Abstract
Despite the obligatory role of ethylene in climacteric fruit ripening and the identification of 77 ethylene response factors (ERFs) in the tomato (Solanum lycopersicum) genome, the role of few ERFs has been validated in the ripening process. Here, using a comprehensive morpho-physiological, molecular, and biochemical approach, we demonstrate the regulatory role of ERF D7 (SlERF.D7) in tomato fruit ripening. SlERF.D7 expression positively responded to exogenous ethylene and auxin treatments, most likely in a ripening inhibitor-independent manner. SlERF.D7 overexpression (OE) promoted ripening, and its silencing had the opposite effect. Alterations in its expression modulated ethylene production, pigment accumulation, and fruit firmness. Consistently, genes involved in ethylene biosynthesis and signaling, lycopene biosynthesis, and cell wall loosening were upregulated in the OE lines and downregulated in RNAi lines. These transgenic lines also accumulated altered levels of indole-3-acetic acid at late-breaker stages. A positive association between auxin response factor 2 (ARF2) paralog's transcripts and SlERF.D7 mRNA levels and that SlARF2A and SlARF2B are direct targets of SlERF.D7 underpinned the perturbed auxin-ethylene crosstalk for the altered ripening program observed in the transgenic fruits. Overall, this study uncovers that SlERF.D7 positively regulates SlARF2A/B abundance to amalgamate auxin and ethylene signaling pathways for controlling tomato fruit ripening.
Abstract from the paper’s OpenAlex record; open copy licensed public-domain. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
SlERF.D7 expression responded positively to ethylene and auxin treatments.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- exogenous ethylene and auxin treatmentse0
- Gene
- SlERF.D7e0
- Outcome
- SlERF.D7 expressione0
- Direction
- positively respondede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlERF.D7 expression positively responded to exogenous ethylene and auxin treatments”
t0“tomato”
Claim · model-written summary
SlERF.D7 overexpression promoted tomato ripening.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SlERF.D7 overexpression (OE)e0
- Gene
- SlERF.D7e0
- Outcome
- ripeninge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlERF.D7 overexpression (OE) promoted ripening”
t0“tomato”
Claim · model-written summary
SlERF.D7 silencing had the opposite effect on ripening.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- its silencinge0
- Outcome
- ripeninge0
- Direction
- opposite effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlERF.D7 overexpression (OE) promoted ripening, and its silencing had the opposite effect”
t0“tomato”
Claim · model-written summary
Altering SlERF.D7 expression modulated ethylene production, pigment accumulation, and fruit firmness.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- ethylene production, pigment accumulation, and fruit firmnesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alterations in its expression modulated ethylene production, pigment accumulation, and fruit firmness”
t0“tomato”
Claim · model-written summary
Ethylene biosynthesis and signaling genes were upregulated in OE lines.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- OE linese0
- Outcome
- genes involved in ethylene biosynthesis and signaling, lycopene biosynthesis, and cell wall looseninge0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes involved in ethylene biosynthesis and signaling, lycopene biosynthesis, and cell wall loosening were upregulated in the OE lines and downregulated in RNAi lines”
t0“tomato”
Claim · model-written summary
The same genes were downregulated in RNAi lines.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- RNAi linese0
- Outcome
- genes involved in ethylene biosynthesis and signaling, lycopene biosynthesis, and cell wall looseninge0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes involved in ethylene biosynthesis and signaling, lycopene biosynthesis, and cell wall loosening were upregulated in the OE lines and downregulated in RNAi lines”
t0“tomato”
Claim · model-written summary
Transgenic lines had altered indole-3-acetic acid levels at late breaker stage.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- transgenic linese0
- Organ / stage
- late-breaker stagese0
- Outcome
- levels of indole-3-acetic acide0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These transgenic lines also accumulated altered levels of indole-3-acetic acid at late-breaker stages”
t0“tomato”
Claim · model-written summary
ARF2 paralog transcripts were positively associated with SlERF.D7 mRNA levels.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlERF.D7e0
- Outcome
- auxin response factor 2 (ARF2) paralog's transcriptse0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A positive association between auxin response factor 2 (ARF2) paralog's transcripts and SlERF.D7 mRNA levels”
t0“tomato”
Claim · model-written summary
SlARF2A and SlARF2B are direct SlERF.D7 targets.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlERF.D7e0
- Outcome
- SlARF2A and SlARF2B are direct targetse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlARF2A and SlARF2B are direct targets of SlERF.D7”
t0“tomato”
The same questions in other papers
ethene · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
15 papers in the same direction as this paper · 14 in the opposite direction
- no effect
- no effect
- negative
26 papers more in this group
See all 30 papers →class · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
17 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- no effect
- no effect
21 papers more in this group
See all 25 papers →loss of function · quality: postharvest · Solanum lycopersicum (tomato)Positive and negative results
32 papers in the same direction as this paper · 21 in the opposite direction
- positive
- negative
- negative
50 papers more in this group
See all 54 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.