Abstract
The coding regions from five avirulence (avr) genes from Xanthomonas campestris pv. malvacearum (Xcm), the causal agent of bacterial blight of cotton, were joined to the cauliflower mosaic virus 35S promoter and expressed transiently in cotton leaves after Agrobacterium tumefaciens-mediated gene transfer. A genotype-specific necrosis was observed 3 to 6 days post inoculation for constructs derived from avrB4, avrb7, and avrBIn on cotton lines containing the resistance (R) genes B4, b7, and BIn, respectively, but not on susceptible cotton plants. No necrosis was obtained with plasmids that direct expression of avr genes within A. tumefaciens, showing that the cotton response required transfer of the genes into the plant cells. Addition of a signal peptide sequence into the avr constructs to target expressed Avr protein to the apoplast significantly reduced the responses. The results indicate that intracellular expression of Xcm Avr proteins in cotton having the corresponding R gene causes specific host cell death.
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Claims 6
Claim · model-written summary
avrB4 elicited necrosis on cotton lines with B4 but not susceptible cotton.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- constructs derived from avrB4e0
- Gene
- avrB4e0
- Outcome
- necrosise0
- Direction
- observede0
- Value
- 3 to 6 dayse0
- Comparator
- susceptible cotton plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A genotype-specific necrosis was observed 3 to 6 days post inoculation for constructs derived from avrB4, avrb7, and avrBIn on cotton lines containing the resistance (R) genes B4, b7, and BIn, respectively, but not on susceptible cotton plants.”
Claim · model-written summary
avrb7 elicited necrosis on cotton lines with b7 but not susceptible cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- constructs derived from avrB4, avrb7, and avrBIne0
- Gene
- avrb7e0
- Outcome
- necrosise0
- Direction
- observede0
- Comparator
- susceptible cotton plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A genotype-specific necrosis was observed 3 to 6 days post inoculation for constructs derived from avrB4, avrb7, and avrBIn on cotton lines containing the resistance (R) genes B4, b7, and BIn, respectively, but not on susceptible cotton plants.”
Claim · model-written summary
avrBIn elicited necrosis on cotton lines with BIn but not susceptible cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- constructs derived from avrB4, avrb7, and avrBIne0
- Gene
- avrBIne0
- Outcome
- necrosise0
- Direction
- observede0
- Comparator
- susceptible cotton plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A genotype-specific necrosis was observed 3 to 6 days post inoculation for constructs derived from avrB4, avrb7, and avrBIn on cotton lines containing the resistance (R) genes B4, b7, and BIn, respectively, but not on susceptible cotton plants.”
Claim · model-written summary
Expression of avr genes within A. tumefaciens produced no cotton necrosis.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- plasmids that direct expression of avr genes within A. tumefaciense0
- Interactor
- A. tumefaciens
Agrobacterium tumefaciense0other - Outcome
- necrosise0
- Direction
- No necrosise0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No necrosis was obtained with plasmids that direct expression of avr genes within A. tumefaciens”
t0“Cotton”
Claim · model-written summary
Targeting expressed Avr protein to the apoplast significantly reduced cotton responses.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- apoplaste0
- Treatment
- Addition of a signal peptide sequencee0
- Outcome
- responsese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of a signal peptide sequence into the avr constructs to target expressed Avr protein to the apoplast significantly reduced the responses.”
t0“Cotton”
Claim · model-written summary
The authors infer that intracellular Xcm Avr expression with corresponding cotton R genes causes host cell death.
- Taxon
- cottonGossypium hirsutume0
- Gene
- Xcm Avr proteinse0
- Outcome
- host cell deathe0
- Qualifier
- intracellular expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“intracellular expression of Xcm Avr proteins in cotton having the corresponding R gene causes specific host cell death.”
The same questions in other papers
Agrobacterium tumefaciens · disease · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- positive
transgenic · disease · Gossypium hirsutum (cotton)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.