Abstract
During the investigation Cucumber mosaic cucumovirus (CMV) was isolated from cucumber plants showing virus like symptoms depending on indirect enzyme linked immunosorbant assay (I-ELISA) and Chenopodium quinoa as local lesion host. Three isolates from the predominant plant growth-promoting rhizobacteria (PGPR) were isolated from cucumber plants rhizosphere, and identified morphologically and physiologically to be related to Bacillus subtilis, Pseudomonas fluorescens and Azotobacter chroococcum species. The bacterial liquid crude cultures (72 h of age) and their supernatants were tested for their ability to induce systemic resistance within cucumber plants (Cucumis sativus L. cultiver Beit Alpha) against CMV infection. Two types of treatment were carried out: (1) spraying of healthy cucumber plants (carrying 4–5 leaves) and challenging by mechanical CMV inoculation at time intervals (5–10 days), (2) irrigation, as healthy cucumber seeds were irrigated with 200 ml from each bacterial culture or their supernatants and inoculated with CMV 15 days post treatment. Data proved that best results were obtained by treatment of irrigation with the Azotobacter crude culture, as the number of symptomless plants were 11 out of 30 plants inoculated, followed by Pseudomonas treated plants which gave eight asymptomatic plants. The induced resistance was tested using I-ELISA and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) for the detection of CMV coat protein gene (cp), which proved that the mentioned symptomless plants were virus-free or with a low level of virus infection. Azotobacter treated plants giving virus-free results revealed the higher peroxidase and β-1,3-glucanase enzyme activities, 7 U/gm and 500 nktal/gm, respectively. Using gel electrophoresis and in comparison with control plants, a new protein band was detected in the protected cucumber plant extracts (molecular weight of about 30 KDa), assuming to be a plant pathogen related protein. Increase in growth measures was observed for Azotobacter and Pseudomonas treated cucumber plants, as the higher plant dry weights were 16.1 and 13.8 gm, respectively. Statistical lowest significant differences test (LSD) showed significant differences between Azotobacter and Pseudomonas results for biological data of plant dry weights.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
CMV was isolated from symptomatic cucumber plants.
- Taxon
- cucumber
Cucumis sativuse0 - Interactor
- Cucumber mosaic cucumovirus
cucumber mosaic cucumoviruse0pathogen - Outcome
- Cucumber mosaic cucumovirus (CMV) was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cucumber mosaic cucumovirus (CMV) was isolated from cucumber plants showing virus like symptoms”
Claim · model-written summary
The three cucumber rhizosphere isolates were related to three named bacterial species.
- Taxon
- cucumberCucumis sativuse0
- Interactor
- plant growth-promoting rhizobacteriae0symbiont
- Outcome
- identified morphologically and physiologically to be related to Bacillus subtilis, Pseudomonas fluorescens and Azotobacter chroococcum speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three isolates from the predominant plant growth-promoting rhizobacteria (PGPR) were isolated from cucumber plants rhizosphere, and identified morphologically and physiologically to be related to Bacillus subtilis, Pseudomonas fluorescens and Azotobacter chroococcum species”
Claim · model-written summary
Azotobacter crude culture irrigation yielded 11 symptomless cucumber plants among 30 inoculated.
- Taxon
- cucumberCucumis sativust0
- Treatment
- irrigation with the Azotobacter crude culturee0
- Interactor
- Azotobacter
Azotobactere0symbiont - Outcome
- number of symptomless plantse0
- Direction
- beste0
- Value
- 11 out of 30 plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“best results were obtained by treatment of irrigation with the Azotobacter crude culture, as the number of symptomless plants were 11 out of 30 plants inoculated”
t0“cucumber growth”
Claim · model-written summary
Pseudomonas treatment yielded eight asymptomatic cucumber plants.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Pseudomonas treatede0
- Interactor
- Pseudomonas
Pseudomonase0symbiont - Outcome
- asymptomatic plantse0
- Direction
- followed bye0
- Value
- eighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by Pseudomonas treated plants which gave eight asymptomatic plants”
t0“cucumber growth”
Claim · model-written summary
Symptomless cucumber plants were virus-free or had low CMV infection.
- Taxon
- cucumberCucumis sativust0
- Interactor
- Cucumber mosaic cucumoviruscucumber mosaic cucumovirust0pathogen
- Outcome
- virus-free or with a low level of virus infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mentioned symptomless plants were virus-free or with a low level of virus infection”
t0“Cucumber mosaic cucumovirus and promotion of cucumber growth”
Claim · model-written summary
Virus-free Azotobacter-treated cucumber plants showed higher peroxidase activity of 7 U/gm.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Azotobacter treatede0
- Interactor
- AzotobacterAzotobactere0symbiont
- Gene
- peroxidasee0
- Outcome
- peroxidasee0
- Direction
- highere0
- Value
- 7 U/gme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Azotobacter treated plants giving virus-free results revealed the higher peroxidase and β-1,3-glucanase enzyme activities, 7 U/gm and 500 nktal/gm, respectively”
t0“cucumber growth”
Claim · model-written summary
Virus-free Azotobacter-treated cucumber plants showed higher β-1,3-glucanase activity of 500 nktal/gm.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Azotobacter treatede0
- Interactor
- AzotobacterAzotobactere0symbiont
- Gene
- β-1,3-glucanasee0
- Outcome
- β-1,3-glucanase enzyme activitiese0
- Direction
- highere0
- Value
- 500 nktal/gme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Azotobacter treated plants giving virus-free results revealed the higher peroxidase and β-1,3-glucanase enzyme activities, 7 U/gm and 500 nktal/gm, respectively”
t0“cucumber growth”
Claim · model-written summary
Protected cucumber extracts showed a new approximately 30 KDa protein band relative to controls.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- protectede0
- Outcome
- new protein bande0
- Value
- about 30 KDae0
- Comparator
- control plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in comparison with control plants, a new protein band was detected in the protected cucumber plant extracts (molecular weight of about 30 KDa)”
Claim · model-written summary
Azotobacter-treated cucumber plants had increased dry weight of 16.1 gm.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Azotobactere0
- Interactor
- AzotobacterAzotobactere0symbiont
- Outcome
- plant dry weightse0
- Direction
- Increasee0
- Value
- 16.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increase in growth measures was observed for Azotobacter and Pseudomonas treated cucumber plants, as the higher plant dry weights were 16.1 and 13.8 gm, respectively”
Claim · model-written summary
Pseudomonas-treated cucumber plants had increased dry weight of 13.8 gm.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Pseudomonase0
- Interactor
- PseudomonasPseudomonase0symbiont
- Outcome
- plant dry weightse0
- Direction
- Increasee0
- Value
- 13.8 gme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increase in growth measures was observed for Azotobacter and Pseudomonas treated cucumber plants, as the higher plant dry weights were 16.1 and 13.8 gm, respectively”
Claim · model-written summary
Azotobacter and Pseudomonas treatments differed significantly in cucumber dry weight.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Azotobactere0
- Interactor
- AzotobacterAzotobactere0symbiont
- Second interactor
- PseudomonasPseudomonase0symbiont
- Outcome
- plant dry weightse0
- Comparator
- Pseudomonase0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant differences between Azotobacter and Pseudomonas results for biological data of plant dry weights”
t0“cucumber growth”
The same questions in other papers
Azotobacter · growth · Cucumis sativus (cucumber)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Pseudomonas · growth · Cucumis sativus (cucumber)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.