Abstract
The effects of calcium ions on lysine transport into cultured Wisconsin-38 tobacco cells were examined. In the presence of calcium, lysine was transported at a relatively low rate for 30 to 40 minutes followed by a period of increasing rates and subsequent stabilization at a higher rate after 2 to 3 hours. In the absence of calcium, transport was uniformly low.Time-dependent stimulation of lysine transport rate was observed after the cells had been preincubated in calcium-containing media. Similar treatments also resulted in the stimulated uptake of a variety of other amino acids, organic compounds, and sulfate. The stimulation of lysine uptake was apparently not due to nutrient starvation.Lysine transport was not stimulated in a time-dependent fashion by K(+), La(3+), Mg(2+), or Mn(2+). Cells with stimulated transport rates continued to exhibit high rates when washed with calcium-containing media followed by transport in calcium-containing media. All other cation wash treatments were inhibitory, although magnesium treatments resulted in partial preservation of stimulated transport rates. Cycloheximide inhibited the calcium/time-dependent stimulation of lysine transport and caused the stimulated rate to decay.The initial experimental treatments or the culture conditions may represent some form of shock that alters the membrane transport mechanism, thus reducing transport. The observed calcium/time-dependent stimulation may require protein synthesis and represents damage repair.
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Claims 9
Claim · model-written summary
With calcium, lysine transport rose after an initial low-rate interval and stabilized by 2 to 3 hours.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- calciume0
- Outcome
- lysine was transportede0
- Direction
- increasinge0
- Qualifier
- after 2 to 3 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“In the presence of calcium, lysine was transported at a relatively low rate for 30 to 40 minutes followed by a period of increasing rates and subsequent stabilization at a higher rate after 2 to 3 hours.”
Claim · model-written summary
Without calcium lysine transport stayed uniformly low.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- absence of calciume0
- Outcome
- transporte0
- Direction
- uniformly lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“In the absence of calcium, transport was uniformly low.”
Claim · model-written summary
Calcium preincubation stimulated lysine transport rate over time.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- preincubated in calcium-containing mediae0
- Outcome
- lysine transport ratee0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Time-dependent stimulation of lysine transport rate was observed after the cells had been preincubated in calcium-containing media.”
Claim · model-written summary
Similar treatment stimulated uptake of other amino acids, organic compounds and sulfate.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Similar treatmentse0
- Outcome
- uptake of a variety of other amino acids, organic compounds, and sulfatee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Similar treatments also resulted in the stimulated uptake of a variety of other amino acids, organic compounds, and sulfate.”
Claim · model-written summary
Nutrient starvation apparently did not account for stimulated lysine uptake.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- stimulation of lysine uptakee0
- Qualifier
- not due to nutrient starvatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“The stimulation of lysine uptake was apparently not due to nutrient starvation.”
Claim · model-written summary
K, La, Mg and Mn did not produce time-dependent lysine transport stimulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- K(+), La(3+), Mg(2+), or Mn(2+)e0
- Outcome
- Lysine transporte0
- Direction
- not stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Lysine transport was not stimulated in a time-dependent fashion by K(+), La(3+), Mg(2+), or Mn(2+).”
Claim · model-written summary
Calcium wash preserved high stimulated transport rates.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- washed with calcium-containing mediae0
- Outcome
- transport ratese0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Cells with stimulated transport rates continued to exhibit high rates when washed with calcium-containing media followed by transport in calcium-containing media.”
Claim · model-written summary
Other cation washes inhibited transport, while magnesium partly preserved stimulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- other cation wash treatmentse0
- Outcome
- transport ratese0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“All other cation wash treatments were inhibitory, although magnesium treatments resulted in partial preservation of stimulated transport rates.”
Claim · model-written summary
Cycloheximide inhibited calcium-dependent lysine transport stimulation and caused its decay.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Cycloheximidee0
- Outcome
- calcium/time-dependent stimulation of lysine transporte0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Cycloheximide inhibited the calcium/time-dependent stimulation of lysine transport and caused the stimulated rate to decay.”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.