Paper · 1981 · Plant and Cell Physiology

Gibberellic Acid-stimulated de Novo Synthesis of a Particular Isozyme of Acid Phosphatase in Wheat Half-seeds

Takashi Akiyama; Hirofumi Uchimiya; Hiroshi Suzuki

5
claims
10
verbatim quotes
12
citations
2
research areas
—
Not open access

Abstract

GA3 stimulated incorporation of radioactive methionine into an acid phosphatase isozyme possessing an isoelectric pH at 4.0 (pI 4 isozyme). This effect of GA3 was completely inhibited by cycloheximide, but not by cordycepin. The results suggest the existence of preformed mRNA coding for pI 4 isozyme in the wheat aleurone layer and the stimulation of its translation by GA3.

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Claims 5

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

GA3 stimulated incorporation of radioactive methionine into the pI 4 acid phosphatase isozyme in wheat half-seeds.

Treatment
GA3e0
Gene
pI 4 isozymee0
Outcome
incorporation of radioactive methionine into an acid phosphatase isozymee0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Half-seeds”

✓ located verbatim in the source · title, characters 93–109 · show in text ↑
e0

“GA3 stimulated incorporation of radioactive methionine into an acid phosphatase isozyme possessing an isoelectric pH at 4.0 (pI 4 isozyme)”

✓ located verbatim in the source · abstract, characters 0–138 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cycloheximide completely inhibited the GA3 effect on methionine incorporation into the acid phosphatase isozyme.

Treatment
cycloheximidee1
Outcome
effect of GA3e1
Direction
inhibitede1
Comparator
GA3e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Half-seeds”

✓ located verbatim in the source · title, characters 93–109 · show in text ↑
e1

“This effect of GA3 was completely inhibited by cycloheximide, but not by cordycepin”

✓ located verbatim in the source · abstract, characters 140–223 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cordycepin did not inhibit the GA3 effect on the acid phosphatase isozyme.

Treatment
cordycepine1
Outcome
effect of GA3e1
Direction
no_changeinferrede1
Comparator
GA3e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Half-seeds”

✓ located verbatim in the source · title, characters 93–109 · show in text ↑
e1

“This effect of GA3 was completely inhibited by cycloheximide, but not by cordycepin”

✓ located verbatim in the source · abstract, characters 140–223 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The results suggest that preformed mRNA coding for the pI 4 isozyme exists in the wheat aleurone layer.

Organ / stage
aleurone layere2
Gene
pI 4 isozymee2
Outcome
preformed mRNA coding for pI 4 isozymee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Half-seeds”

✓ located verbatim in the source · title, characters 93–109 · show in text ↑
e2

“The results suggest the existence of preformed mRNA coding for pI 4 isozyme in the wheat aleurone layer and the stimulation of its translation by GA3”

✓ located verbatim in the source · abstract, characters 225–374 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The results suggest that GA3 stimulates translation of the preformed mRNA for the pI 4 isozyme.

Treatment
GA3e2
Gene
pI 4 isozymee2
Outcome
stimulation of its translatione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Half-seeds”

✓ located verbatim in the source · title, characters 93–109 · show in text ↑
e2

“The results suggest the existence of preformed mRNA coding for pI 4 isozyme in the wheat aleurone layer and the stimulation of its translation by GA3”

✓ located verbatim in the source · abstract, characters 225–374 · show in text ↑

cordycepin · molecular: metabolites · Triticum aestivum (wheat)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.