Abstract
Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content. The damage was assessed in terms of seedling injury on the eighth day of growth. An increase in seedling injury is clearly seen, associated with a parallel increase in the peroxidase activity. There is a concomitant decrease in the content of total peroxides. Both these post-irradiation treatments potentiate the O2-independent component of seedling injury, irrespective of the seed moisture content. Analysis of the peroxidase activity in the seedlings using non-denaturing polyacrylamide gel electrophoresis reveals that two additional bands appear with the post-irradiation oxic damage. Radioprotection against this damage by caffeine, heat shock and O2-free post-irradiation hydration is accompanied by the disappearance of these two additional bands. However there is no appearance of the two additional bands in the peroxidase family even though the enzyme activity is substantially increased due to the action of caffeine and/or heat shock on the post-irradiation O2-independent pathway of damage. The probable mechanisms of radioprotection are discussed in this paper.
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Claims 11
Claim · model-written summary
Wet heat shock protected low-moisture barley seeds from oxygen-dependent post-irradiation damage.
- Taxon
- Barley
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Hordeum vulgaret0 - Organ / stage
- seedse0
- Treatment
- Wet heat shock (60 degrees C, 90 s)e0
- Outcome
- post-irradiation O2-dependent damagee0
- Direction
- radioprotectione0
- Qualifier
- approximately 3.5% moisture contente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content”
t0“Barley Seeds”
Claim · model-written summary
Caffeine protected low-moisture barley seeds from oxygen-dependent post-irradiation damage.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- seedse0
- Treatment
- caffeine (3.8 X 10(-4) M)e0
- Outcome
- post-irradiation O2-dependent damagee0
- Direction
- radioprotectione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content”
t0“Barley Seeds”
Claim · model-written summary
Seedling injury rose with peroxidase activity.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- peroxidasee0
- Outcome
- seedling injurye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase in seedling injury is clearly seen, associated with a parallel increase in the peroxidase activity”
t0“Barley Seeds”
Claim · model-written summary
Total peroxide content decreased alongside post-irradiation injury.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- content of total peroxidese0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There is a concomitant decrease in the content of total peroxides.”
t0“Barley Seeds”
Claim · model-written summary
Wet heat shock potentiated the oxygen-independent component of seedling injury irrespective of moisture content.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Wet heat shock (60 degrees C, 90 s)e1
- Outcome
- O2-independent component of seedling injurye0
- Direction
- potentiatee0
- Qualifier
- irrespective of the seed moisture contente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both these post-irradiation treatments potentiate the O2-independent component of seedling injury, irrespective of the seed moisture content”
e1“Wet heat shock (60 degrees C, 90 s)”
t0“Barley Seeds”
Claim · model-written summary
Caffeine potentiated the oxygen-independent component of seedling injury irrespective of moisture content.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- caffeine (3.8 X 10(-4) M)e1
- Outcome
- O2-independent component of seedling injurye0
- Direction
- potentiatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both these post-irradiation treatments potentiate the O2-independent component of seedling injury, irrespective of the seed moisture content”
e1“caffeine (3.8 X 10(-4) M)”
t0“Barley Seeds”
Claim · model-written summary
Two additional peroxidase bands appeared with oxygen-dependent damage.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- two additional bands appeare0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two additional bands appear with the post-irradiation oxic damage”
t0“Barley Seeds”
Claim · model-written summary
Caffeine radioprotection coincided with disappearance of the additional bands.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- caffeinee0
- Outcome
- disappearance of these two additional bandse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Radioprotection against this damage by caffeine, heat shock and O2-free post-irradiation hydration is accompanied by the disappearance of these two additional bands”
t0“Barley Seeds”
Claim · model-written summary
Heat shock radioprotection coincided with disappearance of the additional bands.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- heat shocke0
- Outcome
- disappearance of these two additional bandse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Radioprotection against this damage by caffeine, heat shock and O2-free post-irradiation hydration is accompanied by the disappearance of these two additional bands”
t0“Barley Seeds”
Claim · model-written summary
Oxygen-free hydration radioprotection coincided with disappearance of the additional bands.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- O2-free post-irradiation hydratione0
- Outcome
- disappearance of these two additional bandse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Radioprotection against this damage by caffeine, heat shock and O2-free post-irradiation hydration is accompanied by the disappearance of these two additional bands”
t0“Barley Seeds”
Claim · model-written summary
Caffeine or heat shock raised peroxidase activity along the oxygen-independent damage pathway without the two extra bands.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- no appearance of the two additional bands in the peroxidase familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no appearance of the two additional bands in the peroxidase family even though the enzyme activity is substantially increased due to the action of caffeine and/or heat shock on the post-irradiation O2-independent pathway of damage”
t0“Barley Seeds”
The same questions in other papers
caffeine · pest · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →caffeine · crop injury · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- negative
heat · crop injury · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- positive
heat · pest · Hordeum vulgare (barley)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.