Abstract
Elicitation of defense reactions in tobacco by cryptogein, triggered a production of active oxygen species (AOS) via the NADPH oxidase, NtrbohD, and an accumulation of beta1din, a defense induced beta-type subunit of 20S proteasome. The proteasome inhibitor, MG132, stimulated this AOS production. Tobacco cells transformed with sense constructs of beta1din showed an inhibition of the AOS production following elicitin treatment, whereas the antisense transformed cells showed a strongly enhanced AOS production. In cells transformed with sense construct of beta1din, the NtrbohD transcripts failed to be induced by cryptogein as observed in control and antisense transformed cells. Conversely, in tobacco cells transformed with antisense constructs for NtrbohD, beta1din transcripts remained at a low level after elicitation. These results constitute the first demonstration of proteasome comprising beta1din acting as a negative regulator of NtrbohD and contributes to the regulation of AOS generation during plant defense reactions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Cryptogein elicited AOS production in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- cryptogeine0
- Gene
- NtrbohDe0
- Outcome
- production of active oxygen species (AOS)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elicitation of defense reactions in tobacco by cryptogein, triggered a production of active oxygen species (AOS) via the NADPH oxidase, NtrbohD, and an accumulation of beta1din, a defense induced beta-type subunit of 20S proteasome.”
Claim · model-written summary
Cryptogein induced beta1din accumulation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cryptogeine0
- Gene
- beta1dine0
- Outcome
- an accumulation of beta1dine0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elicitation of defense reactions in tobacco by cryptogein, triggered a production of active oxygen species (AOS) via the NADPH oxidase, NtrbohD, and an accumulation of beta1din, a defense induced beta-type subunit of 20S proteasome.”
Claim · model-written summary
MG132 stimulated AOS production.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- MG132e0
- Outcome
- AOS productione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proteasome inhibitor, MG132, stimulated this AOS production.”
e1“Elicitation of defense reactions in tobacco by cryptogein”
Claim · model-written summary
Sense beta1din transformation inhibited AOS production after elicitin treatment.
- Taxon
- TobaccoNicotiana tabacume0
- Treatment
- sense constructs of beta1dine0
- Gene
- beta1dine0
- Outcome
- AOS productione0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco cells transformed with sense constructs of beta1din showed an inhibition of the AOS production following elicitin treatment, whereas the antisense transformed cells showed a strongly enhanced AOS production.”
Claim · model-written summary
Antisense beta1din transformation strongly enhanced AOS production.
- Taxon
- TobaccoNicotiana tabacume0
- Treatment
- antisense transformed cellse0
- Gene
- beta1dine0
- Outcome
- AOS productione0
- Direction
- strongly enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco cells transformed with sense constructs of beta1din showed an inhibition of the AOS production following elicitin treatment, whereas the antisense transformed cells showed a strongly enhanced AOS production.”
Claim · model-written summary
NtrbohD transcripts were not induced by cryptogein in sense beta1din cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- sense construct of beta1dine0
- Gene
- NtrbohDe0
- Outcome
- NtrbohD transcriptse0
- Direction
- failed to be inducede0
- Comparator
- control and antisense transformed cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cells transformed with sense construct of beta1din, the NtrbohD transcripts failed to be induced by cryptogein as observed in control and antisense transformed cells.”
e1“tobacco cells transformed with antisense constructs for NtrbohD”
Claim · model-written summary
NtrbohD antisense cells retained low beta1din transcripts after elicitation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- antisense constructs for NtrbohDe0
- Gene
- beta1dine0
- Outcome
- beta1din transcriptse0
- Direction
- low levele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Conversely, in tobacco cells transformed with antisense constructs for NtrbohD, beta1din transcripts remained at a low level after elicitation.”
Claim · model-written summary
The beta1din proteasome acts as a negative regulator of NtrbohD and AOS generation.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- beta1dine0
- Outcome
- regulation of AOS generatione0
- Qualifier
- negative regulator of NtrbohDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results constitute the first demonstration of proteasome comprising beta1din acting as a negative regulator of NtrbohD and contributes to the regulation of AOS generation during plant defense reactions.”
e1“Elicitation of defense reactions in tobacco by cryptogein”
The same questions in other papers
loss of function · yield · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →transgenic · yield · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
8 papers more in this group
See all 12 papers →loss of function · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
35 papers in the same direction as this paper · 14 in the opposite direction
- positive
- positive
- positive
46 papers more in this group
See all 50 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.