Abstract
ABSTRACT We detected the generation of the reactive oxygen species (ROS) superoxide anion ( O.(-) (2)) and hydrogen peroxide (H(2)O(2)) in apple wounds 2 immediately after wounding, and assessed the relationships between (i) timely colonization of apple wounds by biocontrol yeasts, (ii) resistance of these microorganisms to oxidative stress caused by ROS, and (iii) their antagonism against postharvest wound pathogens. We analyzed a model system consisting of two yeasts with higher (Cryptococcus laurentii LS-28) or lower (Rhodotorula glutinis LS-11) antagonistic activity against the postharvest pathogens Botrytis cinerea and Penicillium expansum. LS-28 exhibited faster and greater colonization of wounds than LS-11. In contrast to LS-28, the number of LS-11 cells dropped 1 and 2 h after application, and then increased only later. In vitro, LS-28 was more resistant to ROS-generated oxidative stress. The combined application of biocontrol yeasts and ROS-deactivating enzymes in apple wounds prevented the decrease in number of LS-11 cells mentioned above, and enhanced colonization and antagonistic activity of both biocontrol yeasts against B. cinerea and P. expansum. Polar lipids of LS-11 contained the more unsaturated and oxidizable alpha-linolenic acid, which was absent in LS-28. Resistance to oxidative stress could be a key mechanism of biocontrol yeasts antagonism against postharvest wound pathogens.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Reactive oxygen species (superoxide anion and hydrogen peroxide) were generated in apple wounds immediately after wounding.
- Taxon
- apple
Malus domesticae0 - Organ / stage
- woundse0
- Outcome
- generation of the reactive oxygen species (ROS)e0
- Qualifier
- immediately after woundinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We detected the generation of the reactive oxygen species (ROS) superoxide anion ( O.(-) (2)) and hydrogen peroxide (H(2)O(2)) in apple wounds 2 immediately after wounding”
Claim · model-written summary
Cryptococcus laurentii LS-28 colonised apple wounds faster and more greatly than Rhodotorula glutinis LS-11.
- Taxon
- appleMalus domesticae1
- Interactor
- LS-28e2biocontrol_agent
- Outcome
- colonization of woundse2
- Direction
- faster and greatere2
- Comparator
- LS-11e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in apple wounds 2”
e2“LS-28 exhibited faster and greater colonization of wounds than LS-11”
Claim · model-written summary
The number of LS-11 cells dropped 1 and 2 h after application to apple wounds and then increased only later.
- Taxon
- appleMalus domesticae1
- Interactor
- LS-11e3biocontrol_agent
- Outcome
- number of LS-11 cellse3
- Direction
- droppede3
- Qualifier
- 1 and 2 h after applicatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in apple wounds 2”
e3“the number of LS-11 cells dropped 1 and 2 h after application, and then increased only later”
Claim · model-written summary
Combined application of biocontrol yeasts and ROS-deactivating enzymes prevented the decrease in LS-11 cell number in apple wounds.
- Taxon
- appleMalus domesticae4
- Treatment
- combined application of biocontrol yeasts and ROS-deactivating enzymese4
- Interactor
- LS-11e4biocontrol_agent
- Outcome
- number of LS-11 cellse4
- Direction
- prevented the decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The combined application of biocontrol yeasts and ROS-deactivating enzymes in apple wounds prevented the decrease in number of LS-11 cells”
Claim · model-written summary
Combined application of biocontrol yeasts and ROS-deactivating enzymes enhanced colonization and antagonistic activity of both yeasts against Botrytis cinerea and Penicillium expansum.
- Taxon
- appleMalus domesticae4
- Treatment
- combined application of biocontrol yeasts and ROS-deactivating enzymese4
- Interactor
- B. cinerea
Botrytis cinereae5pathogen - Second interactor
- P. expansum
Penicillium expansume5pathogen - Outcome
- colonization and antagonistic activitye5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The combined application of biocontrol yeasts and ROS-deactivating enzymes in apple wounds prevented the decrease in number of LS-11 cells”
e5“enhanced colonization and antagonistic activity of both biocontrol yeasts against B. cinerea and P. expansum”
Claim · model-written summary
Polar lipids of LS-11 contained more unsaturated alpha-linolenic acid than LS-28, in which it was absent.
- Taxon
- appleMalus domesticae1
- Interactor
- LS-11e6biocontrol_agent
- Outcome
- alpha-linolenic acide6
- Direction
- moree6
- Comparator
- LS-28e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in apple wounds 2”
e6“Polar lipids of LS-11 contained the more unsaturated and oxidizable alpha-linolenic acid, which was absent in LS-28”
Claim · model-written summary
Resistance to oxidative stress could be a key mechanism of biocontrol yeast antagonism against postharvest wound pathogens in apple.
- Taxon
- appleMalus domesticae1
- Outcome
- antagonism against postharvest wound pathogense7
- Qualifier
- Resistance to oxidative stresse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in apple wounds 2”
e7“Resistance to oxidative stress could be a key mechanism of biocontrol yeasts antagonism against postharvest wound pathogens”
Claim · model-written summary
In vitro, LS-28 was more resistant to ROS-generated oxidative stress.
- Taxon
- appleMalus domesticae1
- Interactor
- LS-28e2biocontrol_agent
- Outcome
- resistant to ROS-generated oxidative stresse2
- Direction
- moree2
- Setting
- In vitroe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in apple wounds 2”
e2“In vitro, LS-28 was more resistant to ROS-generated oxidative stress”
The same questions in other papers
biocontrol · Botrytis cinerea · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.