Abstract
Adenosine nucleosidase (adenosine ribohydrolase, EC 3.2.2.7) which catalyzes the deribosylation of N(6)-(Delta(2)-isopentenyl)adenosine and adenosine to form the corresponding bases was partially purified from wheat germ. This enzyme (molecular weight 59,000 +/- 3,000) deribosylates the ribonucleosides at an optimum pH of 4.7 K(m) values for the cytokinin nucleoside and adenosine are 2.38 and 1.43 micromolar, respectively, in 50 millimolar Tris-citrate buffer (pH 4.7) at 30 C. The presence of adenosine and other cytokinin nucleosides inhibited the hydrolysis of N(6)-(Delta(2)-isopentenyl)adenosine but this reaction was insensitive to guanosine, uridine, or 3'-deoxyadenosine. It is hypothesized that an adequate level of "active cytokinin" in plant cells may be provided through the deribosylation of cytokinin riboside in concert with other cytokinin metabolic enzymes.
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Claims 8
Claim · model-written summary
Adenosine nucleosidase, which deribosylates N6-(Delta2-isopentenyl)adenosine and adenosine to the corresponding bases, was partially purified from wheat germ.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- Adenosine nucleosidasee0
- Outcome
- deribosylation of N(6)-(Delta(2)-isopentenyl)adenosine and adenosinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“Adenosine nucleosidase (adenosine ribohydrolase, EC 3.2.2.7) which catalyzes the deribosylation of N(6)-(Delta(2)-isopentenyl)adenosine and adenosine to form the corresponding bases was partially purified from wheat germ”
Claim · model-written summary
The wheat germ adenosine nucleosidase has a molecular weight of 59,000 +/- 3,000.
- Taxon
- WheatTriticum aestivumt0
- Gene
- enzymee0
- Outcome
- molecular weighte0
- Value
- 59,000 +/- 3,000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“This enzyme (molecular weight 59,000 +/- 3,000)”
Claim · model-written summary
The enzyme deribosylates the ribonucleosides at an optimum pH of 4.7.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- optimum pHe0
- Value
- 4.7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“deribosylates the ribonucleosides at an optimum pH of 4.7”
Claim · model-written summary
The Km of the enzyme for the cytokinin nucleoside is 2.38 micromolar in 50 mM Tris-citrate buffer (pH 4.7) at 30 C.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- K(m) valuese0
- Value
- 2.38e0
- Qualifier
- the cytokinin nucleosidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“K(m) values for the cytokinin nucleoside and adenosine are 2.38 and 1.43 micromolar, respectively”
Claim · model-written summary
The Km of the enzyme for adenosine is 1.43 micromolar in 50 mM Tris-citrate buffer (pH 4.7) at 30 C.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- K(m) valuese0
- Value
- 1.43 micromolare0
- Qualifier
- adenosinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“K(m) values for the cytokinin nucleoside and adenosine are 2.38 and 1.43 micromolar, respectively”
Claim · model-written summary
Adenosine and other cytokinin nucleosides inhibited the hydrolysis of N6-(Delta2-isopentenyl)adenosine.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- adenosine and other cytokinin nucleosidese0
- Outcome
- hydrolysis of N(6)-(Delta(2)-isopentenyl)adenosinee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“The presence of adenosine and other cytokinin nucleosides inhibited the hydrolysis of N(6)-(Delta(2)-isopentenyl)adenosine”
Claim · model-written summary
The hydrolysis of N6-(Delta2-isopentenyl)adenosine was insensitive to guanosine, uridine and 3-deoxyadenosine.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- guanosine, uridine, or 3'-deoxyadenosinee1
- Outcome
- hydrolysis of N(6)-(Delta(2)-isopentenyl)adenosinee0
- Direction
- insensitivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat Germ Cells”
e0“The presence of adenosine and other cytokinin nucleosides inhibited the hydrolysis of N(6)-(Delta(2)-isopentenyl)adenosine”
e1“this reaction was insensitive to guanosine, uridine, or 3'-deoxyadenosine”
Claim · model-written summary
It is hypothesized that an adequate level of active cytokinin in plant cells may be provided through deribosylation of cytokinin riboside together with other cytokinin metabolic enzymes.
Verbatim
e0“It is hypothesized that an adequate level of "active cytokinin" in plant cells may be provided through the deribosylation of cytokinin riboside in concert with other cytokinin metabolic enzymes”
The same questions in other papers
adenosine · molecular: metabolites · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- negative
cordycepin · molecular: metabolites · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- no effect
class · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.