Abstract
The accumulation of cinnamoyl putrescines by cell suspension cultures of NICOTIANA TABACUM was enhanced manifold by phosphate limitation of the culture medium while growth was reduced under such conditions. The enhanced product formation was preceded by a large increase and subsequent decline of phenylalanine ammonia lyase activity and a smaller increase of the activity of ornithine decarboxylase. Phosphate concentrations commonly used in cell culture media suppressed the product accumulation.
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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Phosphate limitation greatly enhanced cinnamoyl putrescine accumulation in tobacco cell cultures.
- Taxon
- NICOTIANA TABACUM
Nicotiana tabacume0 - Treatment
- phosphate limitatione0
- Outcome
- accumulation of cinnamoyl putrescinese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of cinnamoyl putrescines by cell suspension cultures of NICOTIANA TABACUM was enhanced manifold by phosphate limitation of the culture medium”
Claim · model-written summary
Phosphate limitation reduced tobacco cell culture growth.
- Taxon
- NICOTIANA TABACUMNicotiana tabacumt0
- Treatment
- phosphate limitatione1
- Outcome
- growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while growth was reduced under such conditions.”
e1“The accumulation of cinnamoyl putrescines by cell suspension cultures of NICOTIANA TABACUM was enhanced manifold by phosphate limitation of the culture medium while growth was reduced under such conditions.”
t0“Nicotiana tabacum”
Claim · model-written summary
Enhanced product formation was preceded by a large rise and later decline in phenylalanine ammonia lyase activity.
- Taxon
- NICOTIANA TABACUMNicotiana tabacumt0
- Gene
- phenylalanine ammonia lyasee0
- Outcome
- phenylalanine ammonia lyase activitye0
- Direction
- increase and subsequent declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enhanced product formation was preceded by a large increase and subsequent decline of phenylalanine ammonia lyase activity”
t0“Nicotiana tabacum”
Claim · model-written summary
Enhanced product formation was preceded by a smaller increase in ornithine decarboxylase activity.
- Taxon
- NICOTIANA TABACUMNicotiana tabacumt0
- Gene
- ornithine decarboxylasee0
- Outcome
- activity of ornithine decarboxylasee0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a smaller increase of the activity of ornithine decarboxylase.”
t0“Nicotiana tabacum”
Claim · model-written summary
Phosphate concentrations commonly used in cell culture media suppressed product accumulation.
- Taxon
- NICOTIANA TABACUMNicotiana tabacumt0
- Treatment
- Phosphate concentrations commonly used in cell culture mediae0
- Outcome
- product accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phosphate concentrations commonly used in cell culture media suppressed the product accumulation.”
t0“Nicotiana tabacum”
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