Abstract
The uptake, translocation, and compartmentalization of Fe are essential for plant cell function and life cycle. Despite rapid progress in our understanding of Fe homeostasis in plants, Fe transport from the cytoplasm to mitochondria was, until recently, poorly understood. The screening of 3,993 mutant lines for symptoms of Fe deficiency resulted in the identification and characterization of a major mitochondrial Fe transporter (MIT) in rice. MIT was found to localize to mitochondria and to complement the growth of a yeast strain defective in mitochondrial Fe transport. The knock-out of MIT resulted in a lethal phenotype, and in knock-down plants, several agronomic characteristics were compromised, such as plant height, average number of tillers, days to flower, fertility, and yield. Changes in the expression of genes involved in Fe transport suggested a disturbance of cellular Fe transport. Furthermore, the mitochondrial Fe concentration and the activity of the mitochondrial Fe-S enzyme aconitase were significantly reduced compared with wild-type plants. The identification of MIT is a significant advance in the field of plant Fe nutrition and should facilitate the cloning of paralogs from other plant species.
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Claims 9
Claim · model-written summary
Screening rice mutants identified a major mitochondrial iron transporter, MIT.
- Taxon
- rice
Oryza sativae0 - Gene
- MITe0
- Outcome
- major mitochondrial Fe transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification and characterization of a major mitochondrial Fe transporter (MIT) in rice”
Claim · model-written summary
MIT localized to mitochondria.
- Taxon
- riceOryza sativae1
- Gene
- MITe0
- Outcome
- localize to mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIT was found to localize to mitochondria”
e1“in rice”
Claim · model-written summary
MIT complemented growth of yeast defective in mitochondrial iron transport.
- Taxon
- riceOryza sativae2
- Gene
- MITe1
- Outcome
- complement the growth of a yeast strain defective in mitochondrial Fe transporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to complement the growth of a yeast strain defective in mitochondrial Fe transport”
e1“MIT was found to localize to mitochondria”
e2“in rice”
Claim · model-written summary
MIT knock out resulted in a lethal phenotype in rice.
- Taxon
- riceOryza sativae1
- Treatment
- knock-out of MITe0
- Gene
- MITe0
- Outcome
- lethal phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The knock-out of MIT resulted in a lethal phenotype”
e1“in rice”
Claim · model-written summary
MIT knock down compromised plant height, tiller number and days to flower.
- Taxon
- riceOryza sativae1
- Treatment
- knock-downe0
- Outcome
- plant height, average number of tillers, days to flowere0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in knock-down plants, several agronomic characteristics were compromised, such as plant height, average number of tillers, days to flower, fertility, and yield”
e1“in rice”
Claim · model-written summary
MIT knock down compromised fertility and yield.
- Taxon
- riceOryza sativae1
- Treatment
- knock-downe0
- Outcome
- fertility, and yielde0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in knock-down plants, several agronomic characteristics were compromised, such as plant height, average number of tillers, days to flower, fertility, and yield”
e1“in rice”
Claim · model-written summary
Expression changes in iron transport genes suggested disturbed cellular iron transport.
- Taxon
- riceOryza sativae1
- Gene
- genes involved in Fe transporte0
- Outcome
- cellular Fe transporte0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in the expression of genes involved in Fe transport suggested a disturbance of cellular Fe transport”
e1“in rice”
Claim · model-written summary
Mitochondrial iron concentration was significantly reduced compared with wild type rice.
- Taxon
- riceOryza sativae2
- Treatment
- knock-downe1
- Outcome
- mitochondrial Fe concentratione0
- Direction
- reducede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondrial Fe concentration and the activity of the mitochondrial Fe-S enzyme aconitase were significantly reduced compared with wild-type plants”
e1“in knock-down plants”
e2“in rice”
Claim · model-written summary
Mitochondrial aconitase activity was significantly reduced compared with wild type rice.
- Taxon
- riceOryza sativae2
- Treatment
- knock-downe1
- Gene
- aconitasee0
- Outcome
- activity of the mitochondrial Fe-S enzyme aconitasee0
- Direction
- reducede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondrial Fe concentration and the activity of the mitochondrial Fe-S enzyme aconitase were significantly reduced compared with wild-type plants”
e1“in knock-down plants”
e2“in rice”
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