Abstract
A 2.4 kb fragment containing the 5'-flanking region and the 5'-noncoding sequence of the Vicia faba legumin gene LeB4 mediates high level seed-specific expression in transgenic tobacco plants. Deleted derivatives of this legumin upstream sequence were fused to the npt-II reporter gene to determine the tissue-specific activity of the chimeric constructs in stably transformed tobacco plants. The results indicate the presence of positive regulatory, enhancer-like cis elements within 566 bp of the upstream sequence. Most importantly, however, these elements are only fully functional in conjunction with the core motif CATGCATG of the legumin box around position -95, since destruction of the motif by a 6 bp deletion in an otherwise intact 2.4 kb upstream sequence drastically reduces expression in seeds. At the same time, low level expression in leaves is observed. The occurrence of similar CATGCATG consensus cis elements with alternating purine and pyrimidine base pairs in front of several other plant genes suggests a functional role of the motif in a wider range of plant promoters.
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Claims 5
Claim · model-written summary
The Vicia faba LeB4 promoter fragment mediated high seed-specific expression in tobacco.
- Taxon
- tobacco plants
Nicotiana tabacume0 - Material
- transgenic tobacco plantse0
- Organ / stage
- seede0
- Gene
- Vicia faba legumin gene LeB4e0
- Outcome
- seed-specific expressione0
- Direction
- high levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 2.4 kb fragment containing the 5'-flanking region and the 5'-noncoding sequence of the Vicia faba legumin gene LeB4 mediates high level seed-specific expression in transgenic tobacco plants”
Claim · model-written summary
Positive enhancer-like cis elements were identified within 566 bp upstream.
- Taxon
- tobacco plantsNicotiana tabacume1
- Gene
- positive regulatory, enhancer-like cis elementse0
- Outcome
- presence of positive regulatory, enhancer-like cis elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate the presence of positive regulatory, enhancer-like cis elements within 566 bp of the upstream sequence”
e1“stably transformed tobacco plants”
Claim · model-written summary
Deleting the CATGCATG motif drastically reduced seed expression.
- Taxon
- tobacco plantsNicotiana tabacume2
- Treatment
- 6 bp deletione0
- Gene
- core motif CATGCATGe1
- Outcome
- expression in seedse0
- Direction
- drastically reducese0
- Comparator
- otherwise intact 2.4 kb upstream sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“destruction of the motif by a 6 bp deletion in an otherwise intact 2.4 kb upstream sequence drastically reduces expression in seeds”
e1“core motif CATGCATG of the legumin box”
e2“stably transformed tobacco plants”
Claim · model-written summary
The motif deletion allowed low expression in leaves.
- Taxon
- tobacco plantsNicotiana tabacume2
- Organ / stage
- leavese0
- Gene
- core motif CATGCATGe1
- Outcome
- expression in leavese0
- Direction
- low levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the same time, low level expression in leaves is observed”
e1“core motif CATGCATG of the legumin box”
e2“stably transformed tobacco plants”
Claim · model-written summary
Related promoter motifs suggest a wider functional role.
- Taxon
- tobacco plantsNicotiana tabacume1
- Gene
- CATGCATG consensus cis elementse0
- Outcome
- a functional role of the motif in a wider range of plant promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence of similar CATGCATG consensus cis elements with alternating purine and pyrimidine base pairs in front of several other plant genes suggests a functional role of the motif in a wider range of plant promoters”
e1“stably transformed tobacco plants”
The same questions in other papers
loss of function · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
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- positive
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See all 50 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.