybeanGlycine maxt0
- Gene
- Avr3a/5e0
- Outcome
- recognition by Rps3a and Rps5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analysis of the Avr3a/5 gene identifies crucial residues in the effector domain that distinguish recognition by Rps3a and Rps5”
t0“Soybean”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
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- no effect
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