Abstract
Summary Tomato plants ( Lycopersicon esculentum Mill. cv. Ailsa Craig) were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes, based on a cDNA isolated from a tomato fruit library. PE enzyme activity in ripe fruit of these plants was reduced by up to 93%, as a result of inhibition of PE mRNA accumulation from an endogenous gene, or genes. No major differences in fruit development and ripening were apparent, although pectin remained more heavily esterified (methylated) at all stages of fruit development, thus confirming that PE has a role in pectin de‐esterification in vivo . PE enzyme activity was unaffected in leaves or roots of the transformants, although antisense RNA transcripts were detected. This observation, and immunological differences between PE isoenzymes in leaves and roots compared to fruit, indicate differential expression of PE isoenzymes in these organs.
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Claims 8
Claim · model-written summary
Antisense lines had up to 93% less pectin esterase activity in ripe fruit.
- Taxon
- Tomato plants
Solanum lycopersicumt0 - Organ / stage
- ripe fruite0
- Treatment
- antisense RNA genese1
- Gene
- PEe0
- Outcome
- PE enzyme activity in ripe fruite0
- Direction
- reducede0
- Value
- 93%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PE enzyme activity in ripe fruit of these plants was reduced by up to 93%, as a result of inhibition of PE mRNA accumulation from an endogenous gene, or genes”
t0“Summary Tomato plants”
e1“were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes”
Claim · model-written summary
Antisense suppression inhibited endogenous PE mRNA accumulation.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Treatment
- antisense RNA genese1
- Gene
- PEe0
- Outcome
- PE mRNA accumulatione0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as a result of inhibition of PE mRNA accumulation from an endogenous gene, or genes”
t0“Summary Tomato plants”
e1“were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes”
Claim · model-written summary
Fruit development and ripening showed no major apparent differences.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Treatment
- antisense RNA genese1
- Outcome
- fruit development and ripeninge0
- Direction
- No major differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No major differences in fruit development and ripening were apparent”
t0“Summary Tomato plants”
e1“were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes”
Claim · model-written summary
Fruit pectin remained more heavily esterified throughout development.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Organ / stage
- fruite0
- Treatment
- antisense RNA genese1
- Outcome
- pectine0
- Direction
- more heavily esterifiede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pectin remained more heavily esterified (methylated) at all stages of fruit development”
t0“Summary Tomato plants”
e1“were transformed with chimaeric pectin esterase (PE; EC 3.1.1.11) antisense RNA genes”
Claim · model-written summary
PE activity was unaffected in transformant leaves and roots.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Organ / stage
- leaves or rootse0
- Treatment
- transformantse0
- Outcome
- PE enzyme activitye0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PE enzyme activity was unaffected in leaves or roots of the transformants, although antisense RNA transcripts were detected”
t0“Summary Tomato plants”
Claim · model-written summary
Antisense RNA transcripts were detected in leaves and roots.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Organ / stage
- leaves or rootse0
- Outcome
- antisense RNA transcripts were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PE enzyme activity was unaffected in leaves or roots of the transformants, although antisense RNA transcripts were detected”
t0“Summary Tomato plants”
Claim · model-written summary
PE isoenzymes differed immunologically between leaves and roots versus fruit.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Organ / stage
- leaves and rootse0
- Outcome
- immunological differences between PE isoenzymese0
- Comparator
- fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“immunological differences between PE isoenzymes in leaves and roots compared to fruit”
t0“Summary Tomato plants”
Claim · model-written summary
The findings indicate differential organ expression of PE isoenzymes.
- Taxon
- Tomato plantsSolanum lycopersicumt0
- Organ / stage
- these organse0
- Gene
- PEe0
- Outcome
- differential expression of PE isoenzymese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicate differential expression of PE isoenzymes in these organs”
t0“Summary Tomato plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.