Paper · 1979 · Canadian Journal of Botany

Influence of plant development stage, temperature, and plant hormones on chromosome doubling of barley haploids using colchicine

J. Thiebaut; K. J. Kasha; A. Tsai

11
claims
25
verbatim quotes
23
citations
3
research areas
—
Not open access

Abstract

Varying colchicine treatments in the direction of earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA) was without effect on percent of surviving plants that set seed. However, all three factors did increase the extent of doubling within the plant. This increase in size of doubled sectors suggests that the addition of BA and the shift to higher temperatures stimulate cell divisions in meristematic regions. The addition of gibberellic acid (GA 3 ), on the other hand, led to a decrease in size of doubled sectors. Comparisons among experiments, although nonorthogonal for genotype, confirmed that GA 3 increased plant survival after colchicine treatment and indicated that BA had a similar effect at 20 to 22 °C. However, the combination of high temperature (32 °C) and addition of BA increased plant lethality following colchicine treatment.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Earlier developmental stage had no effect on the fraction of surviving plants setting seed.

Treatment
earlier developmental stagese0
Outcome
percent of surviving plants that set seede0
Direction
without effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA) was without effect on percent of surviving plants that set seed”

✓ located verbatim in the source · abstract, characters 50–204 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Higher temperatures had no effect on the fraction of surviving plants setting seed.

Treatment
higher temperaturese0
Outcome
percent of surviving plants that set seede0
Direction
without effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA) was without effect on percent of surviving plants that set seed”

✓ located verbatim in the source · abstract, characters 50–204 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Adding BA had no effect on the fraction of surviving plants setting seed.

Treatment
addition of N-6-benzyl adenine (BA)e0
Outcome
percent of surviving plants that set seede0
Direction
without effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“addition of N-6-benzyl adenine (BA) was without effect on percent of surviving plants that set seed”

✓ located verbatim in the source · abstract, characters 105–204 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Earlier developmental stage increased the extent of doubling within the plant.

Treatment
earlier developmental stagest1
Outcome
extent of doubling within the plante0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“all three factors did increase the extent of doubling within the plant”

✓ located verbatim in the source · abstract, characters 215–285 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
t1

“earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA)”

✓ located verbatim in the source · abstract, characters 50–140 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher temperatures increased the extent of doubling within the plant.

Treatment
higher temperaturest1
Outcome
extent of doubling within the plante0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“all three factors did increase the extent of doubling within the plant”

✓ located verbatim in the source · abstract, characters 215–285 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
t1

“earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA)”

✓ located verbatim in the source · abstract, characters 50–140 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BA addition increased the extent of doubling within the plant.

Treatment
addition of N-6-benzyl adenine (BA)t1
Outcome
extent of doubling within the plante0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“all three factors did increase the extent of doubling within the plant”

✓ located verbatim in the source · abstract, characters 215–285 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
t1

“earlier developmental stages, higher temperatures, and addition of N-6-benzyl adenine (BA)”

✓ located verbatim in the source · abstract, characters 50–140 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

BA and higher temperatures were inferred to stimulate meristematic cell division.

Treatment
addition of BA and the shift to higher temperaturese0
Outcome
cell divisions in meristematic regionse0
Direction
stimulatee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“addition of BA and the shift to higher temperatures stimulate cell divisions in meristematic regions”

✓ located verbatim in the source · abstract, characters 346–446 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Gibberellic acid addition decreased the size of doubled sectors.

Treatment
addition of gibberellic acid (GA 3 )e0
Outcome
size of doubled sectorse0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of gibberellic acid (GA 3 ), on the other hand, led to a decrease in size of doubled sectors”

✓ located verbatim in the source · abstract, characters 448–553 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GA 3 increased survival after colchicine treatment.

Treatment
GA 3e0
Outcome
plant survivale0
Direction
increasede0
Qualifier
after colchicine treatmente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GA 3 increased plant survival after colchicine treatment”

✓ located verbatim in the source · abstract, characters 638–694 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BA increased survival at 20 to 22 °C after colchicine treatment.

Treatment
BAe0
Outcome
similar effecte0
Qualifier
20 to 22 °Ce0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“BA had a similar effect at 20 to 22 °C”

✓ located verbatim in the source · abstract, characters 714–752 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

BA with 32 °C increased lethality after colchicine treatment.

Treatment
high temperature (32 °C) and addition of BAe0
Outcome
plant lethalitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the combination of high temperature (32 °C) and addition of BA increased plant lethality following colchicine treatment”

✓ located verbatim in the source · abstract, characters 763–882 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 96–102 · show in text ↑

N-benzyladenine · survival · Hordeum vulgare (barley)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

gibberellin A3 · growth · Hordeum vulgare (barley)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

heat · pest · Hordeum vulgare (barley)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

1 paper in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.