Abstract
Expression of the plastid rRNA operon (rrn) during development is highly regulated at the level of transcription. The plastid rrn operon in most higher plants is transcribed by the plastid-encoded RNA polymerase (PEP), the multisubunit plastid RNA polymerase from PrrnP1, a sigma(70)-type promoter with conserved -10 and -35 core promoter elements. To identify functionally important sequences, the tobacco PrrnP1 was dissected in vivo and in vitro. Based on in vivo deletion analysis, sequences upstream of nucleotide -83 do not significantly contribute to promoter function. The in vitro analyses identified an essential hexameric sequence upstream of the -35 element (GTGGGA; the rRNA operon upstream activator [RUA]) that is conserved in monocot and dicot species and suggested that the -10 element plays only a limited role in PrrnP1 recognition. Mutations in the initial transcribed sequence (+9 to +14) enhanced transcription, the characteristic of strong promoters in prokaryotes. We propose that sigma interaction with the -10 element in PrrnP1 is replaced in part by direct PEP-RUA (protein-DNA) interaction or by protein-protein interaction between the PEP and an RUA binding transcription factor.
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Claims 5
Claim · model-written summary
Sequences upstream of -83 did not significantly contribute to promoter function.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- promotere0
- Outcome
- promoter functione0
- Direction
- do not significantly contributee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on in vivo deletion analysis, sequences upstream of nucleotide -83 do not significantly contribute to promoter function.”
t0“Tobacco”
Claim · model-written summary
An essential upstream hexameric RUA sequence was identified and conserved across monocots and dicots.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- rRNA operon upstream activator [RUA]e0
- Outcome
- essential hexameric sequencee0
- Value
- GTGGGAe0
- Qualifier
- conserved in monocot and dicot speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro analyses identified an essential hexameric sequence upstream of the -35 element (GTGGGA; the rRNA operon upstream activator [RUA]) that is conserved in monocot and dicot species and suggested that the -10 element plays only a limited role in PrrnP1 recognition.”
t0“Tobacco”
Claim · model-written summary
The -10 element played only a limited role in PrrnP1 recognition.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- PrrnP1e0
- Outcome
- limited role in PrrnP1 recognitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro analyses identified an essential hexameric sequence upstream of the -35 element (GTGGGA; the rRNA operon upstream activator [RUA]) that is conserved in monocot and dicot species and suggested that the -10 element plays only a limited role in PrrnP1 recognition.”
t0“Tobacco”
Claim · model-written summary
Mutations at +9 to +14 enhanced transcription.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Mutations in the initial transcribed sequence (+9 to +14)e0
- Outcome
- transcriptione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in the initial transcribed sequence (+9 to +14) enhanced transcription, the characteristic of strong promoters in prokaryotes.”
t0“Tobacco”
Claim · model-written summary
The authors propose PEP-RUA or PEP-factor interactions partly replace sigma interaction with the -10 element.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- PrrnP1e0
- Outcome
- sigma interaction with the -10 element in PrrnP1e0
- Qualifier
- direct PEP-RUA (protein-DNA) interaction or by protein-protein interaction between the PEP and an RUA binding transcription factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that sigma interaction with the -10 element in PrrnP1 is replaced in part by direct PEP-RUA (protein-DNA) interaction or by protein-protein interaction between the PEP and an RUA binding transcription factor.”
t0“Tobacco”
The same questions in other papers
loss of function · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
13 papers in the same direction as this paper · 36 in the opposite direction
- negative
- negative
- negative
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See all 50 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.