Paper · 2008 · Environment Control in Biology

Supplementary Ultraviolet Radiation B Together with Blue Light at Night Increased Quercetin Content and Flavonol Synthase Gene Expression in Leaf Lettuce (Lactuca sativa L.)

Masanori EBISAWA; Kazuhiro SHOJI; Mieko KATO; et al.

5
claims
7
verbatim quotes
80
citations
4
research areas
✓
Open access

Abstract

Establishment of an effective supplementary lighting procedure is necessary to increase the value of leaf lettuce grown using a hydroponic method involving a low production cost. In leaf lettuce extracts, quercetin, one of the flavonoids, was isolated and identified. It was investigated that quercetin has important functions that can be used as a dietary supplement. Flavonol synthase (FLS) is a key enzyme involved in quercetin biosynthesis, catalyzes the conversion of dihydroquercetin to quercetin. Therefore, we determined the sequence of the flavonol synthase gene (FLS) in red leaf lettuce. We harvested leaf lettuce grown using supplementary light sources, such as ultraviolet radiation B (UV-B), ultraviolet radiation A, blue, and red lamps during the night. It is noteworthy that FLS expression and the quercetin content were particularly increased to a greater extent in young leaves than in mature leaves when UV-B and blue light were used simultaneously at night. We suggest that UV-B with blue light is used simultaneously at night for producing leaf lettuce with high quercetin content.

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Quercetin was isolated and identified from leaf lettuce extracts.

Taxon
leaf lettucee0
Outcome
quercetin, one of the flavonoidse0

Verbatim

e0

“In leaf lettuce extracts, quercetin, one of the flavonoids, was isolated and identified.”

✓ located verbatim in the source · abstract, characters 179–267 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The FLS sequence was determined in red leaf lettuce.

Taxon
red leaf lettucee0
Gene
flavonol synthase gene (FLS)e0
Outcome
sequence of the flavonol synthase gene (FLS)e0

Not reportedComparatorValue

Verbatim

e0

“we determined the sequence of the flavonol synthase gene (FLS) in red leaf lettuce”

✓ located verbatim in the source · abstract, characters 515–597 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Concurrent UV-B and blue light at night increased FLS expression more in young than mature lettuce leaves.

Taxon
Leaf Lettucet0
Organ / stage
young leavese0
Treatment
UV-B and blue lighte0
Gene
FLSe0
Outcome
FLS expressione0
Direction
increasede0
Comparator
mature leavese0

Verbatim

e0

“FLS expression and the quercetin content were particularly increased to a greater extent in young leaves than in mature leaves when UV-B and blue light were used simultaneously at night”

✓ located verbatim in the source · abstract, characters 791–976 · show in text ↑
t0

“Leaf Lettuce (Lactuca sativa L.)”

✓ located verbatim in the source · title, characters 141–173 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Concurrent UV-B and blue light at night increased quercetin content more in young than mature lettuce leaves.

Taxon
Leaf Lettucet0
Organ / stage
young leavese0
Treatment
UV-B and blue lighte0
Outcome
quercetin contente0
Direction
increasede0
Comparator
mature leavese0

Verbatim

e0

“FLS expression and the quercetin content were particularly increased to a greater extent in young leaves than in mature leaves when UV-B and blue light were used simultaneously at night”

✓ located verbatim in the source · abstract, characters 791–976 · show in text ↑
t0

“Leaf Lettuce (Lactuca sativa L.)”

✓ located verbatim in the source · title, characters 141–173 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest using concurrent UV-B and blue light at night to produce lettuce with high quercetin content.

Taxon
leaf lettucee0
Outcome
UV-B with blue light is used simultaneously at night for producing leaf lettuce with high quercetin contente0

Verbatim

e0

“We suggest that UV-B with blue light is used simultaneously at night for producing leaf lettuce with high quercetin content.”

✓ located verbatim in the source · abstract, characters 978–1102 · show in text ↑

radiation · molecular: metabolites · Lactuca sativa (garden lettuce)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. no effect
  3. no effect

3 papers more in this group

See all 7 papers →

light · molecular: expression · Lactuca sativa (garden lettuce)Positive and negative results

12 papers: 9 positive, 3 negative or no effect

8 papers in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. no effect
  3. no effect

8 papers more in this group

See all 12 papers →

uv · molecular: metabolites · Lactuca sativa (garden lettuce)Positive and negative results

13 papers: 12 positive, 1 negative or no effect

11 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

light · molecular: metabolites · Lactuca sativa (garden lettuce)Positive and negative results

52 papers: 42 positive, 10 negative or no effect

41 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

48 papers more in this group

See all 52 papers →

radiation · molecular: expression · Lactuca sativa (garden lettuce)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.