Abstract
Four cDNA clones (SlArf/Xyl1-4) encoding α-l-arabinofuranosidase/β-xylosidase belonging to glycoside hydrolase family 3 were obtained from tomato (Solanum lycopersicum) fruit. SlArf/Xyl1 was expressed in various organs. Its level was particularly high in flower and leaves but low in fruit. SlArf/Xyl3 was highly expressed in flower. On the contrary, SlArf/Xyl2 and 4 were expressed in early developmental stage in various organs. Comparison with SlArf/Xyl4, SlArf/Xyl2 expression was observed in earlier stages. The active recombinant proteins were obtained by using BY-2 tobacco (Nicotiana tabacum) suspension cultured cells. The SlArf/Xyl1 and 2 recombinant proteins showed a bi-functional activity of α-l-arabinofuranosidase/β-xylosidase while the SlArf/Xyl4 protein possessed a β-xylosidase activity predominantly. Neither enzyme activities were detected for the SlArf/Xyl3 protein under the same conditions. Although SlArf/Xyl2 possessed a bi-functional activity, it preferentially hydrolyzed arabinosyl residues from tomato hemicellulosic polysaccharides. Antisense suppression of SlArf/Xyl2 resulted in no apparent changes in the enzyme activities, monosaccharide composition or fruit phenotype. Increment of a family 51 α-l-arabinofuranosidase expression rather than that of family 3 resulted in a restoring the activity in SlArf/Xyl2-suppressed fruit. The ability of recombinant SlArf/Xyl2 to hydrolyze both arabinan and arabinoxylan is nearly identical to that of α-l-arabinofuranosidases belonging to family 51. Our results suggested that BY-2 cells are a useful expression system for obtaining active cell wall hydrolyzing enzymes. In addition, an α-l-arabinofuranosidase activity derived from SlArf/Xyl2 would be essential in young organ development and the action of the enzyme could be restored by the other enzyme belonging to a different family under a defective condition.
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Claims 14
Claim · model-written summary
Four SlArf/Xyl cDNA clones were obtained from tomato fruit.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- fruite0
- Gene
- SlArf/Xyl1-4e0
- Outcome
- Four cDNA clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four cDNA clones (SlArf/Xyl1-4) encoding α-l-arabinofuranosidase/β-xylosidase belonging to glycoside hydrolase family 3 were obtained from tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl1 expression was highest in flower and leaves and low in fruit.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- flower and leavese0
- Gene
- SlArf/Xyl1e0
- Outcome
- Its levele0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlArf/Xyl1 was expressed in various organs. Its level was particularly high in flower and leaves but low in fruit”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl3 was highly expressed in flower.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- flowere0
- Gene
- SlArf/Xyl3e0
- Outcome
- expressede0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlArf/Xyl3 was highly expressed in flower”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl2 and 4 were expressed in early developmental stages, with SlArf/Xyl2 earlier than SlArf/Xyl4.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- early developmental stagee0
- Gene
- SlArf/Xyl2 and 4e0
- Outcome
- expressede0
- Direction
- earliere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlArf/Xyl2 and 4 were expressed in early developmental stage in various organs. Comparison with SlArf/Xyl4, SlArf/Xyl2 expression was observed in earlier stages”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
BY-2 tobacco cells yielded active recombinant proteins.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- BY-2e0
- Outcome
- active recombinant proteinse0
- Value
- activee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The active recombinant proteins were obtained by using BY-2 tobacco (Nicotiana tabacum) suspension cultured cells”
Claim · model-written summary
SlArf/Xyl1 and 2 proteins had bifunctional activity.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlArf/Xyl1 and 2e0
- Outcome
- bi-functional activity of α-l-arabinofuranosidase/β-xylosidasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SlArf/Xyl1 and 2 recombinant proteins showed a bi-functional activity of α-l-arabinofuranosidase/β-xylosidase”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl4 predominantly had β-xylosidase activity.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlArf/Xyl4e0
- Outcome
- β-xylosidase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SlArf/Xyl4 protein possessed a β-xylosidase activity predominantly”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl3 had neither assayed enzyme activity.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlArf/Xyl3e0
- Outcome
- enzyme activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither enzyme activities were detected for the SlArf/Xyl3 protein under the same conditions”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
SlArf/Xyl2 preferentially hydrolyzed arabinosyl residues from tomato hemicellulose.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlArf/Xyl2e0
- Outcome
- hydrolyzed arabinosyl residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlArf/Xyl2 possessed a bi-functional activity, it preferentially hydrolyzed arabinosyl residues from tomato hemicellulosic polysaccharides”
Claim · model-written summary
SlArf/Xyl2 suppression produced no apparent changes in enzyme activity, monosaccharide composition or fruit phenotype.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Antisense suppression of SlArf/Xyl2e0
- Gene
- SlArf/Xyl2e0
- Outcome
- enzyme activities, monosaccharide composition or fruit phenotypee0
- Direction
- no apparent changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense suppression of SlArf/Xyl2 resulted in no apparent changes in the enzyme activities, monosaccharide composition or fruit phenotype”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
Increased family 51 enzyme expression restored activity in suppressed fruit.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- fruite0
- Gene
- family 51 α-l-arabinofuranosidasee0
- Outcome
- restoring the activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increment of a family 51 α-l-arabinofuranosidase expression rather than that of family 3 resulted in a restoring the activity in SlArf/Xyl2-suppressed fruit”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
Recombinant SlArf/Xyl2 hydrolyzed arabinan and arabinoxylan nearly identically to family 51 arabinofuranosidases.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlArf/Xyl2e0
- Outcome
- hydrolyze both arabinan and arabinoxylane0
- Comparator
- α-l-arabinofuranosidases belonging to family 51e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ability of recombinant SlArf/Xyl2 to hydrolyze both arabinan and arabinoxylan is nearly identical to that of α-l-arabinofuranosidases belonging to family 51”
e1“tomato (Solanum lycopersicum) fruit”
Claim · model-written summary
Authors suggested BY-2 cells are useful for obtaining active cell-wall hydrolases.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- BY-2e0
- Outcome
- obtaining active cell wall hydrolyzing enzymese0
- Value
- activee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BY-2 cells are a useful expression system for obtaining active cell wall hydrolyzing enzymes”
e1“BY-2 tobacco (Nicotiana tabacum) suspension cultured cells”
Claim · model-written summary
Authors inferred SlArf/Xyl2 activity is essential in young organ development and can be restored by a different enzyme family.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- young organe0
- Gene
- SlArf/Xyl2e0
- Outcome
- young organ developmente0
- Qualifier
- could be restored by the other enzyme belonging to a different familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an α-l-arabinofuranosidase activity derived from SlArf/Xyl2 would be essential in young organ development and the action of the enzyme could be restored by the other enzyme belonging to a different family”
e1“tomato (Solanum lycopersicum) fruit”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.